公开(公告)号：US08852448B2

公开(公告)日：2014-10-07

申请号：US12921217

申请日：2009-03-12

Applicant: Dong-Seob Kim ,  Kun-Hong Lee ,  Woon-Bong Hwang ,  Geun-Bae Lim ,  Hyun-Chul Park ,  Byeong-Joo Lee ,  Sang-Min Lee ,  Joon-Won Kim

Inventor： Dong-Seob Kim ,  Kun-Hong Lee ,  Woon-Bong Hwang ,  Geun-Bae Lim ,  Hyun-Chul Park ,  Byeong-Joo Lee ,  Sang-Min Lee ,  Joon-Won Kim

IPC: H01L21/302 ,  H01L21/461 ,  H01L21/311 ,  B44C1/22 ,  B23P15/00 ,  C03C25/00 ,  C23F1/00 ,  B29C33/42 ,  C23C2/26 ,  B29C39/34 ,  C23C2/12 ,  C25D11/04

CPC classification number: C23C2/12 ,  B29C33/424 ,  B29C39/34 ,  C23C2/26 ,  C25D11/04

Abstract: A method for fabricating a 3D (three-dimensional) structure is disclosed to provide hydrophobicity to a surface of a 3D structure by using a dipping method in which a predetermined-shaped structure is immersed in a molten metal solution. The method includes: immersing a predetermined-shaped structure in a molten metal solution to coat a molten metal material on the surface of the predetermined-shaped structure; anodizing a metal base coated with the molten metal material; coating a polymer material on an outer surface of the metal-coated base to form a negative replica structure; covering an outer surface of the negative replica structure with an outer formation material; and removing the metal-coated base from the negative replica structure and the outer formation material.

Abstract translation: 公开了一种用于制造3D（三维）结构的方法，通过使用将预定形状的结构浸入熔融金属溶液中的浸渍方法来提供对3D结构的表面的疏水性。 该方法包括：将预定形状的结构浸入熔融金属溶液中以在预定形状的结构的表面上涂覆熔融金属材料; 阳极氧化涂有熔融金属材料的金属基底; 在金属涂覆的基底的外表面上涂覆聚合物材料以形成负的复制结构; 用外部形成材料覆盖负重复结构的外表面; 以及从阴性复制结构和外部形成材料中去除金属涂覆的基底。

公开(公告)号：US08257630B2

公开(公告)日：2012-09-04

申请号：US12921585

申请日：2009-03-12

Applicant: Dong-Seob Kim ,  Kun-Hong Lee ,  Woon-Bong Hwang ,  Geun-Bae Lim ,  Hyun-Chul Park ,  Byeong-Joo Lee ,  Sang-Min Lee ,  Joon-Won Kim

Inventor： Dong-Seob Kim ,  Kun-Hong Lee ,  Woon-Bong Hwang ,  Geun-Bae Lim ,  Hyun-Chul Park ,  Byeong-Joo Lee ,  Sang-Min Lee ,  Joon-Won Kim

IPC: B29C39/12

CPC classification number: B29C39/12 ,  B29C33/424 ,  B29C33/485 ,  B29C39/34 ,  B29L2031/756 ,  C25D11/045 ,  C25D11/16 ,  C25D11/24

Abstract: A method for fabricating a 3D (three-dimensional) structure such that the 3D structure has a surface with hydrophobicity by using a metal foil such as an aluminum foil is disclosed. The method includes preparing a metal foil base by attaching a metal foil on an outer surface of a predetermined-shaped 3D structure; anodizing the metal foil base; coating a polymer material on the outer surface of the metal foil base material to form a negative replica structure; forming an outer structure by covering an outer surface of the negative replica structure with an outer formation material; and removing the metal foil base.

Abstract translation: 公开了一种用于制造3D（三维）结构的方法，使得3D结构具有通过使用诸如铝箔的金属箔具有疏水性的表面。 该方法包括通过在预定形状的3D结构的外表面上附着金属箔来制备金属箔基底; 阳极氧化金属箔基底; 在金属箔基材的外表面上涂覆聚合物材料以形成负的复制结构; 通过用外部形成材料覆盖所述负面复制结构的外表面来形成外部结构; 并移除金属箔基底。

公开(公告)号：US07863140B2

公开(公告)日：2011-01-04

申请号：US11755952

申请日：2007-05-31

Applicant: Geun-Bae Lim ,  Chin-Sung Park ,  Yoon-Kyoung Cho ,  Sun-Hee Kim

Inventor： Geun-Bae Lim ,  Chin-Sung Park ,  Yoon-Kyoung Cho ,  Sun-Hee Kim

IPC: H01L21/336 ,  G01N27/02 ,  C12M1/34

CPC classification number: G01N33/5438 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00743 ,  B01L3/5027 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  G01N27/4145 ,  G01N33/54373 ,  C12Q2565/607

Abstract: A molecular detection chip including a metal oxide silicon-field effect transistor (MOSFET) on sidewalls of a micro-fluid channel and a molecular detection device including the molecular detection chip are provided. A molecular detection method, particularly, qualification methods for the immobilization of molecular probes and the binding of a target sample to the molecular probes, using the molecular detection device, and a nucleic acid mutation assay device and method are also provided. The formation of the MOSFET on the sidewalls of the micro-fluid channel makes easier to highly integrate a molecular detection chip. In addition, immobilization of probes directly on the surface of a gate electrode ensures the molecular detection chip to check for the immobilization of probes and coupling of a target molecule to the probes in situ.

