公开(公告)号：US07906072B2

公开(公告)日：2011-03-15

申请号：US12538641

申请日：2009-08-10

Applicant: Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/00 ,  G01N15/06

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：US20110053806A1

公开(公告)日：2011-03-03

申请号：US12867607

申请日：2009-02-20

Applicant: Yusuf D. Amin

Inventor： Yusuf D. Amin

IPC: C40B60/00

CPC classification number: B01J19/0046 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/043 ,  B01L2300/123 ,  B01L2300/1805

Abstract: A carrier for holding a microfluidic device includes a substrate with a plurality of wells, each well defining a volume of between 0.1 μl and 100 μl; a plurality of channels within the substrate wherein each well is in fluid communication with at least one of the plurality of channels; and a receiving portion for receiving a microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells. The carrier has a polymeric composition and/or an array of structural features that enhance its performance and compatibility with existing instrumentation.

Abstract translation: 用于保持微流体装置的载体包括具有多个孔的底物，每个孔限定0.1μl和100μl之间的体积; 衬底内的多个通道，其中每个阱与所述多个通道中的至少一个流体连通; 以及用于接收微流体装置并将微流体装置与多个孔流体连通的接收部分。 载体具有增强其性能和与现有仪器的兼容性的聚合物组合物和/或结构特征阵列。

公开(公告)号：US07883669B2

公开(公告)日：2011-02-08

申请号：US11408612

申请日：2006-04-20

Applicant: Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: B01L3/02

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: Systems for managing workflows to perform chemical or biological reactions using microfluidic devices.

Abstract translation: 用于管理使用微流体装置进行化学或生物反应的工作流的系统。

公开(公告)号：US07867763B2

公开(公告)日：2011-01-11

申请号：US11058106

申请日：2005-02-14

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。

公开(公告)号：US07820427B2

公开(公告)日：2010-10-26

申请号：US11531147

申请日：2006-09-12

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12M1/38

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：US07815868B1

公开(公告)日：2010-10-19

申请号：US11680541

申请日：2007-02-28

Applicant: Robert C. Jones ,  Paul Wyatt ,  Antoine Daridon ,  Jing Wang ,  Andrew May ,  David Cohen

Inventor： Robert C. Jones ,  Paul Wyatt ,  Antoine Daridon ,  Jing Wang ,  Andrew May ,  David Cohen

IPC: B01J19/00

CPC classification number: B01J19/00 ,  B01F5/0085 ,  B01F5/0602 ,  B01F13/0069 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00355 ,  B01J2219/00389 ,  B01J2219/00418 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0605 ,  B01L2400/0655

Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Abstract translation: SBS格式的微流体装置，其中板的几何形状限定了询问区域阵列，并且其中每个询问区域包含至少一个反应位点。

公开(公告)号：US07792345B2

公开(公告)日：2010-09-07

申请号：US12552208

申请日：2009-09-01

Applicant: Colin Jon Taylor ,  Gang Sun ,  Simant Dube

Inventor： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

IPC: G06K9/00

CPC classification number: G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

Abstract: A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

Abstract translation: 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

公开(公告)号：US20100184202A1

公开(公告)日：2010-07-22

申请号：US12579347

申请日：2009-10-14

Applicant: Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

Inventor： Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

IPC: C12M1/34

CPC classification number: C12Q1/68 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/686 ,  G01N27/453

Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US20100183481A1

公开(公告)日：2010-07-22

申请号：US12573623

申请日：2009-10-05

Applicant: Geoffrey Richard Facer ,  Hany Ramaz Nassef

Inventor： Geoffrey Richard Facer ,  Hany Ramaz Nassef

IPC: B01L9/00 ,  B01L3/00

CPC classification number: B01L3/00 ,  B01J19/0093 ,  B01J2219/0081 ,  B01J2219/00891 ,  B01J2219/0095 ,  B01L3/5027 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/105 ,  B01L2400/0487 ,  B01L2400/0605 ,  Y10T117/10 ,  Y10T117/1004 ,  Y10T117/1024 ,  Y10T117/1064 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Carriers or holders for holding microfluidic devices are provided. Some of the carriers that are provided include a hydration control device and/or a source of controlled fluid pressure to facilitate use of the carrier in conducting various types of analyses.

Abstract translation: 提供用于保持微流体装置的载体或保持器。 提供的一些载体包括水合控制装置和/或受控流体压力源，以便于使用载体进行各种类型的分析。

公开(公告)号：US07704735B2

公开(公告)日：2010-04-27

申请号：US11740735

申请日：2007-04-26

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。