中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

31 records (took 0.038 seconds)

    Multiphase nucleic acid amplification
    12.
    发明授权
    Multiphase nucleic acid amplification 有权
    多相核酸扩增

    公开(公告)号：US09139870B2

    公开(公告)日：2015-09-22

    申请号：US14014607

    申请日：2013-08-30

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

    IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/70

    CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

    Abstract translation: 公开了用于核酸扩增的改进方法，包括多重扩增，其中扩增在两个或多个不同阶段中进行。 第一相扩增反应优选缺少指数扩增所需的一种或多种组分。 随后在第二，第三或另外的扩增相中提供缺乏的组分，导致快速指数扩增反应。 多相协议在低分析物浓度下导致更快更灵敏的检测和更低的变异性。 还公开了用于实施所要求保护的方法的组合物。

    Methods for amplifying nucleic acid using tag-mediated displacement
    13.
    发明授权
    Methods for amplifying nucleic acid using tag-mediated displacement 有权

    公开(公告)号：US11840716B2

    公开(公告)日：2023-12-12

    申请号：US17135878

    申请日：2020-12-28

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

    IPC: C12P19/34 ,  C12Q1/6855 ,  C12Q1/6844

    CPC classification number: C12P19/34 ,  C12Q1/6855 ,  C12Q1/6844 ,  C12Q2537/137

    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

    Methods and compositions for nucleic acid amplification
    14.
    发明授权
    Methods and compositions for nucleic acid amplification 有权

    公开(公告)号：US10415092B2

    公开(公告)日：2019-09-17

    申请号：US15417736

    申请日：2017-01-27

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

    IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

    METHODS FOR AMPLIFYING NUCLEIC ACID USING TAG-MEDIATED DISPLACEMENT
    19.
    发明申请
    METHODS FOR AMPLIFYING NUCLEIC ACID USING TAG-MEDIATED DISPLACEMENT 有权

    公开(公告)号：US20210214759A1

    公开(公告)日：2021-07-15

    申请号：US17135878

    申请日：2020-12-28

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

    IPC: C12P19/34 ,  C12Q1/6855

    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

Patent Agency Ranking