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公开(公告)号：US20110189678A1
公开(公告)日：2011-08-04
申请号：US12859176
申请日：2010-08-18
Applicant: Lincoln McBride , Michael Lucero , Marc Unger , Hany Ramez Nassef , Geoffrey Facer
Inventor： Lincoln McBride , Michael Lucero , Marc Unger , Hany Ramez Nassef , Geoffrey Facer
CPC classification number: B01L3/00 , B01F13/0059 , B01J2219/00337 , B01J2219/00353 , B01J2219/00403 , B01J2219/00495 , B01J2219/00585 , B01J2219/0059 , B01J2219/0072 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/50851 , B01L2300/0627 , B01L2300/0883 , B01L2300/123 , B01L2400/06 , C12Q1/6844 , C40B60/08 , C40B60/14 , G01N35/00029 , G01N35/0098 , G01N2035/00366 , Y10T436/25 , C12Q2565/501
Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
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公开(公告)号：US07749737B2
公开(公告)日：2010-07-06
申请号：US11929436
申请日：2007-10-30
Applicant: Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
Inventor： Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
Abstract translation: 一种用于执行反应矩阵的M次.N基质微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US07666361B2
公开(公告)日：2010-02-23
申请号：US10818642
申请日：2004-04-05
Applicant: Lincoln McBride , Marc Unger , Michael Lucero , Hany Ramez Nassef , Geoff Facer , Yong Yi
Inventor： Lincoln McBride , Marc Unger , Michael Lucero , Hany Ramez Nassef , Geoff Facer , Yong Yi
IPC: B01L3/00
CPC classification number: B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US20090142236A1
公开(公告)日：2009-06-04
申请号：US11929660
申请日：2007-10-30
Applicant: Marc Unger , Jiang Huang , Emerson Quan
Inventor： Marc Unger , Jiang Huang , Emerson Quan
IPC: B01J19/00
CPC classification number: B01L3/00 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
Abstract: An M.times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
Abstract translation: 一种用于执行反应矩阵的M.times.N矩阵微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US07476363B2
公开(公告)日：2009-01-13
申请号：US10837885
申请日：2004-05-02
Applicant: Marc Unger , Jiang Huang , Emerson Quan
Inventor： Marc Unger , Jiang Huang , Emerson Quan
IPC: B01L3/00
CPC classification number: B01L3/00 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
Abstract translation: 一种用于执行反应矩阵的MxN矩阵微流体装置，所述装置具有多个反应池，其通过形成在所述装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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Patent Agency Ranking
公开(公告)号：US20050129581A1
公开(公告)日：2005-06-16
申请号：US10818642
申请日：2004-04-05
Applicant: Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
Inventor： Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
CPC classification number: B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US20050112882A1
公开(公告)日：2005-05-26
申请号：US10945737
申请日：2004-09-20
Applicant: Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake
Inventor： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake
IPC: G03F7/20 , B01L3/00 , B81B1/00 , B81B3/00 , B81B7/00 , B81C1/00 , B81C99/00 , C12Q1/68 , F04B17/00 , F04B35/04 , F04B43/04 , F15C5/00 , F16K7/00 , F16K17/02 , F16K99/00 , H01L21/302 , H01L21/461
CPC classification number: B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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公开(公告)号：US09150913B2
公开(公告)日：2015-10-06
申请号：US13548068
申请日：2012-07-12
Applicant: Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
Inventor： Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
Abstract translation: 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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9.发明授权Methods for detection and quantification of nucleic acid or protein targets in a sample 有权检测和定量样品中核酸或蛋白质靶标的方法公开(公告)号：US08828661B2
公开(公告)日：2014-09-09
申请号：US11739660
申请日：2007-04-24
Applicant: Michael Lucero , Marc Unger
Inventor： Michael Lucero , Marc Unger
CPC classification number: C12Q1/686 , C12Q1/6804 , C12Q1/6813 , C12Q1/6876 , C12Q2600/118 , G01N33/53 , G01N33/6845 , G01N2333/435 , G01N2458/10
Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.
Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。
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公开(公告)号：US08247178B2
公开(公告)日：2012-08-21
申请号：US12579347
申请日：2009-10-14
Applicant: Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
Inventor： Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
Abstract translation: 一种用于执行反应矩阵的M×N矩阵微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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