公开(公告)号：US08934098B2

公开(公告)日：2015-01-13

申请号：US12873132

申请日：2010-08-31

Applicant: David Cox ,  Marc Haberstroh ,  Patrick Kinney ,  Albert Carrillo ,  Jon Hoshizaki ,  Maryam Shariati ,  Howard King ,  Joe Lee ,  Matthew Chan

Inventor： David Cox ,  Marc Haberstroh ,  Patrick Kinney ,  Albert Carrillo ,  Jon Hoshizaki ,  Maryam Shariati ,  Howard King ,  Joe Lee ,  Matthew Chan

IPC: G01N21/25 ,  C12Q1/68 ,  B01F13/00 ,  B01F13/02 ,  B01J19/00 ,  B01L3/00 ,  G01N35/00 ,  G01N35/10 ,  G02B21/34

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器） 轮可以被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。该系统还可以被配置为考虑快速启动和停止快速索引所产生的振动 滤光轮以及由其他光源产生的振动。本文还提供了在多个波长下快速成像样品的各种方法。

公开(公告)号：US20150004717A1

公开(公告)日：2015-01-01

申请号：US14319497

申请日：2014-06-30

Applicant: William Marsh Rice University

Inventor： John MCDEVITT ,  Ahmed HAQUE ,  Michael MCRAE ,  Glen SIMMONS

IPC: G01N35/00

CPC classification number: G01N35/00029 ,  G01N35/1002 ,  G01N2035/00039 ,  G01N2035/00148

Abstract: This disclosure describes the design and function of a bead-based lab-on-a-chip portable analyzer for analyzing biomarker assay cards used in point-of-care testing.

Abstract translation: 本公开描述了用于分析用于点护理测试中的生物标志物测定卡的基于珠的实验室上的便携式分析仪的设计和功能。

公开(公告)号：US08889596B2

公开(公告)日：2014-11-18

申请号：US13969195

申请日：2013-08-16

Applicant: Life Technologies Corporation

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/68 ,  G01N35/10 ,  B01J19/00 ,  B01L3/00 ,  B01F13/00 ,  B01F13/02 ,  G01N35/00 ,  G02B21/34

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

Abstract translation: 根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。 洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。 洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。 在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。 在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。

公开(公告)号：US08841118B2

公开(公告)日：2014-09-23

申请号：US12800467

申请日：2010-05-14

Applicant: Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

Inventor： Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

IPC: C12M1/00 ,  C12M3/00 ,  G01N35/00 ,  C12M1/34 ,  C12Q1/04 ,  G01N35/04

CPC classification number: C12Q1/04 ,  C12M41/36 ,  C12Q1/02 ,  G01N35/00603 ,  G01N35/0099 ,  G01N35/026 ,  G01N2035/00148 ,  G01N2035/0406 ,  G01N2035/0453 ,  G01N2035/0465

Abstract: A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.

Abstract translation: 检测仪器确定样品容器（例如血培养瓶）是否对其中存在微生物剂生长是阳性的。 当容器被认为是正的时，它被提供（例如，转移或暴露于）到执行微生物剂的鉴定和/或表征的自动化仪器。 识别和/或表征仪器将样品的一部分从样品容器中取出并将其放入一次性分离和浓缩装置中。 微生物剂通过选择性裂解可能存在的和非离子的非微生物剂细胞材料而浓缩。 阅读模块使用光谱方法读取浓缩的微生物剂，例如固有荧光的测量。 当微生物剂保持集中在一次性装置中时，可能发生这种询问。

公开(公告)号：US20140170645A1

公开(公告)日：2014-06-19

申请号：US13967957

申请日：2013-08-15

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后净化模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适合不超过10英尺3的外壳。 并且可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。

公开(公告)号：US20140154731A1

公开(公告)日：2014-06-05

申请号：US14173157

申请日：2014-02-05

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Kurt J. Halverson

IPC: C12Q1/02

CPC classification number: C12Q1/02 ,  G01N1/4077 ,  G01N35/00029 ,  G01N2001/4088 ,  G01N2035/00148 ,  Y10T436/255 ,  Y10T436/2575

Abstract: A system and method for processing samples. The system can include a loading chamber, a detection chamber positioned in fluid communication with the loading chamber, and a fluid path defined at least partially by the loading chamber and the detection chamber. The system can further include a filter positioned such that at least one of its inlet and its outlet is positioned in the fluid path. The method can include positioning a sample in the loading chamber, filtering the sample in the fluid path to form a concentrated sample and a filtrate, removing the filtrate from the fluid path at a location upstream of the detection chamber, moving at least a portion of the concentrated sample in the fluid path to the detection chamber, and analyzing at least a portion of the concentrated sample in the detection chamber for an analyte of interest.

