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公开(公告)号:US09126160B2
公开(公告)日:2015-09-08
申请号:US12963523
申请日:2010-12-08
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: A system, including method and apparatus, for forming an array of emulsions. The system may include a plate providing an array of emulsion production units each configured to produce a separate emulsion and each including a set of wells interconnected by channels that intersect to form a site of droplet generation. Each set of wells, in turn, may include (1) at least one first input well to receive a continuous phase, (2) a second input well to receive a dispersed phase, and (3) an output well configured to receive from the site of droplet generation an emulsion of droplets of the dispersed phase disposed in the continuous phase.
摘要翻译: 一种用于形成乳剂阵列的系统,包括方法和装置。 该系统可以包括提供乳液生产单元阵列的板,每个乳液生产单元被配置为产生单独的乳液,并且每个包括通过相交以形成液滴产生位点的通道相互连接的一组孔。 每组井又可包括(1)至少一个用于接收连续相的第一输入井,(2)第二输入井以接收分散相,以及(3)输出孔,其构造成从 产生液滴的位置是分散相的液滴的乳液,其设置在连续相中。
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公开(公告)号:US09194861B2
公开(公告)日:2015-11-24
申请号:US12862542
申请日:2010-08-24
CPC分类号: B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
摘要: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
摘要翻译: 系统,包括方法,装置,组合物和试剂盒,用于通过多个乳液的聚结来混合小体积的流体。
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公开(公告)号:US20110212516A1
公开(公告)日:2011-09-01
申请号:US12890550
申请日:2010-09-24
IPC分类号: C12M1/00
CPC分类号: B01F3/0807 , B01F13/0062 , B01L3/0241 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/0622
摘要: Thermocycling system, including methods and apparatus, for performing a flow-based reaction on a sample in fluid. The system may include a plurality of segments defining at least two temperature regions, and also may include a plurality of heating elements configured to maintain each temperature region at a different desired temperature. At least one of the heating elements may be a thermoelectric cooler operatively disposed to transfer heat to and/or from a temperature region The system further may include a fluid channel extending along a helical path that passes through the temperature regions multiple times such that fluid flowing in the channel is heated and cooled cyclically.
摘要翻译: 热循环系统,包括用于对流体中的样品进行基于流的反应的方法和装置。 该系统可以包括限定至少两个温度区域的多个段,并且还可以包括被配置为将每个温度区域保持在不同的期望温度的多个加热元件。 加热元件中的至少一个可以是可操作地设置成将热传递到和/或从温度区域传递的热电冷却器。该系统还可以包括沿着螺旋路径延伸的流体通道,该螺旋路径多次通过温度区域,使得流体流动 在通道中循环加热和冷却。
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公开(公告)号:US20110053798A1
公开(公告)日:2011-03-03
申请号:US12862542
申请日:2010-08-24
CPC分类号: B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
摘要: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
摘要翻译: 系统,包括方法,装置,组合物和试剂盒,用于通过多个乳液的聚结来混合小体积的流体。
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公开(公告)号:US08633015B2
公开(公告)日:2014-01-21
申请号:US12890550
申请日:2010-09-24
IPC分类号: C12M1/34
CPC分类号: B01F3/0807 , B01F13/0062 , B01L3/0241 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/0622
摘要: Thermocycling system, including methods and apparatus, for performing a flow-based reaction on a sample in fluid. The system may include a plurality of segments defining at least two temperature regions, and also may include a plurality of heating elements configured to maintain each temperature region at a different desired temperature. At least one of the heating elements may be a thermoelectric cooler operatively disposed to transfer heat to and/or from a temperature region The system further may include a fluid channel extending along a helical path that passes through the temperature regions multiple times such that fluid flowing in the channel is heated and cooled cyclically.
摘要翻译: 热循环系统,包括用于对流体中的样品进行基于流的反应的方法和装置。 该系统可以包括限定至少两个温度区域的多个段,并且还可以包括被配置为将每个温度区域保持在不同的期望温度的多个加热元件。 加热元件中的至少一个可以是可操作地设置成将热传递到和/或从温度区域传递的热电冷却器。该系统还可以包括沿着螺旋路径延伸的流体通道,该螺旋路径多次通过温度区域,使得流体流动 在通道中循环加热和冷却。
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公开(公告)号:US20130084572A1
公开(公告)日:2013-04-04
申请号:US13251016
申请日:2011-09-30
CPC分类号: G01N21/6428 , C12Q1/6851 , G01N2021/6441 , C12Q2545/101 , C12Q2563/159
摘要: System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.
