公开(公告)号：US11878299B2

公开(公告)日：2024-01-23

申请号：US17169181

申请日：2021-02-05

Applicant: Illumina, Inc.

Inventor： Hui Han ,  Dajun Yuan ,  M. Shane Bowen

IPC: B01L3/00 ,  B29C65/16 ,  B29C65/14 ,  H01J37/32 ,  G03F7/038 ,  G03F7/00 ,  B81C1/00 ,  B32B17/10 ,  B32B17/06 ,  G03F7/30 ,  G03F7/16 ,  G03F7/20

CPC classification number: B01L3/502707 ,  B29C65/1406 ,  B29C65/1612 ,  B32B17/06 ,  B32B17/10 ,  B81C1/0038 ,  G03F7/0002 ,  G03F7/038 ,  H01J37/32009 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/0896 ,  B81C2201/0153 ,  G03F7/16 ,  G03F7/2002 ,  G03F7/30 ,  H01J2237/3341

Abstract: Imprinted substrates are often used to produce miniaturized devices for use in electrical, optic and biochemical applications. Imprinting techniques, such as nanoimprinting lithography, may leave residues in the surface of substrates that affect bonding and decrease the quality of the produced devices. An imprinted substrate with residue-free region, or regions with a reduced amount of residue for improved bonding quality is introduced. Methods to produce imprinted substrates without residues from the imprinting process are also introduced. Methods include physical exclusion methods, selective etching methods and energy application methods. These methods may produce residue-free regions in the surface of the substrate that can be used to produce higher strength bonding.

公开(公告)号：US20230279469A1

公开(公告)日：2023-09-07

申请号：US18146320

申请日：2022-12-23

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC. ,  Illumina Singapore Pte. Ltd.

Inventor： Johan Sebastian Basuki ,  Jonathan Mark Boutell ,  Jeffrey S. Fisher ,  Louise Jane Fraser ,  Wayne N. George ,  Niall Anthony Gormley ,  David Jones ,  Xiaoyu Ma ,  Maria Ines Martins Vitoriano ,  Zhong Mei ,  Oliver Jon Miller ,  Andrew Price ,  Sebastien Georg Gabriel Ricoult ,  Vicki S. Thomson ,  Jacqueline C. Weir ,  Xiaoy Ma ,  Weihua Chang ,  Hui Han

IPC: C12Q1/6806 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01L3/502761 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2300/0893

Abstract: An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.

公开(公告)号：US11249025B2

公开(公告)日：2022-02-15

申请号：US16482630

申请日：2018-01-31

Applicant: Illumina, Inc.

Inventor： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC: G01N21/64

Abstract: Fiducial markers are provided on a patterned array of the type that may be used for molecular analysis, such as sequencing. The fiducial markers may have configurations and layouts that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducial markers may include non-rectilinear layouts that may provide for more robust location of both the fiducial markers and sites of the patterned array.

公开(公告)号：US20210170400A1

公开(公告)日：2021-06-10

申请号：US17169181

申请日：2021-02-05

Applicant: Illumina, Inc.

Inventor： Hui Han ,  Dajun Yuan ,  M. Shane Bowen

IPC: B01L3/00 ,  B29C65/16 ,  B29C65/14 ,  H01J37/32 ,  G03F7/038 ,  G03F7/00 ,  B81C1/00

Abstract: Imprinted substrates are often used to produce miniaturized devices for use in electrical, optic and biochemical applications. Imprinting techniques, such as nanoimprinting lithography, may leave residues in the surface of substrates that affect bonding and decrease the quality of the produced devices. An imprinted substrate with residue-free region, or regions with a reduced amount of residue for improved bonding quality is introduced. Methods to produce imprinted substrates without residues from the imprinting process are also introduced. Methods include physical exclusion methods, selective etching methods and energy application methods. These methods may produce residue-free regions in the surface of the substrate that can be used to produce higher strength bonding.

公开(公告)号：US20200009525A1

公开(公告)日：2020-01-09

申请号：US16482592

申请日：2018-01-31

Applicant: Illumina, Inc.

Inventor： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC: B01J19/00 ,  G06T7/00 ,  C12Q1/6837 ,  G01N21/64

Abstract: Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include an “always on” type that respond to multiple frequencies of radiation used during processing and detection so as to return signals during successive cycles of imaging.

公开(公告)号：US20180073065A1

公开(公告)日：2018-03-15

申请号：US15187910

申请日：2014-12-23

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Bala Murali Venkatesan ,  Hui Han ,  Sang Ryul Park

IPC: C12Q1/6844 ,  B01J19/00 ,  C40B50/18 ,  B01L3/00 ,  C07K1/04 ,  C12Q1/6813 ,  C12Q1/6825 ,  C12Q1/6837

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  B01J19/0093 ,  B01L3/502707 ,  B01L3/5085 ,  B82Y30/00 ,  C07K1/045 ,  C07K1/047 ,  C12N15/09 ,  C12N15/1093 ,  C12Q1/6813 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B50/18 ,  C12Q2563/107 ,  C12Q2565/628

Abstract: Structured substrate including (a) a plurality of nanoparticles distributed on a solid support, (b) a gel material forming a layer in association with the plurality of nanoparticles, and (c) a library of target nucleic acids in the gel material.

公开(公告)号：US12172156B2

公开(公告)日：2024-12-24

申请号：US18233289

申请日：2023-08-11

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01L3/00 ,  B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US11896944B2

公开(公告)日：2024-02-13

申请号：US16482592

申请日：2018-01-31

Applicant: Illumina, Inc.

Inventor： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC: C12Q1/6837 ,  B01J19/00 ,  G01N21/64 ,  G06T7/00

CPC classification number: B01J19/0046 ,  C12Q1/6837 ,  G01N21/6456 ,  G06T7/0012 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00722

Abstract: Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include an “always on” type that respond to multiple frequencies of radiation used during processing and detection so as to return signals during successive cycles of imaging.

公开(公告)号：US20230381733A1

公开(公告)日：2023-11-30

申请号：US18233289

申请日：2023-08-11

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  B01L3/00 ,  C40B50/18

CPC classification number: B01J19/0046 ,  C12N15/1065 ,  C40B40/08 ,  C08L37/00 ,  C40B50/18 ,  B01L3/502707 ,  B01L3/5027

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US11779897B2

公开(公告)日：2023-10-10

申请号：US17515511

申请日：2021-10-31

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  B01L3/00 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

CPC classification number: B01J19/0046 ,  B01L3/5027 ,  B01L3/502707 ,  C12N15/1065 ,  C40B40/08 ,  C40B50/18 ,  B01J2219/00286 ,  B01J2219/00608 ,  B01L2300/0819 ,  B01L2300/0877 ,  C08L37/00 ,  G01N2021/058

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.