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11.发明申请Protein Crystallization Screening and Optimization Droplet Actuators, Systems and Methods 有权蛋白质结晶筛选和优化滴液致动器，系统和方法公开(公告)号：US20080044914A1
公开(公告)日：2008-02-21
申请号：US11839744
申请日：2007-08-16
申请人： Vamsee Pamula , Vijay Srinivasan , Michael Pollack
发明人： Vamsee Pamula , Vijay Srinivasan , Michael Pollack
IPC分类号： G01N33/68
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.
摘要翻译： 提供蛋白质结晶筛选和优化液滴致动器，系统和方法。 根据一个实施例，提供了一种筛选液滴致动器，其包括：（a）用于引入一种或多种结晶试剂和/或一种或多种蛋白质溶液的端口; 和（b）基材，包括：（i）两个或更多个混合孔的阵列; 和（ii）用于将包含结晶试剂和蛋白质溶液的液滴移动到混合孔中的电场介导的微流体。 还提供了优化液滴致动器，包括筛选液滴致动器的系统，筛选蛋白质结晶条件的方法以及测试晶体生长条件的方法。
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公开(公告)号：US20070275415A1
公开(公告)日：2007-11-29
申请号：US11639663
申请日：2006-12-15
IPC分类号： G01N33/573 , G01N33/48
CPC分类号： G01N33/54373 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502761 , B01L3/502784 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/148 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0421 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.
摘要翻译： 本发明涉及基于液滴的亲和力测定。 根据一个实施方案，提供了一种检测样品中的目标分析物的方法，其中所述方法包括：（a）执行液滴操作，以将基于亲和性的测定试剂与液滴微致动器与潜在地包含目标分析物的样品组合以产生 指示分析物的存在，不存在和/或数量的信号; 和（b）检测信号，其中信号对应于样品中分析物的存在，不存在和/或数量。
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公开(公告)号：US08883513B2
公开(公告)日：2014-11-11
申请号：US13030418
申请日：2011-02-18
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译： 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。
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公开(公告)号：US20080006535A1
公开(公告)日：2008-01-10
申请号：US11838388
申请日：2007-08-14
申请人： Philip Paik , Michael Pollack , Ryan Sturmer , Gregory Smith , Keith Brafford , Vamsee Pamula
发明人： Philip Paik , Michael Pollack , Ryan Sturmer , Gregory Smith , Keith Brafford , Vamsee Pamula
IPC分类号： G01N27/00
CPC分类号： B01L3/502792 , B01L2200/143 , B01L2300/027 , B01L2300/0816 , B01L2400/0427
摘要： Systems for controlling a droplet microactuator are provided. According to one embodiment, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, and a display device displaying a user interface electronically coupled to the controller, wherein the system is programmed and configured to permit a user to effect a droplet manipulation by interacting with the user interface. According to another embodiment, a system is provided and includes a processor, a display device electronically coupled to the processor, and software loaded and/or stored in a storage device electronically coupled to the controller, a memory device electronically coupled to the controller, and/or the controller and programmed to display an interactive map of a droplet microactuator. According to yet another embodiment, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, a display device displaying a user interface electronically coupled to the controller, and software for executing a protocol loaded and/or stored in a storage device electronically coupled to the controller, a memory device electronically coupled to the controller, and/or the controller.
摘要翻译： 提供了用于控制液滴微致动器的系统。 根据一个实施例，提供了一种系统，其包括电子耦合到控制器的控制器，液滴微致动器以及显示电子耦合到控制器的用户界面的显示设备，其中该系统被编程和配置为允许用户实现 通过与用户界面交互来进行液滴操作。 根据另一个实施例，提供了一种系统，包括处理器，电子耦合到处理器的显示设备，以及加载和/或存储在电子耦合到控制器的存储设备中的软件，电子耦合到控制器的存储设备，以及 /或控制器，并被编程以显示液滴微致动器的交互式地图。 根据另一个实施例，提供了一种系统，其包括控制器，电子耦合到控制器的液滴微致动器，显示电子耦合到控制器的用户界面的显示设备，以及用于执行加载和/或存储在控制器中的协议的软件 电子耦合到控制器的存储设备，电子耦合到控制器的存储设备和/或控制器。
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公开(公告)号：US20070217956A1
公开(公告)日：2007-09-20
申请号：US11753318
申请日：2007-05-24
申请人： Vamsee Pamula , Michael Pollack , Richard Fair
发明人： Vamsee Pamula , Michael Pollack , Richard Fair
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Methods for amplifying nucleic acid, such as DNA or RNA, on a printed circuit board (PCB) substrate are disclosed. The amplification can be achieved through a variety of methods such as thermocycling or isothermally. The printed circuit board substrate can comprise an array of electrodes for transporting droplets and can be part of a sandwich structure including a top plate parallel to the printed circuit board substrate.
