-
公开(公告)号：US20190242795A1
公开(公告)日：2019-08-08
申请号：US16236116
申请日：2018-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Billy W. Colston, JR. , Kevin D. Ness , Donald A. Masquelier
IPC: G01N1/30 , B01J19/00 , C12Q1/6876 , G01N1/44
Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
-
公开(公告)号：US10258988B2
公开(公告)日：2019-04-16
申请号：US15365883
申请日：2016-11-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Benjamin J. Hindson , Billy W. Colston, Jr. , Donald A. Masquelier
IPC: B01L3/00 , B01F3/08 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , C12Q1/686 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49 , B29L31/00
Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.
-
公开(公告)号：US10167509B2
公开(公告)日：2019-01-01
申请号：US14848311
申请日：2015-09-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/68 , C07H21/00 , C12Q1/6883 , C12Q1/683 , C12Q1/6858
Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.
-
公开(公告)号：US10166522B2
公开(公告)日：2019-01-01
申请号：US14938470
申请日：2015-11-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Billy W. Colston, Jr. , Kevin D. Ness , Donald A. Masquelier
IPC: C12Q1/68 , B01J19/00 , G01N21/75 , C12Q1/6844 , G01N33/50 , B01F13/00 , B01L3/00 , G01N35/08 , B01L7/00 , G01N1/30 , G01N1/44 , C07H21/02 , C40B50/08 , C40B60/14
Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
-
公开(公告)号：US20180135100A1
公开(公告)日：2018-05-17
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/6804 , C12Q1/6844 , C12Q1/6851
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
-
Patent Agency Ranking
公开(公告)号：US09764322B2
公开(公告)日：2017-09-19
申请号：US15351354
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Kevin D. Ness , Benjamin J. Hindson , Donald A. Masquelier , Erin R. Chia
IPC: B01L3/00 , B01F3/00 , B01F3/08 , B01L9/00 , C12Q1/68 , G01N35/08 , B01F13/00 , B01F5/00 , B01F5/02
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
-
公开(公告)号：US20170144160A1
公开(公告)日：2017-05-25
申请号：US15365883
申请日：2016-11-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Benjamin J. Hindson , Billy W. Colston, JR. , Donald A. Masquelier
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.
-
公开(公告)号：US20170056886A1
公开(公告)日：2017-03-02
申请号：US15351335
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well. The device also may include a channel network having a first channel, a second channel, and a third channel that meet one another in a droplet-generation region. The system also may comprise a holder for the device. The system further may comprise an instrument configured to operatively receive an assembly including the device and the holder and to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel.
Abstract translation: 用于形成乳液的系统，包括方法，装置和试剂盒。 示例性系统可以包括包括样品阱的样品，该样品井被配置成接收含有样品的流体，连续相良好地配置成接收连续相流体和液滴阱。 该设备还可以包括具有在液滴生成区域中彼此相遇的第一通道，第二通道和第三通道的通道网络。 该系统还可以包括用于该装置的保持器。 系统还可以包括被配置为可操作地接收包括装置和保持器的组件并且经由第一通道将样品容纳流体从样品阱驱动到液滴产生区域的仪器，来自连续相的连续相流体 通过第二通道对液滴产生区域以及通过第三通道从液滴产生区域到液滴阱的样品含有液滴。
-
公开(公告)号：US20160108464A1
公开(公告)日：2016-04-21
申请号：US14981134
申请日：2015-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Serge Saxonov , Simant Dube , Benjamin J. Hindson , Adam M. McCoy
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6851 , G06F17/10 , G06F17/11 , G06F17/12 , G16B5/00 , G16B20/00 , G16B25/00 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159
Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.
Abstract translation: 用于通过组合使用信号对更多数量目标进行多路复用数字测定的系统，包括方法，装置和组合物。
-
公开(公告)号：US20130302792A1
公开(公告)日：2013-11-14
申请号：US13945661
申请日：2013-07-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Billy W. Colston, JR. , Kevin D. Ness , Donald A. Masquelier
IPC: G01N21/64
CPC classification number: G01N21/6486 , C12Q1/6848 , G01N21/6428 , G01N21/6456 , G01N2021/6441 , G01N2201/1242 , C12Q2545/101 , C12Q2563/107 , C12Q2563/159
Abstract: System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.
Abstract translation: 用于执行使用从液滴检测的信号来控制和/或校准的基于液滴的测定的系统，包括方法和装置。
-
-
-
-
-
-
-
-
-
Search Results
50
records
(took 0.034 seconds)
