-
公开(公告)号：US5968740A
公开(公告)日：1999-10-19
申请号：US811829
申请日：1997-03-05
CPC分类号： C12Q1/6837 , B82Y30/00 , C12Q1/6874 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , C40B40/06
摘要： Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.
摘要翻译： 用于核酸杂交和用于确定核酸序列的装置和技术。 核酸序列通过技术，优选高分辨率，光导技术形成。 通过例如落射荧光显微镜测定靶核酸的杂交位置。 提出了设备和技术，以通过这种合成和检测技术更有效和更快地确定目标核酸的序列。
-
32.发明授权Computer-aided engineering system for design of sequence arrays and lithographic masks 失效用于设计序列阵列和光刻掩模的计算机辅助工程系统
公开(公告)号：US5593839A
公开(公告)日：1997-01-14
申请号：US460411
申请日：1995-06-02
IPC分类号： B01J19/00 , C07B61/00 , C07K1/04 , C40B40/06 , C40B60/14 , G03F1/00 , C12Q1/68 , C07H21/04 , G03F9/00
CPC分类号： B82Y30/00 , B01J19/0046 , C07K1/04 , C07K1/045 , B01J2219/00432 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00693 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： An improved set of computer tools for forming arrays. According to one aspect of the invention, a computer system is used to select probes and design the layout of an array of DNA or other polymers with certain beneficial characteristics. According to another aspect of the invention, a computer system uses chip design files to design and/or generate lithographic masks.
摘要翻译： 一组改进的用于形成阵列的计算机工具。 根据本发明的一个方面，计算机系统用于选择探针并设计具有某些有益特征的DNA或其它聚合物阵列的布局。 根据本发明的另一方面，计算机系统使用芯片设计文件来设计和/或生成光刻掩模。
-
公开(公告)号：US20110028352A1
公开(公告)日：2011-02-03
申请号：US12847570
申请日：2010-07-30
CPC分类号： C40B60/12 , B01J2219/00479 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6834
摘要： A method, device and system for hybridizing a target oligonucleotide to at least one array comprising a plurality of mixing beads are provided. A target solution is mixed by agitating the mixing beads while the target oligonucleotides are hybridizing to the complementary probes on the array. In another embodiment, a permeable barrier contains the mixing beads, thereby preventing them from contacting the array surface.
摘要翻译： 提供了将目标寡核苷酸与包括多个混合珠的至少一个阵列杂交的方法，装置和系统。 通过搅拌混合珠混合目标溶液，同时靶寡核苷酸与阵列上的互补探针杂交。 在另一个实施方案中，可渗透屏障包含混合珠，从而防止它们与阵列表面接触。
-
34.发明授权Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping 有权捕获与基因组文库映射的类型-Ill限制位点相邻的序列公开(公告)号：US07662559B2
公开(公告)日：2010-02-16
申请号：US10942479
申请日：2004-09-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
-
公开(公告)号：US07267966B2
公开(公告)日：2007-09-11
申请号：US09904039
申请日：2001-07-12
CPC分类号： C12Q1/6837 , C12Q1/6855 , C12Q1/6858 , C12Q2600/156 , Y10S977/924 , C12Q2531/113 , C12Q2525/191 , C12Q2521/313 , C12Q2563/143
摘要： The present invention provides for novel methods of sample preparation and analysis involving reproducibly reducing the complexity of a nucleic sample. The invention further provides for analysis of the above sample by hybridization to an array which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism. The invention further provides for novel methods of using a computer system to model enzymatic reactions in order to determine experimental conditions before conducting actual experiments.
摘要翻译： 本发明提供了重复地降低核酸样品的复杂性的样品制备和分析的新方法。 本发明进一步提供了通过与阵列的杂交来分析上述样品，所述阵列可被特别设计成询问特定特征的期望片段，例如多态性的存在或不存在。 本发明进一步提供了使用计算机系统来模拟酶反应以便在进行实际实验之前确定实验条件的新方法。
-
公开(公告)号：US06489146B2
公开(公告)日：2002-12-03
申请号：US09245802
申请日：1999-02-05
IPC分类号： C12P1934
CPC分类号： C12N15/10 , C12N15/64 , C12N15/66 , C12Q1/6853 , C12Q1/686 , C12Q2537/143 , C12Q2531/125 , C12Q2525/307 , C12Q2525/151 , C12Q2525/155
摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof and for assembling large polynucleotides from component polynucleotides, each involving generating concatemers formed by PCR amplification of overlapping fragments.
