利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

102 条记录 (耗时 0.046 秒)

    Methods and Systems for Molecular Fingerprinting
    48.
    发明申请
    Methods and Systems for Molecular Fingerprinting 审中-公开
    分子指纹图谱的方法与系统

    公开(公告)号：US20110177499A1

    公开(公告)日：2011-07-21

    申请号：US13079753

    申请日：2011-04-04

    申请人： Stephen R. Quake ,  Hou-Pu Chou

    发明人： Stephen R. Quake ,  Hou-Pu Chou

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0484 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0633 ,  C12Q1/6816 ,  C12Q1/683 ,  G01N2015/149 ,  C12Q2565/629

    摘要： This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.

    摘要翻译： 本发明一般涉及分子指纹图谱的方法。 该方法可用于法医鉴定（例如DNA指纹图谱，特别是VNTR），细菌分型和人/动物病原体诊断。 更具体地，可以按照限制性片段长度多态性分析多核苷酸的微制造装置中的大小来评估或分类分子，例如多核苷酸（例如DNA）。 在根据本发明的微制造装置中，通过测量例如与多核苷酸片段相关的可光学检测（例如，荧光）报告基因的信号，可以使用相对小的样品快速和精确地分型DNA片段或其他分子。

    Microfluidic devices and methods of use
    49.
    发明授权
    Microfluidic devices and methods of use 有权
    微流体装置及使用方法

    公开(公告)号：US07258774B2

    公开(公告)日：2007-08-21

    申请号：US09970122

    申请日：2001-10-02

    申请人： Hou-Pu Chou ,  Anne Y. Fu ,  Stephen R. Quake

    发明人： Hou-Pu Chou ,  Anne Y. Fu ,  Stephen R. Quake

    IPC分类号： F04B43/00

    CPC分类号： F04B43/00 ,  A61M2206/22 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  F04B11/00 ,  F04B19/006 ,  F04B43/043 ,  G01N30/6095 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2030/205 ,  G01N2030/322 ,  Y10T436/2575

    摘要： A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.

    摘要翻译： 微流体装置包括泵，阀和流体振荡阻尼器。 在用于排序的装置中，实体通过泵通过检测区域的流动通道流到结。 基于在检测区域中确定的实体的身份，致动位于连接处的流动通道的相对分支上的废物或收集阀，从而将该实体路由到废物池或收集池。 阻尼器结构可以位于泵和接头之间。 阻尼器由于泵的操作而减小了流动通道中的振荡压力的振幅，从而减少了分离过程中实体的速度振荡。 微流体装置可以形成为弹性体材料块，弹性体材料的薄膜可偏转到流动通道中以提供泵或阀的功能。