Abstract translation: 提供了包括在微流体通道的侧壁上的金属氧化物硅场效应晶体管（MOSFET）和包括分子检测芯片的分子检测装置的分子检测芯片。 还提供了使用分子检测装置的分子检测方法，特别是用于固定分子探针的鉴定方法和靶样品与分子探针的结合，以及核酸突变测定装置和方法。 在微流体通道的侧壁上形成MOSFET更容易高度集成分子检测芯片。 此外，探针直接固定在栅电极的表面上确保了分子检测芯片，以检查探针的固定和目标分子与探针原位偶联。

公开(公告)号：US20100126873A1

公开(公告)日：2010-05-27

申请号：US12452873

申请日：2008-03-12

Applicant: Dong-Seob Kim ,  Dong-Hyun Kim ,  Woon-Bong Hwang ,  Hyun-Chul Park ,  Kun-Hong Lee ,  Geun-Bae Lim ,  Sang-Min Lee ,  Joon-Won Kim

Inventor： Dong-Seob Kim ,  Dong-Hyun Kim ,  Woon-Bong Hwang ,  Hyun-Chul Park ,  Kun-Hong Lee ,  Geun-Bae Lim ,  Sang-Min Lee ,  Joon-Won Kim

IPC: C25D11/18 ,  C25D11/16

CPC classification number: C23C28/00 ,  C23C28/04 ,  C25D11/045 ,  C25D11/16 ,  C25D11/24

Abstract: The present invention relates to a manufacturing method of a three dimensional structure having a hydrophobic inner surface. The manufacturing method includes anodizing a three dimensional metal member and forming fine holes on an external surface of the metal member, forming a replica by coating a non-wetting polymer material on the outer surface of the metal member and forming the non-wetting polymer material to be a replication structure corresponding to the fine holes of the metal member, forming an exterior by surrounding the replication structure with an exterior forming material, and etching the metal member and eliminating the metal member from the replication structure and the exterior forming material.

Abstract translation: 本发明涉及具有疏水性内表面的三维结构体的制造方法。 该制造方法包括阳极氧化三维金属构件并在金属构件的外表面上形成细孔，通过在金属构件的外表面上涂覆非润湿聚合物材料并形成非湿润聚合物材料形成复制品 作为与金属构件的细孔对应的复制结构，通过用外部形成材料围绕复制结构形成外部，并且蚀刻金属构件并从复制结构和外部形成材料中除去金属构件。

公开(公告)号：US20100015695A1

公开(公告)日：2010-01-21

申请号：US12504213

申请日：2009-07-16

Applicant: Yoon-kyoung CHO ,  Joon-ho KIM ,  Kak NAMKOONG ,  Geun-bae LIM ,  Jun-hong Min

Inventor： Yoon-kyoung CHO ,  Joon-ho KIM ,  Kak NAMKOONG ,  Geun-bae LIM ,  Jun-hong Min

IPC: C12M1/00

CPC classification number: G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

Abstract: A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

公开(公告)号：US20080093229A1

公开(公告)日：2008-04-24

申请号：US11756083

申请日：2007-05-31

Applicant: Geun-Bae Lim ,  Chin-Sung Park ,  Yoon-Kyoung Cho ,  Sun-Hee Kim

Inventor： Geun-Bae Lim ,  Chin-Sung Park ,  Yoon-Kyoung Cho ,  Sun-Hee Kim

IPC: G01N27/26

CPC classification number: G01N33/5438 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00743 ,  B01L3/5027 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  G01N27/4145 ,  G01N33/54373 ,  C12Q2565/607

Abstract: A molecular detection chip including a metal oxide silicon-field effect transistor (MOSFET) on sidewalls of a micro-fluid channel and a molecular detection device including the molecular detection chip are provided. A molecular detection method, particularly, qualification methods for the immobilization of molecular probes and the binding of a target sample to the molecular probes, using the molecular detection device, and a nucleic acid mutation assay device and method are also provided. The formation of the MOSFET on the sidewalls of the micro-fluid channel makes easier to highly integrate a molecular detection chip. In addition, immobilization of probes directly on the surface of a gate electrode ensures the molecular detection chip to check for the immobilization of probes and coupling of a target molecule to the probes in situ.

