公开(公告)号：US20030096268A1

公开(公告)日：2003-05-22

申请号：US10191438

申请日：2002-07-08

Inventor： Michael Weiner ,  Said Attiya ,  Hugh Crenshaw ,  Jonathan Rothberg ,  Steve Matson

IPC: C12Q001/68 ,  C12M001/34

CPC classification number: B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00313 ,  B01J2219/00355 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6869 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

Abstract translation: 本发明涉及流体动力学领域。 更具体地，本发明涉及用于以基本上二维阵列并行进行密集包装的独立化学反应的方法和装置。 因此，本发明还着重于将该阵列用于诸如DNA测序，最优选焦磷酸测序和DNA扩增的应用。

公开(公告)号：US20030069411A1

公开(公告)日：2003-04-10

申请号：US10171497

申请日：2002-06-12

Inventor： Thomas M. Brennan

IPC: C07H021/04 ,  C12M001/34 ,  B32B027/12

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/0031 ,  B01J2219/00313 ,  B01J2219/00315 ,  B01J2219/00328 ,  B01J2219/00364 ,  B01J2219/00369 ,  B01J2219/00414 ,  B01J2219/00416 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/00454 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07B2200/11 ,  C07K1/04 ,  C07K1/045 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  G01N35/1072

Abstract: A polymer synthesis apparatus (20) for building a polymer chain including a head assembly (21) having an array of nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having an array of reaction wells (26). A transport mechanism (27) aligns the reaction wells (26) and selected nozzles (22) for deposition of the liquid reagent (24) into selected reaction wells (26). A sliding seal (30) is positioned between the head assembly (21) and the base assembly (25) to form a common chamber (31) enclosing both the reaction well (26) and the nozzles (22) therein. A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. Each reaction well (26) includes an orifice (74) extending into the well (26) which is of a size and dimension to form a capillary liquid seal to retain the reagent solution (76) in the well (26) for polymer chain growth therein. A pressure regulating device (82) is provided for controlling a pressure differential, between a first gas pressure exerted on the reaction well (26) and a second gas pressure exerted on an exit (80) of the orifice, such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled from the well (26) through the orifice (74). A method of synthesis of a polymer chain in a synthesis apparatus (20) is also included.

Abstract translation: 一种用于构建聚合物链的聚合物合成设备（20），其包括具有喷嘴阵列（22）的头组件（21），每个喷嘴连接到液体试剂（24）的容器（23），以及基座组件 ）具有反应孔阵列（26）。 输送机构（27）对准反应孔（26）和选定的喷嘴（22），用于将液体试剂（24）沉积到选定的反应孔（26）中。 滑动密封件（30）定位在头部组件（21）和基座组件（25）之间，以形成在其中包围反应井（26）和喷嘴（22）的公共腔室（31）。 从喷嘴（22）的上游进入公共室（31）的气体入口（70）和在公共室（31）之外的喷嘴（22）的下游的气体出口（71）扫过公共室 （31）试剂发出的有毒烟雾。 每个反应井（26）包括延伸到井（26）中的孔（74），其具有尺寸和尺寸以形成毛细管液体密封，以将试剂溶液（76）保持在井（26）中用于聚合物链增长 其中。 提供压力调节装置（82），用于控制施加在反应井（26）上的第一气体压力和施加在孔口出口（80）上的第二气体压力之间的压力差，使得在压差 超过预定量的试剂溶液（76）通过孔口（74）从孔26排出。 也包括在合成装置（20）中合成聚合物链的方法。

公开(公告)号：US20030055295A1

公开(公告)日：2003-03-20

申请号：US10233720

申请日：2002-09-04

Applicant: General Electric Company

Inventor： William Patrick Flanagan ,  James Lawrence Spivack ,  Cheryl Lynn Sabourin

IPC: C07C039/16

CPC classification number: B01J19/0046 ,  B01J19/0006 ,  B01J2219/00283 ,  B01J2219/00335 ,  B01J2219/00367 ,  B01J2219/00373 ,  B01J2219/00414 ,  B01J2219/00481 ,  B01J2219/00497 ,  B01J2219/00596 ,  B01J2219/0068 ,  B01J2219/00691 ,  B01J2219/0072 ,  C40B60/14

Abstract: Method for producing multiple chemical reactions and for rapid screening of chemicals, catalysts, process conditions and the like is disclosed. The method includes the steps of providing an array of reactor vessels and reactants; loading each reactor vessel with at least one reactant; and allowing the reactions to proceed for a predetermined time interval. A volume increment is withdrawn from each reactor vessel and a volume increment of at least one reactant is added to each reactor vessel in the array. The steps of volume increment withdrawal and addition are repeated after successive time intervals until the reactions reach a substantially steady state. The loading, withdrawal, and addition steps are performed by liquid or solid handling robots. In one embodiment, the volume increment withdrawal occurs before, after, or contemporaneously with the volume increment addition.

