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88 条记录 (耗时 0.04 秒)

    Reducing non-specific background in immunofluorescence techniques
    52.
    发明授权
    Reducing non-specific background in immunofluorescence techniques 失效
    减少免疫荧光技术中的非特异性背景

    公开(公告)号：US4582791A

    公开(公告)日：1986-04-15

    申请号：US539872

    申请日：1983-10-07

    申请人： Pyare L. Khanna ,  Jimmy D. Allen ,  Ian Gibbons

    发明人： Pyare L. Khanna ,  Jimmy D. Allen ,  Ian Gibbons

    IPC分类号： G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/53 ,  G01N33/554 ,  G01N33/571

    CPC分类号： G01N33/542 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/825

    摘要： Novel compositions and methods are provided for detecting the presence of a material of interest in a specimen on a solid surface. A composition useful for detecting the presence of a material of interest in a specimen comprises (1) a detector conjugate comprising a fluorescing moiety bonded to a compound capable of specific binding with the material of interest, or a derivative thereof, and (2) a non-detector conjugate comprising a poly(amino acid) and a compound having substantial structural and charge similarity to the fluorescing compound and no observable fluorescence, or low level fluorescence of a different wavelength than that of the fluoresing compound, in the region of fluorescence of the fluorescing compound. In the method of the invention, a specimen, on a solid surface, is combined with the detector conjugate and the non-detector conjugate, and the combination is incubated. Unbound conjugates are removed from the solid surface by washing and then the surface is irradiated with light having a wave length absorbed by the fluorescing compound. The fluorescent light emitted by the combination is observed and is a function of the amount of the material of interest present in the specimen.

    摘要翻译： 提供新的组合物和方法用于检测在固体表面上的样品中感兴趣的材料的存在。 用于检测样品中感兴趣的材料的组合物的组合物包括（1）包含与能够与感兴趣的材料特异性结合的化合物或其衍生物结合的荧光部分的检测器缀合物，和（2） 非检测器缀合物包含聚（氨基酸）和与荧光化合物具有实质的结构和电荷相似性的化合物，并且在荧光区域中不存在与荧光化合物不同的波长的可观察到的荧光或低水平荧光 荧光化合物。 在本发明的方法中，将固体表面上的样品与检测器缀合物和非检测器缀合物组合，并将组合孵育。 通过洗涤从固体表面除去未结合的共轭物，然后用具有由荧光化合物吸收的波长的光照射该表面。 观察到由组合发射的荧光，并且是样品中存在的感兴趣材料的量的函数。

    Immunoassays employing protected labels
    53.
    发明授权
    Immunoassays employing protected labels 失效
    免疫测定采用保护标签

    公开(公告)号：US4578350A

    公开(公告)日：1986-03-25

    申请号：US535452

    申请日：1983-09-23

    申请人： Richard D. Armenta ,  Ian Gibbons

    发明人： Richard D. Armenta ,  Ian Gibbons

    IPC分类号： G01N33/535 ,  G01N33/68 ,  C12N9/96

    CPC分类号： G01N33/68 ,  G01N33/535 ,  Y10S435/962 ,  Y10S435/964 ,  Y10S436/801

    摘要： An improved immunoassay for determining the presence of an analyte in a serum sample and conjugates therefor are described. The known method comprises combining the sample with a conjugate of the analyte and an enzyme and with a receptor for analyte, and determining the presence of analyte in the sample from the effect that the sample has on the enzymatic activity when compared to the enzymatic activity in the absence of analyte or in the presence of known amounts of analyte. The present invention resides in the conjugation to the enzyme label of a label protectant which allows modulation of the enzymatic activity to occur and minimizes background interference between the enzyme label and the sample, which would alter the enzymatic activity.

    摘要翻译： 描述了用于确定血清样品中的分析物的存在和其缀合物的改进的免疫测定。 已知的方法包括将样品与分析物和酶的共轭物与分析物的受体组合，并且与样品中的酶活性相比，测定样品中分析物的存在与酶活性的相关性 不存在分析物或存在已知量的分析物。 本发明在于与标记保护剂的酶标记物的缀合，其允许调节酶活性发生并使酶标签和样品之间的背景干扰最小化，这将改变酶活性。

    MOISTURE SENSOR
    59.
    发明申请
    MOISTURE SENSOR 审中-公开
    水分传感器

    公开(公告)号：US20090035865A1

    公开(公告)日：2009-02-05

    申请号：US11832130

    申请日：2007-08-01

    申请人： Colette Pamela DeMoor ,  Ashok Sinha ,  Ian Gibbons

    发明人： Colette Pamela DeMoor ,  Ashok Sinha ,  Ian Gibbons

    IPC分类号： G01N33/18 ,  B01J19/00 ,  G01N21/78

    CPC分类号： G01N31/222 ,  G01N21/81

    摘要： A moisture sensor is disclosed which measures cumulative exposure to moisture. The moisture sensor comprises a matrix having a hygroscopic material, a first reagent, and a second reagent which interact only in the presence of water to produce a detectable, irreversible change in the matrix to provide a moisture indication. The moisture sensor may be incorporated with a disposable diagnostic device, whereby a method for preventing the use of such a disposable diagnostic device if exposed to excessive cumulative humidity is also disclosed.

    摘要翻译： 公开了一种测量累积暴露于湿气的湿度传感器。 水分传感器包括具有吸湿材料，第一试剂和第二试剂的基质，其仅在水存在下相互作用以在基质中产生可检测的不可逆变化以提供湿气指示。 湿度传感器可以与一次性诊断装置并入，由此也公开了如果暴露于过度累积湿度时防止使用这种一次性诊断装置的方法。