Abstract translation: 提供了包括在微流体通道的侧壁上的金属氧化物硅场效应晶体管（MOSFET）和包括分子检测芯片的分子检测装置的分子检测芯片。 还提供了使用分子检测装置的分子检测方法，特别是用于固定分子探针的鉴定方法和靶样品与分子探针的结合，以及核酸突变测定装置和方法。 在微流体通道的侧壁上形成MOSFET更容易高度集成分子检测芯片。 此外，探针直接固定在栅电极的表面上确保了分子检测芯片，以检查探针的固定和目标分子与探针原位偶联。

公开(公告)号：US07329535B2

公开(公告)日：2008-02-12

申请号：US10292012

申请日：2002-11-11

Applicant: Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

Inventor： Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

IPC: C12M1/34 ,  C12M3/00 ,  F16K13/00 ,  B01L11/00 ,  B32B5/02

CPC classification number: B01L7/525 ,  B01L3/502738 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  Y10T137/4643 ,  Y10T137/4651

Abstract: Provided are an apparatus for circulating a carrier fluid having two or more chambers or sections, an apparatus for amplifying a nucleic acid using the same, and a chip containing the same. The apparatus for circulating a carrier fluid includes two or more chambers maintained at different temperatures, each chamber having an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port), and an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port), wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.

Abstract translation: 提供一种用于循环具有两个或更多个室或部分的载体流体的装置，用于使用其的核酸的放大装置和含有该核酸的芯片的装置。 用于循环载体流体的装置包括保持在不同温度的两个或更多个室，每个室具有入口阀，该入口阀包含用于控制载体流体流入室（进气气压端口）的气动空气压力端口，以及出口 阀包含用于控制从腔室（出口气动空气压力端口）流出的载气的气动空气压力，其中腔室顺序地连接，使得一个室的出口阀连接到相邻室的入口阀 方向流体流动。

公开(公告)号：US20050202489A1

公开(公告)日：2005-09-15

申请号：US11071383

申请日：2005-03-03

Applicant: Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

Inventor： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

IPC: C12N15/09 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/38 ,  C12N15/00 ,  C12Q1/68 ,  G01N35/00 ,  G01N35/08 ,  C12P19/34

CPC classification number: G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

Abstract: Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

Abstract translation: 提供了用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

公开(公告)号：US20120088226A1

公开(公告)日：2012-04-12

申请号：US13001444

申请日：2009-06-22

Applicant: Geun-Bae Lim ,  Ji-Min Kahng ,  Tae-Hee Kang ,  Jin-Hwa Jung

Inventor： Geun-Bae Lim ,  Ji-Min Kahng ,  Tae-Hee Kang ,  Jin-Hwa Jung

IPC: C12Q1/70 ,  C12Q1/68 ,  C07H21/04 ,  C07H21/00 ,  C07H1/08

CPC classification number: C12N15/1017 ,  C12N15/1006

Abstract: The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel column that is provided inside the tube and filters impurities excluding an extraction target material.

Abstract translation: 核酸提取装置技术领域本发明涉及核酸提取装置，核酸提取装置可以包括在其一侧具有开放出口的管状管和设置在管内的水凝胶柱，并且除去除去提取目标之外的杂质 材料。

公开(公告)号：US20100252525A1

公开(公告)日：2010-10-07

申请号：US12741058

申请日：2008-03-12

Applicant: Dong-Seob Kim ,  Sang-Min Lee ,  Woon-Bong Hwang ,  Kun-Hong Lee ,  Geun-Bae Lim ,  Joon-Won Kim ,  Dong-Hyun Kim ,  Hyun-Chul Park

Inventor： Dong-Seob Kim ,  Sang-Min Lee ,  Woon-Bong Hwang ,  Kun-Hong Lee ,  Geun-Bae Lim ,  Joon-Won Kim ,  Dong-Hyun Kim ,  Hyun-Chul Park

IPC: C23F1/00

CPC classification number: C23F1/00 ,  C25D1/08 ,  C25D11/005 ,  C25D11/02

Abstract: The present invention relates to a three-dimensional structure manufacturing method for performing surface treatment processes, and a replication step to provide hydrophobicity on an external surface of the three-dimensional structure. In the manufacturing method, the hydrophobicity may be provided to the external surface of the three-dimensional structure, a high cost device required in the conventional MEMS process is not used, the manufacturing cost is reduced, and the manufacturing process is simplified. In addition, it has been difficult to provide the hydrophobicity on an external surface of a three-dimensional structure having a large surface due to a spatial limitation, but in the exemplary embodiment of the present invention, the hydrophobicity may be provided to the external surface of the three-dimensional structure having a large surface, such as a torpedo, a submarine, a ship, and a vehicle, without the spatial limitation.

Abstract translation: 本发明涉及一种用于进行表面处理工艺的三维结构制造方法以及在三维结构的外表面上提供疏水性的复制步骤。 在制造方法中，可以将疏水性提供给三维结构的外表面，不使用常规MEMS工艺中所需的高成本装置，制造成本降低，制造工艺简化。 此外，由于空间限制，难以在具有大表面的三维结构的外表面上提供疏水性，但是在本发明的示例性实施方案中，可以将疏水性提供给外表面 的具有大表面的三维结构，例如鱼雷，潜艇，船舶和车辆，没有空间限制。