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公开(公告)号:US20120152369A1
公开(公告)日:2012-06-21
申请号:US13287120
申请日:2011-11-01
申请人: Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Steenblock
发明人: Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Steenblock
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F13/1022 , B01L3/502761 , B01L2200/0605 , B01L2200/0673 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2035/1046 , Y10T137/0396 , Y10T137/8593
摘要: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.
摘要翻译: 用于形成乳液的系统,包括方法,装置和试剂盒。 该系统可以包括由仪器接收的仪器和微流体芯片。 仪器可以对由芯片保持的预期乳液相施加压力,以驱动芯片中的乳液的形成和收集。 在一些实施例中,当仪器检测到满足预定条件的压力变化时,仪器可能停止向芯片施加压力。 该变化可能表明已达到液滴产生的终点。
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公开(公告)号:US09089844B2
公开(公告)日:2015-07-28
申请号:US13287120
申请日:2011-11-01
申请人: Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia
发明人: Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F13/1022 , B01L3/502761 , B01L2200/0605 , B01L2200/0673 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2035/1046 , Y10T137/0396 , Y10T137/8593
摘要: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.
摘要翻译: 用于形成乳液的系统,包括方法,装置和试剂盒。 该系统可以包括由仪器接收的仪器和微流体芯片。 仪器可以对由芯片保持的预期乳液相施加压力,以驱动芯片中的乳液的形成和收集。 在一些实施例中,当仪器检测到满足预定条件的压力变化时,仪器可能停止向芯片施加压力。 该变化可能表明已达到液滴产生的终点。
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公开(公告)号:US09500664B2
公开(公告)日:2016-11-22
申请号:US13341669
申请日:2011-12-30
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01L3/0275 , B01L3/502715 , B01L3/502723 , B01L7/52 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2400/0487 , C12Q1/6806 , G01N35/085 , G01N35/1065 , G01N2035/00148 , G01N2035/1034 , Y10T29/494
摘要: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
摘要翻译: 一种用于产生适合于基于液滴的测定的液滴的方法和装置的系统。 所公开的系统可以包括通过将含水样品的流体与背景乳液流体(例如油)并入而形成含有样品的液滴的乳液,其被配置成通过将含水样品的流体与背景乳液流体例如油混合而构成用于形成含样品的液滴的单件或多件式液滴产生组件 在背景液中。 在一些情况下,所公开的系统可以包括被配置成将含样品的液滴输送到出口区域的通道或其它合适的机制,从而可以执行随后的测定步骤。
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公开(公告)号:US08730479B2
公开(公告)日:2014-05-20
申请号:US13341678
申请日:2011-12-30
IPC分类号: G01N21/00
CPC分类号: G01N21/6428 , B01L3/502784 , G01N15/1456 , G01N21/05 , G01N21/31 , G01N21/553 , G01N21/645 , G01N21/6486 , G01N2021/0346 , G01N2021/052 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/8564 , G01N2201/062 , G01N2201/0627 , G01N2201/0696
摘要: System, including methods and apparatus, for light detection and signal processing for droplet-based assays. An exemplary system provides a method of detection for droplets. An examination region of a channel may be illuminated with first pulses of light interleaved with second pulses of light as droplets pass through the examination region, the first pulses being spectrally distinct from the second pulses. Data may be collected representing light detected during illumination of the examination region with the first pulses and the second pulses. Each droplet may be illuminated with a beam of light that is narrower than a diameter of the droplets.
摘要翻译: 用于基于液滴的测定的光检测和信号处理的系统,包括方法和装置。 示例性系统提供了液滴的检测方法。 当液滴通过检查区域时,可以用与第二脉冲光交错的光的第一脉冲照亮通道的检查区域,第一脉冲在光谱上与第二脉冲不同。 可以收集表示利用第一脉冲和第二脉冲在检查区域的照明期间检测到的光的数据。 可以用比液滴直径窄的光束照射每个液滴。
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