公开(公告)号：US11823809B2

公开(公告)日：2023-11-21

申请号：US17368078

申请日：2021-07-06

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Jiangbo Yu ,  Yu Rong ,  Changfeng Wu

IPC: H01B1/18 ,  G02F1/00 ,  C07K14/00 ,  C07K16/00 ,  C08K3/36 ,  C08L83/04 ,  G02F1/015

CPC classification number: H01B1/18 ,  C07K14/00 ,  C07K16/00 ,  C08K3/36 ,  C08L83/04 ,  G02F1/015 ,  C08K2201/001 ,  C08L2203/20

Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.

公开(公告)号：US11808767B2

公开(公告)日：2023-11-07

申请号：US17932687

申请日：2022-09-16

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Wyatt Nelson ,  Perry G. Schiro

IPC: G01N33/574 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/53 ,  G01N35/10 ,  G01N35/00 ,  G01N15/10

CPC classification number: G01N33/57492 ,  B01L3/502761 ,  B01L3/561 ,  B01L3/567 ,  G01N15/0612 ,  G01N15/0656 ,  G01N15/1456 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/49 ,  G01N33/5304 ,  B01L3/502738 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0633 ,  B01L2400/086 ,  G01N15/0618 ,  G01N35/0098 ,  G01N2015/0681 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00237 ,  G01N2035/00356 ,  G01N2035/1034

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US10794925B2

公开(公告)日：2020-10-06

申请号：US15741462

申请日：2016-07-07

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Bryant S. Fujimoto ,  Jason E. Kreutz

IPC: B01L7/00 ,  C12Q1/686 ,  G01N35/10 ,  G01N21/64 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N15/14 ,  B01L3/00 ,  B01L9/00 ,  G01N15/10

Abstract: Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.

公开(公告)号：US10770197B2

公开(公告)日：2020-09-08

申请号：US16309795

申请日：2017-06-13

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Jiangbo Yu ,  Yu Rong ,  Changfeng Wu

IPC: H01B1/00 ,  C07K14/00 ,  H01B1/18 ,  C07K16/00 ,  C08K3/36 ,  C08L83/04

Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.

公开(公告)号：US10768180B2

公开(公告)日：2020-09-08

申请号：US16041569

申请日：2018-07-20

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Yuhui Jin ,  Fangmao Ye ,  Changfeng Wu ,  Yang-Hsiang Chan

IPC: G01N33/58 ,  H01L51/00 ,  B82Y40/00 ,  A61K49/00 ,  H01L51/50 ,  B82Y15/00 ,  B82Y5/00

Abstract: Polymer nanoparticles and related methods are provided. The polymer particles can include polymer dots having a coating including a polyelectrolyte polymer. Methods of making and using the polymer nanoparticles are also provided.

公开(公告)号：US10514381B2

公开(公告)日：2019-12-24

申请号：US14774971

申请日：2014-03-14

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Wei Sun ,  Jiangbo Yu ,  Changfeng Wu ,  Fangmao Ye

IPC: G01N33/58 ,  C09K11/02 ,  G01N33/543 ,  C08G61/02 ,  C08G73/00 ,  C08G75/32 ,  G01N33/533

Abstract: Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

公开(公告)号：US20190217289A1

公开(公告)日：2019-07-18

申请号：US16366956

申请日：2019-03-27

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Dawn E. Cohen ,  Gavin D.M. Jeffries

IPC: B01L3/00 ,  G01N35/10 ,  G01N35/08 ,  B01L7/00

CPC classification number: B01L3/502 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2300/165 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0439 ,  B01L2400/0633 ,  G01N35/08 ,  G01N35/10 ,  G01N2035/00356 ,  G01N2035/1032 ,  G01N2035/1034

Abstract: Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements. The minimal fluid interconnects and simple flow geometry makes the device easy to use and implement, economical to fabricate and operate, and robust in its operations.

公开(公告)号：US10236170B2

公开(公告)日：2019-03-19

申请号：US15454558

申请日：2017-03-09

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Dingsheng Liu

IPC: H01J49/16 ,  B05B5/03 ,  H01J49/04 ,  B05B5/025

Abstract: The present disclosure describes devices and methods capable of generating multi¬phase emulsions, including double emulsion droplets in a gas phase. The present disclosure also describes interfaces for coupling a multi-phase emulsion droplet source to an analytical instrument such as a mass spectrometer. The present disclosure further describes methods, systems, and apparatuses for using the devices and interfaces described to perform analysis, including mass spectrometry. The present disclosure also describes methods, systems, and apparatuses for generating and using multi-phase emulsions to perform analysis.

公开(公告)号：US09810693B2

公开(公告)日：2017-11-07

申请号：US13865942

申请日：2013-04-18

Applicant: University of Washington Through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Xuanjun Zhang ,  Jiangbo Yu ,  Fangmao Ye

IPC: G01N33/58 ,  C09K11/06 ,  B82Y30/00 ,  B82Y40/00 ,  H01L51/00 ,  B82Y15/00 ,  C08G61/12 ,  C08J3/11 ,  C09K11/02

CPC classification number: G01N33/588 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G61/126 ,  C08G2261/12 ,  C08G2261/1426 ,  C08G2261/228 ,  C08G2261/3142 ,  C08G2261/3246 ,  C08G2261/522 ,  C08G2261/54 ,  C08G2261/72 ,  C08G2261/94 ,  C08J3/11 ,  C08J2327/22 ,  C09K11/025 ,  C09K11/06 ,  C09K2211/1425 ,  H01L51/0039

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

公开(公告)号：US09733165B2

公开(公告)日：2017-08-15

申请号：US14170381

申请日：2014-01-31

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Jason S. Kuo

IPC: C12Q1/00 ,  G01N1/34 ,  B01L3/00 ,  C12N5/09

CPC classification number: G01N1/34 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N5/0693

Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells.