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371 条记录 (耗时 0.039 秒)

    Separation media, multiple electrospray nozzle system and method
    83.
    发明授权
    Separation media, multiple electrospray nozzle system and method 有权
    分离介质，多重电喷雾喷嘴系统及方法

    公开(公告)号：US06956207B2

    公开(公告)日：2005-10-18

    申请号：US10405689

    申请日：2003-04-01

    申请人： Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser ,  Xian Huang

    发明人： Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser ,  Xian Huang

    IPC分类号： G01N27/62 ,  B01D15/08 ,  B01D57/02 ,  B01D59/44 ,  B01J20/281 ,  B01J20/283 ,  B01J20/285 ,  B03C5/00 ,  B05B5/043 ,  G01N1/10 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/447 ,  G01N30/28 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  G01N37/00 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10

    CPC分类号： G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

    摘要： A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.

    摘要翻译： 公开了一种具有分离材料的微细硅芯片，例如在其微通道中原位制备的多孔聚合物整料。 聚合物整料是液体可渗透的，用作液相色谱的微柱，其通过在微通道中含有乙烯基单体和溶剂（致孔剂）的混合物的原位自由基聚合制备。 公开了一种方法和系统，以从一个或多个喷嘴产生一个或多个电喷雾羽流，其提供与质量分析仪测量的离子强度，其大小与为流体中包含的分析形成的电喷雾羽流的数量成比例。 多个电喷雾装置可以以小型化的独立电喷雾装置阵列的形式使用，用于从用于分析的相同流体的多个喷嘴产生多个电喷雾羽流。 与先前公开的系统和方法相比，本发明显着提高了微芯片电喷雾装置的灵敏度。 具有本文公开的包装微通道的硅芯片可以与质谱联用进行样品分析。 还公开了一种分离块，其具有填充有诸如聚合物整料的分离材料的多个通过基底通道，其可以堆叠在多个块中用于顺序的二维色谱分离并与电喷雾装置集成。

    Wallless monolith columns for chromatography
    84.
    发明申请
    Wallless monolith columns for chromatography 审中-公开
    无层析柱

    公开(公告)号：US20050155933A1

    公开(公告)日：2005-07-21

    申请号：US11031495

    申请日：2005-01-07

    申请人： Qi-Feng Ma

    发明人： Qi-Feng Ma

    IPC分类号： B01D15/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/283 ,  C02F1/28 ,  G01N30/52 ,  G01N30/60

    CPC分类号： B01J20/28042 ,  B01D15/22 ,  B01J20/282 ,  B01J20/283 ,  B01J2220/58 ,  B01J2220/82 ,  G01N30/6069 ,  G01N2030/528

    摘要： A monolith column for chromatography comprising a monolith rod encased with a tubular structure wherein the mass of the interior surface layer of said tubular structure intercalate by an encasing process into the surface layer of said monolith rod through the micro-cavities of said surface layer of said monolith rod, forming a hybrid layer shared by both said monolith rod and said tubular structure.

    摘要翻译： 一种用于色谱的整体柱，其包括用管状结构封装的整料，其中所述管状结构的内表面层的质量通过包封工艺插入所述整料棒的表面层中，通过所述表面层的所述表面层的微腔 整料杆，形成由所述整料杆和所述管状结构共同的混合层。

    Protection of the chromatographic bed in dynamic axial compression chromatography devices
    85.
    发明申请
    Protection of the chromatographic bed in dynamic axial compression chromatography devices 失效
    动态轴向压缩色谱装置中色谱床的保护

    公开(公告)号：US20050087494A1

    公开(公告)日：2005-04-28

    申请号：US11010258

    申请日：2004-12-14

    申请人： Wilhelm Hauck ,  Harlene Marks ,  Jean Blehaut ,  Roger Nicoud

    发明人： Wilhelm Hauck ,  Harlene Marks ,  Jean Blehaut ,  Roger Nicoud

    IPC分类号： G01N27/22 ,  B01D15/08 ,  B01J20/291 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/80 ,  G01N30/88

    CPC分类号： B01D15/08 ,  G01N30/52 ,  G01N30/6004 ,  G01N30/6021 ,  G01N2030/522 ,  G01N30/62

    摘要： The invention relates to a chromatography device comprising: a column (12) intended to house, between its two ends (16a, 16b) a chromatographic bed (14) and a fluid to be chromatographed, a fluid layer (48) being defined between a surface of the bed (14) and one (16a) of the ends of the column (12); a piston (36) sliding in the column (12), the piston (36) being one of the ends (16a, 16b) of the column (12); at least a first probe (45, 46) for detecting the surface of the bed (14), the probe (45, 46) being mounted on the end (16a) of the column (12) defining the layer (48); and means (52) for adjusting the position of the piston (36) according to the detection of the surface of the bed (14) by the probe (45, 46). The invention also relates to processes for separating at least two compounds of a fluid to be chromatographed and to methods of adjusting the position of the piston with respect to the surface of the chromatographic bed using the chromatography device. The invention has the advantage of allowing effective separation of the compounds of the fluid to be chromatographed while ensuring that the bed is protected.

    摘要翻译： 本发明涉及一种色谱装置，其包括：用于容纳其两端（16a，16b）色谱床（14）和要进行色谱分离的流体之间的柱（12），被定义为流体层（48） 在床（14）的表面和柱（12）的端部的一个（16a）之间; 活塞（36），其在所述塔（12）中滑动，所述活塞（36）是所述塔（12）的端部（16a，16b）之一; 至少一个用于检测床（14）表面的第一探针（45,46），探针（45,46）安装在限定层（48）的柱（12）的端部（16a）上; 以及用于根据由探针（45,46）检测床（14）的表面来调节活塞（36）的位置的装置（52）。 本发明还涉及用于分离至少两种待层析的流体的化合物的方法以及使用色谱装置相对于色谱床的表面调节活塞的位置的方法。 本发明具有允许有效分离要进行色谱分离的流体的化合物同时确保床被保护的优点。

    Cast membrane structures for sample preparation
    87.
    发明授权
    Cast membrane structures for sample preparation 有权
    用于样品制备的铸膜结构

    公开(公告)号：US06875354B1

    公开(公告)日：2005-04-05

    申请号：US09387443

    申请日：1999-09-01

    申请人： William Kopaciewicz

    发明人： William Kopaciewicz

    IPC分类号： G01N1/10 ,  B01D61/00 ,  B01D61/18 ,  B01D63/00 ,  B01D63/16 ,  B01D67/00 ,  B01D69/10 ,  B01D69/14 ,  B01J20/28 ,  B01L3/02 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  G01N1/36 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N35/10 ,  G01N15/06

    CPC分类号： B01J20/28033 ,  B01D61/00 ,  B01D61/007 ,  B01D61/18 ,  B01D63/16 ,  B01D69/10 ,  B01D69/141 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/28026 ,  B01J20/28028 ,  B01J20/2803 ,  B01J2220/64 ,  B01L3/0275 ,  G01N30/466 ,  G01N30/6004 ,  G01N30/6052 ,  G01N30/6065 ,  G01N2030/528 ,  G01N2035/1053 ,  G01N2035/1055

    摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.

    摘要翻译： 用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。 可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。 在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。 通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。 本发明还包括复合结构以及含有该结构的样品制备装置。 在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。 本发明还包括这些结构以及包含这些结构的壳体。

    Hydrophilic monolithic columns for separation of saccharide mixtures
    90.
    发明授权
    Hydrophilic monolithic columns for separation of saccharide mixtures 失效
    用于分离糖混合物的亲水性整体柱

    公开(公告)号：US06858135B2

    公开(公告)日：2005-02-22

    申请号：US10634058

    申请日：2003-08-04

    申请人： Amy H. Que ,  Milos V. Novotny

    发明人： Amy H. Que ,  Milos V. Novotny

    IPC分类号： B01J20/285 ,  B01J20/291 ,  G01N30/28 ,  G01N30/52 ,  B01D15/08

    CPC分类号： B01J20/261 ,  B01J20/265 ,  B01J20/285 ,  B01J20/291 ,  B01J2220/54 ,  B01J2220/82 ,  G01N2030/285 ,  G01N2030/528

    摘要： Polar, monolithic CEC columns having an amine and/or cyano functional stationary phase are used to separate complex mixtures of saccharides. Using the positive-ion mode of detection for neutral saccharides, detection conditions were optimized to low-femtomole sensitivities with the use of an ion trap mass spectrometer. The described column technology provides a universal system for separating a wide range of carbohydrates: mono- and oligosaccharides with the intact reducing end, as well as saccharide alditols.

    摘要翻译： 使用具有胺和/或氰基官能化固定相的极性单片CEC柱来分离糖的复杂混合物。 使用中性糖的正离子检测方式，使用离子阱质谱仪将检测条件优化为低飞镖的敏感性。 所描述的柱技术提供了用于分离广泛范围的碳水化合物的通用体系：具有完整还原末端的单糖和寡糖以及糖类糖醇。