公开(公告)号：US08105824B2

公开(公告)日：2012-01-31

申请号：US12987787

申请日：2011-01-10

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。

公开(公告)号：US07837946B2

公开(公告)日：2010-11-23

申请号：US12291319

申请日：2008-11-06

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US20080108063A1

公开(公告)日：2008-05-08

申请号：US11739660

申请日：2007-04-24

Applicant: Michael Lucero ,  Marc Unger

Inventor： Michael Lucero ,  Marc Unger

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/118 ,  G01N33/53 ,  G01N33/6845 ,  G01N2333/435 ,  G01N2458/10

Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.

Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。

公开(公告)号：US20070196912A1

公开(公告)日：2007-08-23

申请号：US11740735

申请日：2007-04-26

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: C12M1/34

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。

公开(公告)号：US20070004031A1

公开(公告)日：2007-01-04

申请号：US11531155

申请日：2006-09-12

Applicant: Marc Unger ,  Ian Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12M1/34

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US20060118895A1

公开(公告)日：2006-06-08

申请号：US11316282

申请日：2005-12-21

Applicant: Marc Unger

Inventor： Marc Unger

IPC: H01L29/84 ,  H01L21/00

CPC classification number: H01L21/00 ,  B01L3/0241 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0053 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008

Abstract: A valve structure comprises an elastomeric block formed with first and second microfabricated recesses separated by a membrane portion of the elastomeric block. The valve is actuated by positioning a compliant electrode on a first side of the first recess proximate to and in physical communication with the membrane. Where the valve is to be electrostatically actuated, a second electrode is positioned on a second side of the first recess opposite the first side. Application of a potential difference across the electrodes causes the compliant electrode and the membrane to be attracted into the flow channel. Where the valve is to be electrostrictively actuated, a second electrode is positioned on the same side of the recess as the compliant electrode. Application of a potential difference across the electrodes causes the electrodes to be attracted such that elastomer membrane portion material between them is compressed and bows into the flow channel. Either of the electrostrictively or the electrostatically-actuated valve structures may include an electrically-conducting fluid in the second recess to serve as the compliant electrode.

公开(公告)号：US20060093836A1

公开(公告)日：2006-05-04

申请号：US11304316

申请日：2005-12-14

Applicant: Jiang Huang ,  Shoujun Xiao ,  Marc Unger

Inventor： Jiang Huang ,  Shoujun Xiao ,  Marc Unger

IPC: B32B9/04 ,  B32B25/20

CPC classification number: B32B3/26 ,  B01J20/285 ,  B01J20/286 ,  B01J20/327 ,  B01J20/3272 ,  B01J2220/54 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/16 ,  B01L2300/163 ,  C08F257/02 ,  C08F263/04 ,  C08F265/04 ,  C08F279/02 ,  C08F287/00 ,  C08F291/00 ,  C08J5/12 ,  C08J7/12 ,  F04B43/043 ,  Y10T428/139 ,  Y10T428/1393 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/249953 ,  Y10T428/249958 ,  Y10T428/249978 ,  Y10T428/31663 ,  Y10T428/31667

Abstract: The present invention is directed to a surface modified polymer comprising a surface which is covalently bonded to a surface modifying compound. Formation of the covalent bond between the polymer and the surface modifying compound is achieved by a reaction between an intrinsic functional group that is present in the polymer and the functional group of the surface modifying compound. By using a polymer having an intrinsic functional group, a separate surface activation step is avoided.

公开(公告)号：US20050214173A1

公开(公告)日：2005-09-29

申请号：US11058106

申请日：2005-02-14

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转薄膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。

公开(公告)号：US06911345B2

公开(公告)日：2005-06-28

申请号：US09908830

申请日：2001-07-18

Applicant: Stephen Quake ,  Wayne Volkmuth ,  Marc Unger

Inventor： Stephen Quake ,  Wayne Volkmuth ,  Marc Unger

IPC: B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  C12Q1/68 ,  F04B43/04 ,  F15C5/00 ,  F16K99/00 ,  G01N33/549

CPC classification number: C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6869 ,  C12Q1/6874 ,  F04B43/043 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  G01N33/549 ,  C12Q2535/125 ,  C12Q2565/629 ,  C12Q2533/101

Abstract: The present invention provides an apparatus for analyzing the sequences of polynucleotides. The apparatus comprises (a) flow cell which has at least one microfabricated multilayer elastomeric synthesis channel; and (b) an inlet port and an outlet port. The inlet port and outlet ports are in fluid communication with the flow cell for flowing fluids into and through the flow cell.

Abstract translation: 本发明提供了一种用于分析多核苷酸序列的装置。 该装置包括（a）具有至少一个微制造的多层弹性体合成通道的流动池; 和（b）入口和出口。 入口端口和出口端口与流动池流体连通，用于将流体流入和流过流动池。

公开(公告)号：US20050019792A1

公开(公告)日：2005-01-27

申请号：US10819088

申请日：2004-04-05

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Nassef ,  Geoffrey Facer

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Nassef ,  Geoffrey Facer

IPC: C12M1/34 ,  C12Q1/68

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。