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公开(公告)号：US20110159499A1
公开(公告)日：2011-06-30
申请号：US12954634
申请日：2010-11-25
申请人： Benajamin HINDSON , Serge Saxonov , Phillip Belgrader , Kevin Ness , Michael Lucero , Billy Colston
发明人： Benajamin HINDSON , Serge Saxonov , Phillip Belgrader , Kevin Ness , Michael Lucero , Billy Colston
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q2537/157 , C12Q2563/161 , C12Q2533/107 , C12Q2521/319
摘要： This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
摘要翻译： 本发明提供了用于检测靶多核苷酸拷贝数差异的组合物和方法。 在一些情况下，当起始样品是母体组织（例如血液，血浆）时，本文提供的方法和组合物可用于诊断胎儿遗传异常。 描述的方法和材料适用于允许检测多核苷酸拷贝数的小但统计学上显着的差异的技术。
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公开(公告)号：US20120252015A1
公开(公告)日：2012-10-04
申请号：US13400030
申请日：2012-02-17
申请人： Benjamin Hindson , Serge Saxonov , Philip Belgrader , Kevin Ness , Michael Lucero , Billy Colston , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
发明人： Benjamin Hindson , Serge Saxonov , Philip Belgrader , Kevin Ness , Michael Lucero , Billy Colston , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
CPC分类号： C12Q1/6883 , C12Q2521/331 , C12Q2523/125 , C12Q2600/154
摘要： The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma).
摘要翻译： 本公开提供了用于检测样品中的多核苷酸并用于定量样品中的多核苷酸负载的方法和组合物。 多核苷酸可以与疾病，病症或病症相关。 在一些应用中，例如为了确定样品中多核苷酸的负载量化甲基化DNA。 本公开还提供了用于确定生物样品中胎儿多核苷酸负荷的方法和组合物，例如母体血浆中胎儿多核苷酸（例如DNA，RNA）的负载。 本公开提供了用于检测细胞过程如细胞活力，生长速率和感染率的方法和组合物。 本公开还提供了用于检测靶多核苷酸的拷贝数差异的组合物和方法。 在一些实施方案中，当起始样品是母体组织（例如血液，血浆）时，本文提供的方法和组合物可用于诊断胎儿遗传异常。
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公开(公告)号：US20110189678A1
公开(公告)日：2011-08-04
申请号：US12859176
申请日：2010-08-18
CPC分类号： B01L3/00 , B01F13/0059 , B01J2219/00337 , B01J2219/00353 , B01J2219/00403 , B01J2219/00495 , B01J2219/00585 , B01J2219/0059 , B01J2219/0072 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/50851 , B01L2300/0627 , B01L2300/0883 , B01L2300/123 , B01L2400/06 , C12Q1/6844 , C40B60/08 , C40B60/14 , G01N35/00029 , G01N35/0098 , G01N2035/00366 , Y10T436/25 , C12Q2565/501
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
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公开(公告)号：US07749737B2
公开(公告)日：2010-07-06
申请号：US11929436
申请日：2007-10-30
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M次.N基质微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US07666361B2
公开(公告)日：2010-02-23
申请号：US10818642
申请日：2004-04-05
申请人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Ramez Nassef , Geoff Facer , Yong Yi
发明人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Ramez Nassef , Geoff Facer , Yong Yi
IPC分类号： B01L3/00
CPC分类号： B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US20050129581A1
公开(公告)日：2005-06-16
申请号：US10818642
申请日：2004-04-05
申请人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
发明人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
CPC分类号： B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US09103825B2
公开(公告)日：2015-08-11
申请号：US12764819
申请日：2010-04-21
申请人： Hany Ramez Nassef , Hou-Pu Chou , Michael Lucero , Andrew May , Kathy Yokobata
发明人： Hany Ramez Nassef , Hou-Pu Chou , Michael Lucero , Andrew May , Kathy Yokobata
IPC分类号： B01L3/00 , G01N33/543 , F16K99/00 , G01N33/53
CPC分类号： G01N33/54366 , B01J2219/00286 , B01J2219/00317 , B01J2219/00389 , B01J2219/00398 , B01L3/5025 , B01L3/502715 , B01L3/502738 , B01L2300/0816 , B01L2300/0874 , B01L2300/123 , B01L2400/0487 , B01L2400/0655 , F16K99/0001 , F16K99/0015 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N33/5302
摘要： A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.
摘要翻译： 微流体装置适于执行许多同时结合测定，包括但不限于免疫学实验，例如ELISA测定，初级和第二抗体之间的最小串扰。
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公开(公告)号：US20130171633A1
公开(公告)日：2013-07-04
申请号：US13548068
申请日：2012-07-12
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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9.发明申请STRUCTURAL RING INTERCONNECT PRINTED CIRCUIT BOARD ASSEMBLY FOR A DUCTED FAN UNMANNED AERIAL VEHICLE 失效结构环形互连电路板组件，用于风扇无人驾驶的空中客车公开(公告)号：US20100120273A1
公开(公告)日：2010-05-13
申请号：US12270196
申请日：2008-11-13
IPC分类号： H01R12/00
CPC分类号： H05K1/02 , B64C2201/027 , H05K1/14 , H05K2201/09027 , H05K2201/09063
摘要： A structural ring interconnect printed circuit board assembly for a ducted fan unmanned aerial vehicle that comprises a printed circuit board attached to a core vehicle body, wherein the printed circuit board conforms to the shape of the core vehicle body's airframe skirt, and wherein the printed circuit board provides structural integrity to the core vehicle body.
摘要翻译： 一种用于管道风扇无人机的结构环互连印刷电路板组件，其包括附接到核心车体的印刷电路板，其中印刷电路板符合核心车身的机身裙部的形状，并且其中印刷电路 主板为核心车身提供了结构完整性。
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公开(公告)号：US20080230387A1
公开(公告)日：2008-09-25
申请号：US11929625
申请日：2007-10-30
IPC分类号： G01N27/26
CPC分类号： B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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