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    Method for thermal cycling of microfluidic samples
    11.
    发明授权
    Method for thermal cycling of microfluidic samples 有权
    微流体样品的热循环方法

    公开(公告)号：US09168531B2

    公开(公告)日：2015-10-27

    申请号：US13429861

    申请日：2012-03-26

    Applicant: Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

    Inventor： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

    IPC: C12P19/34 ,  B01L7/00 ,  C12Q3/00 ,  B01L3/00 ,  G01N21/64 ,  G01N21/76

    CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12P19/34 ,  C12Q3/00 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/76

    Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.

    Abstract translation: 用于微流体装置的热循环仪包括控制器，其可操作以提供与散热器热连通的一系列电信号，散热器和加热元件，并可操作以从控制器接收一系列电信号。 热循环仪还包括与加热元件热连通的热卡盘。 热卡盘包括可操作以与微流体装置进行热接触的加热表面。 加热表面的特征在于每秒2.5摄氏度和5.5摄氏度/秒之间的温度升高速率，并且支撑微流体装置的第一部分的加热表面的第一部分与加热表面的第二部分之间的温度差 支撑微流体装置的第二部分小于0.25℃

    MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS
    12.
    发明申请
    MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS 有权
    微流化粒子分析系统

    公开(公告)号：US20140378352A1

    公开(公告)日：2014-12-25

    申请号：US14188660

    申请日：2014-02-24

    Applicant: Fluidigm Corporation

    Inventor： Antoine Daridon

    IPC: B01L3/00

    CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

    Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

    Abstract translation: 本发明提供了用于微流体操纵和/或检测诸如细胞和/或珠粒的装置，方法和试剂盒的系统。 本发明提供了用于微粒操作和/或分析颗粒例如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒的系统。 本发明还提供用于进行这些操作和分析的微流体机理。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机制可以以任何合适的顺序组合和/或在系统内任何适当次数使用。 因此，这些组合可以允许将粒子分类，培养，混合，处理和/或测定为单粒子，粒子的混合组，粒子阵列，非均匀粒子组和/或均匀粒子组，其中 其他，串联和/或并行。 此外，这些组合可以使微流体系统能够重复使用。 此外，这些组合可以允许在比以前可能的更短的时间尺度上测量颗粒对治疗的反应。 因此，本发明的系统可以允许广泛范围的细胞和颗粒测定，例如药物筛选，细胞特征化，研究研究和/或临床分析等，以缩小到微流体大小。 这种按比例缩小的测定可能使用较少的样品和试剂，可能较少的劳动密集型和/或可能比可比较的大流控测定更具信息性。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    16.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    颗粒人口的单粒子分析

    公开(公告)号：US20130323732A1

    公开(公告)日：2013-12-05

    申请号：US13899397

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/68 ,  B01D15/08

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 可以在颗粒处于分开的反应体积中时测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    Systems and methods for effecting a physical change in a biological sample
    17.
    发明授权
    Systems and methods for effecting a physical change in a biological sample 有权
    影响生物样品物理变化的系统和方法

    公开(公告)号：US08450095B2

    公开(公告)日：2013-05-28

    申请号：US12835822

    申请日：2010-07-14

    Applicant: Kyle W. Hukari ,  Jason A. A. West

    Inventor： Kyle W. Hukari ,  Jason A. A. West

    IPC: C12N13/00

    CPC classification number: C12N13/00 ,  C12M47/06

    Abstract: The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C.

    Abstract translation: 本发明一般涉及用于处理导致物理变化的生物样品的系统和方法，例如将两个分子一起反应以形成反应产物或用于裂解病毒或生物细胞用于使用生物测定系统进行分析。 因此，本发明涉及分解生物物种如病毒和细胞，以及从一种或多种反应性物质形成反应物。 样品的体积在约1微升至10毫升的范围内。 通过对样品施加压力和声能或热能来处理样品，其中获得的压力通常为至少24个大气压，并且样品的温度通常升高至至少50℃。

    Method and system for crystallization and X-ray diffraction screening
    18.
    发明授权
    Method and system for crystallization and X-ray diffraction screening 有权
    用于结晶和X射线衍射筛选的方法和系统

    公开(公告)号：US08422632B2

    公开(公告)日：2013-04-16

    申请号：US13168041

    申请日：2011-06-24

    Applicant: Brian Fowler ,  Andrew May

    Inventor： Brian Fowler ,  Andrew May

    IPC: G01N23/20

    CPC classification number: G01N23/20025

    Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

    Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

    NUCLEIC ACID ENCODING REACTIONS
    19.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 有权
    核酸编码反应

    公开(公告)号：US20130005585A1

    公开(公告)日：2013-01-03

    申请号：US13476911

    申请日：2012-05-21

    Applicant: Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C40B20/00 ,  C12Q1/68 ,  C40B30/04 ,  C12P19/34 ,  C40B50/06 ,  C40B40/06

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

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