Abstract translation: 一种用于处理样品的系统和方法。 该系统可以包括装载室，与装载室流体连通的检测室，以及至少部分地由装载室和检测室限定的流体路径。 该系统还可以包括过滤器，其被定位成使得其入口和出口中的至少一个定位在流体路径中。 该方法可以包括将样品定位在装载室中，在流体路径中过滤样品以形成浓缩的样品和滤液，在检测室上游的位置从流体路径移除滤液，移动至少一部分 所述浓缩样品在所述检测室的流体路径中，以及分析所述检测室中所述浓缩样品的至少一部分，用于感兴趣的分析物。

公开(公告)号：US08697007B2

公开(公告)日：2014-04-15

申请号：US12535718

申请日：2009-08-05

Applicant: Haim H. Bau ,  Changchun Liu ,  Xianbo Qiu

Inventor： Haim H. Bau ,  Changchun Liu ,  Xianbo Qiu

IPC: B01L3/00 ,  G01N33/00 ,  G01N33/50

CPC classification number: G01N35/0092 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/0819 ,  B01L2300/0825 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N35/0099 ,  G01N2035/00148

Abstract: Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.

Abstract translation: 提供了分析盒，其包括可移除的密封层和可变形的连接器层，其将各个通道，室和储存器放置在盒体上彼此流体连通。 盒子适用于可以自动化操作的自包含免疫测定装置。 还公开了通过使用所公开的盒分析样品的方法。

公开(公告)号：US08647836B2

公开(公告)日：2014-02-11

申请号：US13684910

申请日：2012-11-26

Applicant: Microm International GmbH

Inventor： Hans L. Heid ,  Jose Novoa

IPC: G01N1/28 ,  C12M3/00 ,  B01L3/00 ,  G01N35/00 ,  B32B37/00

CPC classification number: C12Q1/04 ,  G01N1/06 ,  G01N1/312 ,  G01N35/00732 ,  G01N2035/00148 ,  G01N2035/00782 ,  G06F19/00

Abstract: The invention relates to a method and device for the cross-referencing of identification (1) of tissue slice supports (2), for microtomised analytical samples still to be mounted thereon, with identification information (3) of a tissue sample holder (4) of a tissue sample (5) which is not yet microtomised. The conventional problem of cross-referencing is improved in a simple manner, whereby the identification information (3) for the tissue sample holder (4) is automatically detected when positioned in the microtome (6) and an identification (1) corresponding thereto is automatically transferred to at least one tissue slice support (2) and that tissue slice support (2), provided with the identification (1), is dispensed for application of the tissue sample slice at the moment when a tissue sample slice must be applied to a tissue slice support (2).

Abstract translation: 本发明涉及一种组织切片支架（2）的识别信息（3）与组织样本支架（4）的识别信息（3）进行交叉参考的方法和装置，用于还有待安装在其上的微量分析样品， 的尚未显微切割的组织样品（5）。 以简单的方式改进了交叉参照的常规问题，由此当定位在切片机（6）中时自动检测组织样本保持器（4）的识别信息（3），并且与其对应的识别符（1）自动地 转移到至少一个组织切片支撑件（2），并且在组织样本切片必须施加于一个组织切片支架（2）的那一刻被分配用于施用组织样本切片的设置有标识（1）的组织切片支撑件（2） 组织切片支架（2）。

公开(公告)号：US08574495B2

公开(公告)日：2013-11-05

申请号：US12469355

申请日：2009-05-20

Applicant: Marc Pellicer Sancho

Inventor： Marc Pellicer Sancho

IPC: G01N21/00

CPC classification number: G01N35/025 ,  G01N35/026 ,  G01N2035/00148 ,  G01N2035/0425 ,  Y10T436/2575

Abstract: The apparatus comprises a single transportable structure provided with several separate compartments located in a vertical stack within a cabinet, with means for the vertical transport of gel cards, means for the transport of units for moving the cards and pipetting probes, being displaceable to the entire transverse cross-section of the compartments in at least two of the vertical compartments or floors through moving along coordinate axes X, Y, Z at right-angles, and control means so that automatic functioning can take place simultaneously with manual actions to load and unload the necessary materials (samples, reagents, cards, wash solutions and waste solutions) in a random manner.

Abstract translation: 该装置包括单个可运输结构，其设置有位于机柜内的垂直堆叠中的几个单独的隔间，具有用于垂直运输凝胶卡的装置，用于运输用于移动卡和移液探针的单元的装置，可移位到整个 通过沿着坐标轴X，Y，Z以直角移动，并且控制装置在至少两个垂直隔间或地板中的隔室的横截面，使得自动功能可以与手动操作同时进行加载和卸载 必要的材料（样品，试剂，卡，洗液和废液）。

公开(公告)号：US08394642B2

公开(公告)日：2013-03-12

申请号：US12795515

申请日：2010-06-07

Applicant: Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.