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    Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles
    51.
    发明授权
    Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles 失效
    测量微粒的方法，其定量测量方法和测量微粒的仪器

    公开(公告)号：US5308990A

    公开(公告)日：1994-05-03

    申请号：US882954

    申请日：1992-05-14

    申请人： Satoshi Takahashi ,  Daizo Tokinaga ,  Kazunori Okano

    发明人： Satoshi Takahashi ,  Daizo Tokinaga ,  Kazunori Okano

    IPC分类号： F02B75/02 ,  G01N15/14

    CPC分类号： G01N15/1459 ,  F02B2075/027 ,  G01N2015/0092 ,  G01N2015/1486 ,  G01N2021/6439

    摘要： A microparticle measuring method according to the present invention, by which the number of fluorescent microparticles is counted and the fluorescent microparticles are analyzed, includes the steps of introducing fluorescent microparticles into a narrow flow path almost one after another; irradiating the fluorescent microparticles in the narrow flow path with excitation light; detecting a signal pulse produced by detection of a single photon of fluorescence generated by the irradiation with the excitation light; and recognizing existence of the fluorescent microparticle, starting from the number of signal pulses measured per predetermined standard period, and further includes the step of obtaining the number of signal pulses per standard period with a time interval shorter than the standard period. It further includes the step of counting successively the number of signal pulses generated in the predetermined standard period to recognize existence of the fluorescent microparticle, when the count value exceeds a predetermined threshold, and the kind of the fluorescent microparticles is estimated from the count value. Particularly by using microparticles having a diameter smaller than 0.1 .mu.m as label material, reaction efficiency of the label material is increased, stability of the binding with the material to be measured is raised, and the material to be measured can be detected with a high precision and a high sensitivity.

    摘要翻译： 根据本发明的微粒测量方法，其中计数荧光微粒的数量并分析荧光微粒，包括几乎一个接一个地将荧光微粒引入窄流路的步骤; 用激发光照射窄流路中的荧光微粒; 检测通过用激发光照射产生的荧光的单个光子的检测而产生的信号脉冲; 并且从每个预定标准周期测量的信号脉冲数开始识别荧光微粒的存在，并且还包括以比标准周期短的时间间隔获得每标准周期的信号脉冲数的步骤。 还包括当计数值超过预定阈值时，连续计数在预定标准周期中产生的信号脉冲数，以识别荧光微粒的存在的步骤，并根据计数值估计荧光微粒的种类。 特别是通过使用直径小于0.1μm的微粒作为标签材料，标签材料的反应效率提高，与被测定材料的结合的稳定性提高，可以高检出被测量的材料 精度高，灵敏度高。

    Apparatus for two-dimensional electrophoresis
    52.
    发明授权
    Apparatus for two-dimensional electrophoresis 失效
    二维电泳仪

    公开(公告)号：US4666581A

    公开(公告)日：1987-05-19

    申请号：US728234

    申请日：1985-04-29

    申请人： Michio Itoh ,  Isao Ishikawa ,  Motoko Yoshida ,  Kazunori Okano

    发明人： Michio Itoh ,  Isao Ishikawa ,  Motoko Yoshida ,  Kazunori Okano

    IPC分类号： G01N27/447 ,  G01N27/26

    CPC分类号： G01N27/44773

    摘要： This invention relates to an apparatus for two-dimensional electrophoresis which comprises a supporting plate fixed to a rotary axis, a support for first dimension electrophoresis disposed on the supporting plate and a support for second dimension electrophoresis disposed on another supporting plate. Both the supports are arranged in such a fashion that when the rotary axis is rotated, the support for the first dimension electrophoresis comes on the support for the second dimension electrophoresis or reaches a predetermined position in the support for the second dimension electrophoresis. This arrangement can shift the support for the first dimension electrophoresis to the support for the second dimension electrophoresis without damaging the former.

    摘要翻译： 本发明涉及一种用于二维电泳的装置，其包括固定在旋转轴上的支撑板，设置在支撑板上的第一尺寸电泳用支撑体和设置在另一支撑板上的第二尺寸电泳用支撑体。 两个支撑体都是这样设置的：当旋转轴旋转时，用于第一维电泳的支撑体就在第二维电泳的支撑上，或到达用于第二维电泳的载体中的预定位置。 这种布置可以将第一维电泳的载体转移到用于第二维电泳的载体上，而不损害前者。

    On-line chemical reaction system
    55.
    发明申请
    On-line chemical reaction system 审中-公开
    在线化学反应体系

    公开(公告)号：US20050250145A1

    公开(公告)日：2005-11-10

    申请号：US11122045

    申请日：2005-05-05

    申请人： Atsumu Hirabayashi ,  Yoshinobu Kohara ,  Kazunori Okano

    发明人： Atsumu Hirabayashi ,  Yoshinobu Kohara ,  Kazunori Okano

    IPC分类号： G01N27/62 ,  B01J19/00 ,  C12M1/40 ,  C12Q1/68 ,  G01N1/34 ,  G01N1/40 ,  G01N30/06 ,  G01N30/72 ,  G01N35/10

    CPC分类号： G01N35/1097 ,  G01N1/40 ,  G01N1/405

    摘要： In enzymatic reaction carried out batch-wise, loss of the sample cannot be ignored, and according to the conventional technologies aiming at diminishment of the loss of the sample, a long time is required for reactions. In the present invention, the reaction part in which a chemical substance is immobilized is filled with a sample solution, and the sample solution is held between air at both ends for inhibition of mixing with a buffer solution. The sample solution is provided utilizing a sample introduction part, etc.

