公开(公告)号：US20050000900A1

公开(公告)日：2005-01-06

申请号：US10874103

申请日：2004-06-21

Applicant: Jiang Huang ,  Hou-Pu Chou ,  Marc Unger

Inventor： Jiang Huang ,  Hou-Pu Chou ,  Marc Unger

IPC: G01N30/38 ,  G01N30/52 ,  G01N30/60 ,  B01D15/08

CPC classification number: B01D15/08 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/387 ,  G01N2030/521 ,  Y10T137/86863 ,  Y10T436/2575

Abstract: The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

Abstract translation: 本发明涉及包含微流体输送系统和与流体输送系统流体连通的色谱柱的微流体色谱装置及其制造和使用方法。 优选地，色谱柱包括OTLC，PCLC或其组合。

公开(公告)号：US09643178B2

公开(公告)日：2017-05-09

申请号：US13439364

申请日：2012-04-04

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: B01L3/00 ,  C12Q1/68 ,  G01N35/00 ,  B01F13/00 ,  C40B60/14

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.

公开(公告)号：US09623413B2

公开(公告)日：2017-04-18

申请号：US12761917

申请日：2010-04-16

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C30B7/00 ,  C30B29/58 ,  C30B35/00 ,  B01L3/02 ,  B01L3/06

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

公开(公告)号：US08697363B2

公开(公告)日：2014-04-15

申请号：US12548132

申请日：2009-08-26

Applicant: Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

Inventor： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay. In certain embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a sample-specific nucleotide tag and a target nucleotide sequence. In some embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a first nucleotide tag linked to a target nucleotide sequence, which is linked to a second nucleotide tag. In either case, the tagged target nucleotide sequences from the S samples can be mixed to form an assay mixture and subsequently assayed.

Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。 在某些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括样品特异性核苷酸标签和靶核苷酸序列。 在一些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括与靶核苷酸序列连接的第一个核苷酸标签，其连接到第二个 核苷酸标签。 在任一情况下，可以将来自S样品的标记的靶核苷酸序列混合以形成测定混合物并随后测定。

公开(公告)号：US08475743B2

公开(公告)日：2013-07-02

申请号：US12422612

申请日：2009-04-13

Applicant: Geoffrey Facer ,  Brian Fowler ,  Emerson Cheung Quan ,  Marc Unger

Inventor： Geoffrey Facer ,  Brian Fowler ,  Emerson Cheung Quan ,  Marc Unger

IPC: B01L3/00 ,  F16K3/00 ,  F16K21/20 ,  G01N21/75 ,  F04B19/00 ,  G05D11/00 ,  F15C1/14 ,  F17D3/00

CPC classification number: F16K99/0015 ,  B01F5/02 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  B33Y80/00 ,  C12Q1/686 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2021/0346 ,  Y10T137/0329 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2559 ,  Y10T137/2655 ,  Y10T137/2688 ,  Y10T137/87249

Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.

公开(公告)号：US08440093B1

公开(公告)日：2013-05-14

申请号：US11546939

申请日：2006-10-11

Applicant: Hany Nassef ,  Geoff Facer ,  Marc Unger

Inventor： Hany Nassef ,  Geoff Facer ,  Marc Unger

IPC: C03C25/68 ,  G01N1/10

CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

公开(公告)号：US20130065773A1

公开(公告)日：2013-03-14

申请号：US13439364

申请日：2012-04-04

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12M1/40 ,  B05D7/02 ,  C40B60/12 ,  C12Q1/68 ,  C12Q1/04 ,  C40B30/04 ,  F16K24/00 ,  C12M1/34

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US08343442B2

公开(公告)日：2013-01-01

申请号：US12859176

申请日：2010-08-18

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：US08163492B2

公开(公告)日：2012-04-24

申请号：US12858364

申请日：2010-08-17

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12Q1/68

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US08105553B2

公开(公告)日：2012-01-31

申请号：US11043895

申请日：2005-01-25

Applicant: Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk

Inventor： Robert Grossman ,  Marc Unger ,  Phillip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk

IPC: C12Q1/68 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N35/00

CPC classification number: C12Q1/68 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/11

Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

Abstract translation: 本发明提供了微流体装置及其使用方法。 本发明还提供了用于使用微流体装置的装置和系统，分析在微流体装置中进行的反应，以及用于生成，存储，组织和分析使用微流体装置产生的数据的系统。 本发明还提供了使用和制造微流体系统和装置的方法，在一些实施方案中，它们可用于晶体形成。 在一个实施例中，一种装置包括具有其中具有一个或多个流体端口的压板面的压板。 流体端口在空间上对应于微流体装置表面上的一个或多个孔。 包括用于相对于压板保持微流体装置的平台，以及用于将压板压靠在微流体装置上的压板致动器，使得压板的至少一个流体端口被推压到一个井中以形成压力室 包括井和端口，使得当压力流体通过其中一个端口被引入或从压力室移除时，流体压力在其中改变。