摘要翻译： 公开了在印刷电路板（PCB）衬底上放大核酸如DNA或RNA的方法。 扩增可以通过各种方法如热循环或等温进行。 印刷电路板基板可以包括用于输送液滴的电极阵列，并且可以是包括平行于印刷电路板基板的顶板的夹层结构的一部分。
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公开(公告)号：US20080038810A1
公开(公告)日：2008-02-14
申请号：US11639990
申请日：2006-12-15
申请人： Michael Pollack , Philip Paik , Vamsee Pamula
发明人： Michael Pollack , Philip Paik , Vamsee Pamula
CPC分类号： B01L3/502761 , B01L3/5025 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/147 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2035/1046 , Y10T29/49002 , Y10T137/0318
摘要： The present invention relates to a droplet-based nucleic acid amplification device, system, and method. According to one embodiment, a droplet microactuator is provided and includes: (a) a substrate comprising electrodes for conducting droplet operations; and (b) one or more temperature control means arranged in proximity with one or more of the electrodes for heating and/or cooling a region of the droplet microactuator and arranged such that a droplet can be transported on the electrodes into the region for heating.
摘要翻译： 本发明涉及一种基于液滴的核酸扩增装置，系统和方法。 根据一个实施例，提供液滴微致动器，其包括：（a）包括用于进行液滴操作的电极的基板; 和（b）设置在一个或多个电极附近的一个或多个温度控制装置，用于加热和/或冷却液滴微致动器的区域，并且布置成使得液滴可以在电极上传送到加热区域中。
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公开(公告)号：US20070037294A1
公开(公告)日：2007-02-15
申请号：US11585491
申请日：2006-10-23
申请人： Vamsee Pamula , Michael Pollack , Richard Fair
发明人： Vamsee Pamula , Michael Pollack , Richard Fair
IPC分类号： G01N1/10
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.
摘要翻译： 提供了执行微流体取样的方法。 该方法包括提供包括第一，第二和第三电极的布置的基板，其中第二电极介于第一和第三电极之间。 该方法还包括使流体输入连续地流动到第一电极并且将第一，第二和第三电极偏压到第一电压以使流体输入的一部分扩展穿过第二和第三电极。 该方法还包括将第二电极偏压到不同于第一电压的第二电压，以在第三电极上形成液滴，液滴与流体输入分离。
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公开(公告)号：US20060054503A1
公开(公告)日：2006-03-16
申请号：US11077569
申请日：2005-03-10
申请人： Vamsee Pamula , Michael Pollack , Philip Paik , Hong Ren , Richard Fair
发明人： Vamsee Pamula , Michael Pollack , Philip Paik , Hong Ren , Richard Fair
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
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公开(公告)号：US20070045117A1
公开(公告)日：2007-03-01
申请号：US11585618
申请日：2006-10-23
申请人： Vamsee Pamula , Michael Pollack , Hong Ren , Richard Fair
发明人： Vamsee Pamula , Michael Pollack , Hong Ren , Richard Fair
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Apparatuses for mixing droplets, such as a binary mixing apparatus, are provided. The binary mixing apparatus includes an array of electrodes and a conducting element positioned in relation to at least one of the electrodes to enable a droplet placed in electrical communication with the at least one electrode to electrically communicate with the conducting element. The binary mixing apparatus additionally includes an electrode selector for sequentially biasing one or more selected electrodes of the array to move a droplet disposed on the array into contact with another droplet. The apparatus further includes a first droplet supply area communicating with the array and a second droplet supply area communicating with the array.
摘要翻译： 提供了用于混合液滴的装置，例如二元混合装置。 二进制混合装置包括电极阵列和相对于至少一个电极定位的导电元件，使得与至少一个电极电连通的液滴能够与导电元件电连通。 二进制混合装置还包括用于顺序地偏置阵列的一个或多个所选择的电极的电极选择器，以移动设置在阵列上的液滴与另一液滴接触。 该装置还包括与阵列连通的第一液滴供应区域和与阵列连通的第二液滴供应区域。
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公开(公告)号：US20060194331A1
公开(公告)日：2006-08-31
申请号：US11343284
申请日：2006-01-30
申请人： Vamsee Pamula , Michael Pollack , Richard Fair
发明人： Vamsee Pamula , Michael Pollack , Richard Fair
IPC分类号： G05D19/00
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.
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