摘要翻译： 本发明涉及用于扩增和检测核酸或其混合物中包含的任何靶核酸序列并用于从组分多核苷酸组装大多核苷酸的方法，每个涉及产生通过重叠片段的PCR扩增形成的连接体。
-
37.发明授权Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same 失效用于分析生物转化基因的核酸探针阵列及其使用方法公开(公告)号：US06309823B1
公开(公告)日：2001-10-30
申请号：US08778794
申请日：1997-01-03
申请人： Maureen T. Cronin , Charles G Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles G Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
IPC分类号： C12Q168
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , C12Q1/6876 , C12Q1/6883 , C12Q2600/106 , C12Q2600/156 , C40B40/06 , C40B60/14 , C12Q2565/513
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequece of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.
摘要翻译： 本发明提供了固定化探针的阵列，以及采用该阵列的方法，用于检测生物转化基因如细胞色素P450中的突变。 例如，一个这样的阵列包括四个探针组。 第一探针组包含多个探针，每个探针包含与来自生物转化基因的参考序列的亚序列完全互补的至少三个核苷酸的片段，该片段包含与参考文献中的相应核苷酸互补的至少一个询问位置 序列。 第二，第三和第四探针组各自包括在第一探针组中的每个探针的相应探针。 第二，第三和第四探针组中的探针与包括来自第一探针组的相应探针或其至少包含至少一个询问位置的至少三个核苷酸的子序列的序列相同，除了至少一个询问 位置由来自四个探针组的四个相应探针中的每一个中的不同核苷酸占据。
-
公开(公告)号：US06280950B1
公开(公告)日：2001-08-28
申请号：US09429521
申请日：1999-10-28
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequences; b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.
摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中， 所述寡核苷酸的密度大于每平方厘米约60个不同的寡核苷酸，并且所有所述不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列; b）扩增所述多重寡核苷酸以提供扩增核酸的库; 和c）将核酸池连接到固体支持物上。
-
公开(公告)号：US06268152B1
公开(公告)日：2001-07-31
申请号：US09543789
申请日：2000-04-06
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q2600/156 , C40B40/06 , C40B60/14 , G06F19/20 , C12Q2565/513 , C12Q2525/186 , C12Q2565/501 , C12Q2565/518 , C12Q2525/161 , C12Q2525/204
摘要： Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.
摘要翻译： 用于核酸杂交和用于确定核酸序列的装置和技术。 核酸序列通过技术，优选高分辨率，光导技术形成。 通过例如落射荧光显微镜测定靶核酸的杂交位置。 提出了设备和技术，以通过这种合成和检测技术更有效和更快地确定目标核酸的序列。
-
公开(公告)号：US06197595B1
公开(公告)日：2001-03-06
申请号：US09294700
申请日：1999-04-19
IPC分类号： G01N110
CPC分类号： B01F15/0201 , B01F11/0071 , B01F11/0266 , B01F13/005 , B01F13/0059 , B01F13/08 , B01F15/0238 , B01F2215/0037 , B01F2215/0073 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L3/502784 , B01L7/52 , B01L9/527 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2300/087 , B01L2300/0883 , B01L2300/16 , B01L2400/0415 , B01L2400/0421 , B01L2400/0487 , B01L2400/049 , B01L2400/084 , B01L2400/086 , G01N35/1095 , G01N2035/00158 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.
摘要翻译： 本发明提供了一种小型化的综合核酸诊断装置和系统。 本发明的装置通常能够结合一个或多个样品分析操作来执行一个或多个样品采集和制备操作。 例如，该设备可以在单个集成单元中集成样品采集和存储，样品制备和样品分析中涉及的几个或全部操作。 该装置可用于各种应用，尤其是基于核酸的诊断应用和从头测序应用。
-
-
-
-
-
-
-
-
-
搜索结果
56 条记录 (耗时 0.04 秒)