公开(公告)号：US20030045758A1

公开(公告)日：2003-03-06

申请号：US10180870

申请日：2002-06-26

Applicant: General Electric Company

Inventor： William Patrick Flanagan ,  Cheryl Lynn Sabourin ,  James Lawrence Spivack

IPC: C07C039/12 ,  C07C039/16

CPC classification number: B01J4/02 ,  B01J19/0006 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00335 ,  B01J2219/00367 ,  B01J2219/00373 ,  B01J2219/00414 ,  B01J2219/00481 ,  B01J2219/00497 ,  B01J2219/00596 ,  B01J2219/0068 ,  B01J2219/00691 ,  B01J2219/0072 ,  C40B60/14

Abstract: A method of producing a chemical reaction is provided. In the practice of one embodiment of the invention, the method includes the steps of providing a reaction vessel and reactants; placing at least one of the reactants in the reaction vessel; and allowing the reaction to proceed for a time interval. A volume increment of at least one of the reactants is withdrawn from the reaction vessel, and a volume increment of at least one of the reactants is added to the reaction vessel. The volume increment withdrawal/addition is repeated after successive time intervals until the reaction reaches a substantially steady state. In various alternative embodiments, the volume increment withdrawal can take place before, after, or contemporaneously with the volume increment addition.

Abstract translation: 提供了生产化学反应的方法。 在本发明的一个实施方案的实践中，该方法包括提供反应容器和反应物的步骤; 将至少一种反应物置于反应容器中; 并允许反应进行一段时间。 将至少一种反应物的体积增量从反应容器中取出，并将至少一种反应物的体积增量加入到反应容器中。 在连续的时间间隔之后重复体积增量提取/添加，直到反应达到基本上稳定的状态。 在各种替代实施例中，体积增量提取可以在体积增量添加之前，之后或同时进行。

公开(公告)号：US06497155B1

公开(公告)日：2002-12-24

申请号：US09247722

申请日：1999-02-09

Applicant: Ilya Feygin ,  Rhett L. Affleck ,  Aleksandr Grinberg ,  Thuc H. Nguyen

Inventor： Ilya Feygin ,  Rhett L. Affleck ,  Aleksandr Grinberg ,  Thuc H. Nguyen

IPC: G01N3510

CPC classification number: G01N35/10 ,  B01J2219/00376 ,  B01J2219/00414 ,  B01J2219/00468 ,  C40B60/14 ,  G01N15/0272 ,  G01N35/1074 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2035/00237 ,  G01N2035/00574 ,  G01N2035/1039 ,  Y10T436/114998 ,  Y10T436/119163

Abstract: In one embodiment, a particle-retrieval device in accordance with the present teachings includes a receiver tube, vacuum-flow providing means and particle-disengaging means. The receiver tube is in fluid communication with the vacuum-flow providing means such that when flow is introduced into the vacuum-flow providing means, a suction or vacuum flow is developed at an end of the receiver tube. The suction causes a particle to adhere to the end of the receiver. A particle is disengaged by discontinuing the suction and, advantageously, by wetting the engaged particle.

Abstract translation: 在一个实施例中，根据本教导的颗粒检索装置包括接收管，真空流提供装置和颗粒分离装置。 接收管与真空流提供装置流体连通，使得当流入真空流提供装置时，吸收管或真空流在接收管的一端形成。 吸力使颗粒粘附到接收器的末端。 通过中断吸力并有利地通过润湿接合的颗粒来分离颗粒。

公开(公告)号：US5711917A

公开(公告)日：1998-01-27

申请号：US665959

申请日：1996-06-17

Applicant: David L. Juranas ,  Donald D. Munn ,  Harry A. Anderson

Inventor： David L. Juranas ,  Donald D. Munn ,  Harry A. Anderson

IPC: B01J19/00 ,  C40B60/14 ,  G01N1/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00306 ,  B01J2219/00308 ,  B01J2219/00335 ,  B01J2219/00364 ,  B01J2219/00373 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00484 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  C40B60/14 ,  G01N35/1079 ,  Y10T436/25375

Abstract: A laboratory reactor apparatus includes a suction tube, at least a portion of which has a constricted inside diameter. The lower portion of the suction tube is sealed to a sintered frit filter medium. The suction tube mounts in a reactor block into a reactor vial for receiving chemical components. An inlet channel with a first seal is formed through the block and conveys chemical component materials to the vial, and a suction channel with a second seal, coaxial with the suction tube, is used to remove resultant chemical compound materials therefrom. A pressurized gas channel containing inert gas is formed through the block to intersect the suction channel and the inlet channel.

Abstract translation: 实验室反应器装置包括吸入管，其至少一部分具有缩径的内径。 吸入管的下部被密封到烧结玻璃料过滤介质上。 吸入管将反应器块安装到用于接收化学组分的反应器小瓶中。 通过该块形成具有第一密封件的入口通道，并将化学成分材料输送到小瓶，并且使用具有与抽吸管同轴的第二密封件的抽吸通道从其中除去所得到的化合物材料。 通过该块形成含有惰性气体的加压气体通道以与吸入通道和入口通道相交。

公开(公告)号：US5496473A

公开(公告)日：1996-03-05

申请号：US313221

申请日：1994-09-30

Applicant: Flora Chow

Inventor： Flora Chow

IPC: B01J19/00 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14 ,  G01N30/06 ,  G01N30/24 ,  G01N30/46 ,  G01N30/82 ,  G01N30/88 ,  B01D15/08

CPC classification number: C07H21/00 ,  B01J19/0046 ,  G01N30/88 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00454 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N30/06 ,  G01N30/24 ,  G01N30/466 ,  G01N30/82

Abstract: An apparatus has been designed that is useful for the automated or semi-automated removal, recovery, deprotection, and/or purification of biopolymers such as oligodeoxyribonucleotides, oligoribonucleotides, oligosaccharides, and peptides from a solid support matrix that is contained in a reaction chamber in the shape of a column.