    摘要翻译： 在分批进行的酶反应中，不能忽视样品的损失，并且根据旨在减少样品损失的常规技术，反应需要很长时间。 在本发明中，固定有化学物质的反应部分填充有样品溶液，并将样品溶液保持在两端的空气中，以抑制与缓冲溶液的混合。 使用样品引入部件等提供样品溶液

    DNA probe array
    58.
    发明授权
    DNA probe array 有权
    DNA探针阵列

    公开(公告)号：US06288220B1

    公开(公告)日：2001-09-11

    申请号：US09260543

    申请日：1999-03-02

    申请人： Hideki Kambara ,  Kazunori Okano

    发明人： Hideki Kambara ,  Kazunori Okano

    IPC分类号： C07H2104

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00657 ,  B01J2219/00702 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  Y10S436/805 ,  Y10S436/809

    摘要： There is used at least one probe array obtained by arraying particles having various probes, respectively, fixed thereon (probe particles) in a definite order in a holder. A plurality of capillaries or grooves packed with various kinds, respectively, of probe particles are arrayed in parallel, and one of particles contained in each capillary or groove is injected into another capillary or groove to produce a probe array in which the various kinds of probe particles are arrayed in a constant and definite order. Various fluorophore-labeled DNA's are measured at the same time by attaching various probes to particles, respectively, of different sizes. A probe array composed of various fixed DNA probes can easily be produced, and there can be provided a probe array for detecting various DNA's which is composed of various fixed arbitrary DNA probes.

    摘要翻译： 使用至少一个探针阵列，其通过将具有各种探针的颗粒分别在固定器上以确定的顺序分别固定在其上（探针颗粒）而获得。 多个分别装有探针颗粒的毛细管或凹槽平行地排列，并且将每个毛细管或凹槽中包含的一个颗粒注入另一个毛细管或凹槽中以产生探针阵列，其中各种探针 颗粒以恒定且明确的顺序排列。 通过将各种探针分别附着到不同尺寸的颗粒上同时测量各种荧光团标记的DNA。 由各种固定的DNA探针组成的探针阵列可以容易地生成，并且可以提供用于检测由各种固定的任意DNA探针组成的各种DNA的探针阵列。

    Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides
    59.
    发明授权
    Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides 失效
    多核苷酸分析或测定方法，多核苷酸分析仪或仪器

    公开(公告)号：US5861252A

    公开(公告)日：1999-01-19

    申请号：US758220

    申请日：1996-11-27

    申请人： Hideki Kambara ,  Kazunori Okano ,  Chihiro Uematsu

    发明人： Hideki Kambara ,  Kazunori Okano ,  Chihiro Uematsu

    IPC分类号： G01N33/50 ,  C07H21/04 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/68 ,  G01N27/447 ,  G06F17/30 ,  C07K3/14 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q1/683

    摘要： A method of analysis or assay for nucleotides comprises: (1) a step of digesting DNA with a restriction enzyme; (2) a step of discriminating a difference in sequences of the DNA fragments obtained in step (1) above around the 3' termini thereof with a DNA probe and extending the DNA probe by a complementary strand synthesis to fractionate the DNA fragments into groups; and, (3) a step of measuring lengths of the DNA fragments which belong to said groups, or length of the DNA probe extended by said complementary strand extension reaction; wherein the thus measured lengths obtained for every sequence of the bases of the DNA fragments around the 3' termini thereof are employed as fingerprints.

    摘要翻译： 核苷酸的分析或测定方法包括：（1）用限制酶消化DNA的步骤; （2）通过DNA探针区分3'末端的步骤（1）中获得的DNA片段的序列差异的步骤，并通过互补链合成扩增DNA探针，将DNA片段分离成组; 和（3）测量属于所述组的DNA片段的长度或通过所述互补链延伸反应延伸的DNA探针的长度的步骤; 其中将由此测量的长度为其3'末端周围的DNA片段的碱基的每个序列获得的长度用作指纹。

    DNA measuring method
    60.
    发明授权
    DNA measuring method 失效
    DNA测定方法

    公开(公告)号：US5356776A

    公开(公告)日：1994-10-18

    申请号：US942470

    申请日：1992-09-09

    申请人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

    发明人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

    IPC分类号： C12M1/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/70

    CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N27/447 ,  Y10T436/143333

    摘要： DNA molecule length can be measured with high precision and efficiency by 1) using such means as electrophoresis gel migration to orient a DNA molecule having a fluorescence label at both its termini into a straight line by its passing through a migration path having in a portion of it an area not more than several micrometers in diameter, detecting the fluorescence label at both the termini at a predetermined location and measuring the interval between the detection of the fluorescence coming from one terminus and that of the fluorescence from the other or by 2) a DNA molecule bound to a fluorescence label at one terminus and to a particle at the other being led as a whole by such means as electric field application into an aperture smaller in diameter than the particle, leaving the particle fixed at the mouth of the aperture to stretch the DNA molecule and detecting the fluorescence position to measure the distance between the bound particle and the bound fluorescence label.

    摘要翻译： 可以通过以下方式测量DNA分子长度：1）使用电泳凝胶迁移的方式，通过其经过一部分迁移路径将具有两端的荧光标记的DNA分子定向成直线 它是直径不超过几微米的区域，在预定位置的两个末端检测荧光标记，并测量从一个末端检测荧光和来自另一个末端的荧光检测之间的间隔，或者通过2）a 在一个末端与一个荧光标记结合的DNA分子和另一个的一个粒子整体通过电场施加到比颗粒直径更小的孔隙中，将颗粒固定在孔的口处 拉伸DNA分子并检测荧光位置以测量结合的颗粒和结合的荧光标记之间的距离。