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公开(公告)号：US20170065979A1
公开(公告)日：2017-03-09
申请号：US15357840
申请日：2016-11-21
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Christopher F. Kelly , Donald A. Masquelier
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01L3/0275 , B01L3/502715 , B01L3/502723 , B01L7/52 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2400/0487 , C12Q1/6806 , G01N35/085 , G01N35/1065 , G01N2035/00148 , G01N2035/1034 , Y10T29/494
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
Abstract translation: 一种用于产生适合于基于液滴的测定的液滴的方法和装置的系统。 所公开的系统可以包括通过将含水样品的流体与背景乳液流体（例如油）并入而形成含有样品的液滴的乳液，其被配置成通过将含水样品的流体与背景乳液流体例如油混合而构成用于形成含样品的液滴的单件或多件式液滴产生组件 在背景液中。 在一些情况下，所公开的系统可以包括被配置成将含样品的液滴输送到出口区域的通道或其它合适的机制，从而可以执行随后的测定步骤。
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公开(公告)号：US20170056884A1
公开(公告)日：2017-03-02
申请号：US15351331
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
Abstract translation: 用于形成和浓缩乳液的系统，包括方法，装置和试剂盒。 一个示例性系统可以包括一个装置，该装置包括一个样本阱，其被配置成接收含有样品的流体，一个连续相阱，被配置成接收连续相流体，一个液滴阱以及一个使阱连接的通道网络。 该系统还可以包括被配置为可操作地接收该装置并且产生（i）第一压差以产生在液滴阱中收集的乳液的仪器，和（ii）第二压力差以减小连续相流体的体积分数 在乳液中，在乳液已经收集在液滴中之后，通过相对于含有样品的液滴从液滴中选择性驱动连续相流体。
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公开(公告)号：US09399215B2
公开(公告)日：2016-07-26
申请号：US13863231
申请日：2013-04-15
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Thomas H. Cauley, III , Luc Bousse , Jonathan Petersen , Kevin D. Ness , Steven Romine
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/502746 , B01L3/502784 , B01L2200/027 , B01L2200/0673 , B01L2300/161 , B01L2400/0406 , B01L2400/086 , B01L2400/088 , B32B38/0012 , G01N1/00 , Y10T156/10 , Y10T156/1002
Abstract: Sample holding system, including methods and apparatus, including a holder defining a well having a wicking promoter that encourages flow of a sample to the bottom of the well. Methods of making and using the holder are also disclosed.
Abstract translation: 样品保持系统，包括方法和装置，包括限定具有促进样品流到井的底部的芯吸促进剂的阱的保持器。 还公开了制造和使用保持器的方法。
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公开(公告)号：US20160076099A1
公开(公告)日：2016-03-17
申请号：US14848311
申请日：2015-09-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q1/683 , C12Q1/6858 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.
Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使得样品中核酸的拷贝数估计可以进行。 本文还提供了用于确定样品中靶核酸的两个或多个拷贝（例如两个或多个拷贝是在同一染色体上还是在不同染色体上）或用于定相等位基因的连接的方法，组合物和试剂盒。
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公开(公告)号：US20140200164A1
公开(公告)日：2014-07-17
申请号：US14159410
申请日：2014-01-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Anthony J. Makarewicz, JR. , Amy L. Hiddessen , Kevin D. Ness , Donald. A. Masquelier , Billy W. Colston, JR. , Benjamin J. Hindson , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: C12Q1/68
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods and apparatus, for detection of spaced droplets.
Abstract translation: 用于检测间隔液滴的系统，包括方法和装置。
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Patent Agency Ranking
公开(公告)号：US20130269452A1
公开(公告)日：2013-10-17
申请号：US13863231
申请日：2013-04-15
Applicant: BIO-RAD LABORATORIES, INC.
Inventor： Thomas H. Cauley III , Luc Bousse , Jon Petersen , Kevin D. Ness , Steven Romine
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/502746 , B01L3/502784 , B01L2200/027 , B01L2200/0673 , B01L2300/161 , B01L2400/0406 , B01L2400/086 , B01L2400/088 , B32B38/0012 , G01N1/00 , Y10T156/10 , Y10T156/1002
Abstract: Sample holding system, including methods and apparatus, including a holder defining a well having a wicking promoter that encourages flow of a sample to the bottom of the well. Methods of making and using the holder are also disclosed.
Abstract translation: 样品保持系统，包括方法和装置，包括限定具有促进样品流到井的底部的芯吸促进剂的阱的保持器。 还公开了制造和使用保持器的方法。
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公开(公告)号：US12103005B2
公开(公告)日：2024-10-01
申请号：US16995555
申请日：2020-08-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Christopher F. Kelly , Donald A. Masquelier
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01L3/02 , B01L7/00 , C12Q1/6806 , G01N35/00 , G01N35/08 , G01N35/10
CPC classification number: B01L3/502784 , B01F23/41 , B01F33/3011 , B01L3/502715 , B01L3/502723 , C12Q1/6806 , G01N35/085 , B01L3/0275 , B01L7/52 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2400/0487 , G01N2035/00148 , G01N2035/1034 , G01N35/1065 , Y10T29/494
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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公开(公告)号：US11633739B2
公开(公告)日：2023-04-25
申请号：US17137274
申请日：2020-12-29
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Donald A. Masquelier , Billy W. Colston, Jr. , Benjamin J. Hindson
IPC: B01L3/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F23/41 , B01F33/3011 , B01F35/10 , C12Q1/686 , G01N21/64 , B29C45/00 , G01N21/49 , B01F101/23 , B29L31/00
Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.
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公开(公告)号：US20220008914A1
公开(公告)日：2022-01-13
申请号：US17486667
申请日：2021-09-27
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US11130128B2
公开(公告)日：2021-09-28
申请号：US16667811
申请日：2019-10-29
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Donald A. Masquelier , Billy W. Colston, Jr. , Benjamin J. Hindson
IPC: B01L3/00 , B01F3/08 , C12Q1/686 , B01F5/00 , G01N35/08 , B01F5/02 , B01F13/00 , B01F13/10 , B01L9/00
Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.
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