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    Computer-aided probability base calling for arrays of nucleic acid probes on chips
    13.
    发明授权
    Computer-aided probability base calling for arrays of nucleic acid probes on chips 有权
    计算机辅助概率库，要求芯片上的核酸探针阵列

    公开(公告)号：US06228593B1

    公开(公告)日：2001-05-08

    申请号：US09483190

    申请日：2000-01-14

    申请人： Robert J. Lipshutz ,  Michael G. Walker

    发明人： Robert J. Lipshutz ,  Michael G. Walker

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/68 ,  G06F19/20 ,  Y10S435/973

    摘要： A computer system for analyzing nucleic acid sequences is provided. The computer system is used to calculate probabilities for determining unknown bases by analyzing the fluorescence intensities of hybridized nucleic acid probes on biological chips. Additionally, information from multiple experiments is utilized to improve the accuracy of calling unknown bases.

    摘要翻译： 提供了用于分析核酸序列的计算机系统。 计算机系统用于通过分析生物芯片上杂交的核酸探针的荧光强度来计算用于确定未知碱基的概率。 另外，利用来自多个实验的信息来提高呼叫未知基数的准确性。

    Methods of Labeling Cells, Labeled Cells, and uses Thereof
    14.
    发明申请
    Methods of Labeling Cells, Labeled Cells, and uses Thereof 审中-公开
    标记细胞的方法，标记细胞及其用途

    公开(公告)号：US20120178081A1

    公开(公告)日：2012-07-12

    申请号：US13342912

    申请日：2012-01-03

    申请人： Quan N. Nguyen ,  Robert J. Lipshutz ,  Yunqing Ma

    发明人： Quan N. Nguyen ,  Robert J. Lipshutz ,  Yunqing Ma

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54313

    摘要： Methods of detecting nucleic acids, proteins and cells including methods of detecting two or more nucleic acids, proteins and cells in multiplex bDNA assays, are provided. Assays may be conducted at least in vitro, in vivo, in cellulo, and in situ. Nucleic acids are detected, through cooperative hybridization that results in specific association of a label probe system with target nucleic acids. Embodiments are directed to concurrent detection of one or more nucleic acids and/or one or more proteins. The detected proteins may be intracellular or external markers on the surface of the cell. Detection of protein components is accomplished by use of specific antibodies and a label probe system and/or coated microparticles which bind to the outside surface of specific cells and contain specific probes that can be detected using the same label probe system. Compositions, kits, and systems related to the methods are also described.

    摘要翻译： 提供了检测核酸，蛋白质和细胞的方法，包括在多重bDNA测定中检测两种或更多种核酸，蛋白质和细胞的方法。 测定可至少在体外，体内，纤维素和原位进行。 通过协调杂交检测核酸，其导致标记探针系统与靶核酸的特异性结合。 实施方案涉及同时检测一种或多种核酸和/或一种或多种蛋白质。 检测到的蛋白质可以是细胞表面上的细胞内或外部标记。 通过使用特异性抗体和标记探针系统和/或结合特定细胞的外表面并包含可以使用相同标记探针系统检测的特异性探针的包被的微粒来实现蛋白质组分的检测。 还描述了与方法相关的组合物，试剂盒和系统。

    Complexity management and analysis of genomic DNA
    17.
    发明授权
    Complexity management and analysis of genomic DNA 有权
    基因组DNA的复杂性管理与分析

    公开(公告)号：US06361947B1

    公开(公告)日：2002-03-26

    申请号：US09428350

    申请日：1999-10-27

    申请人： Shoulian Dong ,  Robert J. Lipshutz ,  David J. Lockhart

    发明人： Shoulian Dong ,  Robert J. Lipshutz ,  David J. Lockhart

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q2600/156 ,  Y10S977/924 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2521/313 ,  C12Q2563/143

    摘要： The present invention provides for novel methods of sample preparation and analysis involving reproducibly reducing the complexity of a nucleic sample. The invention further provides for analysis of the above sample by hybridization to an array which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism. The invention further provides for novel methods of using a computer system to model enzymatic reactions in order to determine experimental conditions before conducting actual experiments.

    摘要翻译： 本发明提供了重复地降低核酸样品的复杂性的样品制备和分析的新方法。 本发明进一步提供了通过与阵列的杂交来分析上述样品，所述阵列可被特别设计成询问特定特征的期望片段，例如多态性的存在或不存在。 本发明进一步提供了使用计算机系统来模拟酶反应以便在进行实际实验之前确定实验条件的新方法。