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    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    347.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20170260512A1

    公开(公告)日：2017-09-14

    申请号：US15481858

    申请日：2017-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Ultra-fast nucleic acid sequencing device and a method for making and using the same
    348.
    发明授权
    Ultra-fast nucleic acid sequencing device and a method for making and using the same 有权

    公开(公告)号：US09758824B2

    公开(公告)日：2017-09-12

    申请号：US15231480

    申请日：2016-08-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jon R. Sauer ,  Bart J. Van Zeghbroeck

    IPC: H01L29/66 ,  C12Q1/68 ,  G01N33/487 ,  G01N27/447 ,  B82Y30/00

    CPC classification number: C12Q1/6869 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721

    Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.

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