Abstract translation: 已经设计了一种可用于自动或半自动移除，回收，脱保护和/或纯化生物聚合物如寡脱氧核糖核苷酸，寡核糖核苷酸，寡糖和来自固体支持基质的肽，该固体支持基质包含在反应室中 列的形状。

公开(公告)号：US20180319906A1

公开(公告)日：2018-11-08

申请号：US15773591

申请日：2016-11-15

Applicant: BASF SE

Inventor： Marco Kruger ,  Karl Possemiers ,  Gerald Gruenewald ,  Juergen Freiberg ,  Markus Muehl

IPC: C08F2/01 ,  C08F2/12 ,  C08F20/14 ,  B01J2/16 ,  B01J19/24 ,  B01J4/00

CPC classification number: C08F2/01 ,  B01J2/16 ,  B01J4/002 ,  B01J19/24 ,  B01J19/2405 ,  B01J2219/00414 ,  B01J2219/0072 ,  B01J2219/185 ,  C08F2/12 ,  C08F2/34 ,  C08F20/14

Abstract: The invention relates to an apparatus for producing pulverulent poly(meth)acrylate, comprising a reactor for droplet polymerization having an apparatus for dropletization of a monomer solution for the preparation of the poly(meth)acrylate having holes through which the monomer solution is introduced, an addition point for a gas above the apparatus for dropletization, at least one gas withdrawal point on the circumference of the reactor, a fluidized bed and an apparatus for product discharge from the fluidized bed. The apparatus for product discharge comprises a discharge apparatus, with a backup segment (39) disposed above the discharge apparatus.

公开(公告)号：US09182171B2

公开(公告)日：2015-11-10

申请号：US13121489

申请日：2009-09-29

Applicant: Peter Lea

Inventor： Peter Lea

IPC: F26B7/00 ,  F26B5/12 ,  F26B5/04 ,  G01N35/10

CPC classification number: F26B5/12 ,  B01J2219/00315 ,  B01J2219/00414 ,  B01J2219/00605 ,  B01L2200/025 ,  B01L2400/049 ,  F26B5/04 ,  G01N35/1074

Abstract: The present invention relates to a method and device for the removal of fluids and vapors in containment, from adjacent surfaces including supporting flat surfaces and substrates, such as protein spots printed in arrays and micro-array formats. This method and device removes fluids and vapors under controlled and repeatable conditions, enabling uniform phases for degree of drying incurred by objects, specimen and structures, including tissues, adsorbed particles and biological substrates, while reducing and preventing impact of meniscus phase surface tension forces.

Abstract translation: 本发明涉及一种用于从包含支撑平表面和底物的相邻表面（例如以阵列印刷的蛋白质斑点和微阵列形式）中的流体和蒸汽中除去流体和蒸气的方法和装置。 该方法和装置在受控和可重复的条件下去除流体和蒸汽，使物体，样品和结构（包括组织，吸附的颗粒和生物基底）产生的干燥程度均匀，同时减少和防止弯液面相表面张力的影响。

公开(公告)号：US20150072405A1

公开(公告)日：2015-03-12

申请号：US14372202

申请日：2012-01-19

Applicant: Saburo Ito

Inventor： Saburo Ito

IPC: C12M1/32 ,  B01L3/00

CPC classification number: C12M23/12 ,  B01J2219/00315 ,  B01J2219/00414 ,  B01J2219/00468 ,  B01L3/021 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  G01N35/1011

Abstract: A well plate is formed with a well for holding a subject to be sucked by a suction nozzle on an inner bottom part and storing liquid and, a clearance forming member for forming a clearance to allow the liquid to flow in a state where a tip part of the suction nozzle is inserted into and held in contact with the well is provided in the well. According to the present invention, the clearance enabling the liquid to flow is formed even in the state where the tip part of the suction nozzle is inserted into and held in contact with the well in sucking the subject held in the well by the suction nozzle. The suction nozzle can suck the liquid around through the clearance and the subject held in the well is efficiently sucked through the suction port along the flow of the sucked liquid.

Abstract translation: 阱板形成有用于保持被吸收在内底部上的吸嘴并存储液体的孔，以及用于形成间隙的间隙形成构件，以允许液体在尖端部分 的吸入口被插入并保持与井接触。 根据本发明，即使在抽吸嘴的顶端部分插入并保持与井接触的状态下，也可以形成使液体流动的间隙，从而通过吸嘴吸住保持在井中的被摄体。 吸嘴可以通过间隙吸附液体，并且保持在井中的被摄体沿着吸入液体的流动被有效地吸入吸入口。