公开(公告)号：US20060029955A1

公开(公告)日：2006-02-09

申请号：US11175789

申请日：2005-07-05

申请人： Antonio Guia ,  George Walker ,  Mingxian Huang ,  Abdelleh Menikh ,  Lei Wu ,  Sithiphong Khachonesin ,  Julian Yuan ,  Jia Xu

发明人： Antonio Guia ,  George Walker ,  Mingxian Huang ,  Abdelleh Menikh ,  Lei Wu ,  Sithiphong Khachonesin ,  Julian Yuan ,  Jia Xu

IPC分类号： C12Q1/68 ,  G01N33/567

CPC分类号： G01N33/48728 ,  B01L3/502761 ,  B01L3/502792 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/161 ,  B01L2400/049 ,  B03C5/026 ,  B65B31/00 ,  C12Q2565/629 ,  C23C16/452 ,  F16J15/021 ,  G01N15/1031 ,  G01N31/22 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2333/705 ,  H01J2237/3142

摘要： The present invention includes biochips for the measurement of cellular ion channels and methods of use and manufacture. The biochips of the present invention have enhanced sealing capabilities provided in part by chemically modifying the surface of the biochip surface or substrate or by exposure to an ionized gas. The present invention also includes novel cartridges for biochips.

公开(公告)号：US20060024732A1

公开(公告)日：2006-02-02

申请号：US11230411

申请日：2005-09-20

申请人： Mingxian Huang ,  Lei Wu ,  Xiaobo Wang ,  Junquan Xu ,  Guo Liang Tao ,  David Rothwarf

发明人： Mingxian Huang ,  Lei Wu ,  Xiaobo Wang ,  Junquan Xu ,  Guo Liang Tao ,  David Rothwarf

IPC分类号： C12Q1/68

CPC分类号： C40B40/12 ,  B01J19/0046 ,  B01J2219/00457 ,  B01J2219/00468 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00556 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00734 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2400/0415 ,  B01L2400/043 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54366 ,  G01N33/54386 ,  G06K19/06009 ,  G06K19/06046 ,  G06K19/06196 ,  Y10T428/24917

摘要： This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

公开(公告)号：US06989086B2

公开(公告)日：2006-01-24

申请号：US09905755

申请日：2001-07-13

申请人： Jing Cheng ,  Edward L. Sheldon, III ,  Lei Wu ,  James P. O'Connell

发明人： Jing Cheng ,  Edward L. Sheldon, III ,  Lei Wu ,  James P. O'Connell

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： B01L3/5027 ,  B01D57/02 ,  B01J19/0093 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  C12N13/00 ,  G01N27/44743 ,  H01L29/6656

摘要： The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) % includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

摘要翻译： 本发明包括用于通过介电电泳，DC分离细胞的DC高压脉冲电离裂解，细胞裂解物等粗制混合物分离所需组分和/或这些裂解物的酶反应来进行细胞颗粒的无通道分离的装置和方法 所有这些都可以在单个生物电子芯片上进行。 本发明的优选实施例包括在印刷电路板（14）上包括微细硅芯片（12）的盒（10）和安装到芯片（12）上以形成流动室的流动池（16）。 盒（10）还包括用于将盒（10）电连接到电子控制器的输出销（22）。 芯片（12）％包括多个圆形微电极（24），其优选地涂覆有保护性渗透层。 分离来自各种细胞混合物的特异性细胞，裂解，并在芯片上酶消化。

公开(公告)号：US06403367B1

公开(公告)日：2002-06-11

申请号：US09470448

申请日：1999-12-22

申请人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

发明人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： C12M134

CPC分类号： G11C13/0019 ,  B01D57/02 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  C07H21/00 ,  C07K1/045 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6832 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N27/44743 ,  G11C13/0014 ,  G11C13/04 ,  G11C19/00 ,  H01L25/50 ,  H01L2924/0002 ,  C12Q2565/515 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2525/107 ,  C12Q2531/119 ,  C12Q2531/101 ,  C12Q2531/113 ,  H01L2924/00

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。