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公开(公告)号：US11499181B2
公开(公告)日：2022-11-15
申请号：US16235996
申请日：2018-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686
Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US11060136B2
公开(公告)日：2021-07-13
申请号：US16447315
申请日：2019-06-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Benjamin J. Hindson
IPC: C12Q1/68 , C12Q1/6848 , C12Q1/6844 , C12Q1/6816 , C12Q1/686
Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
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公开(公告)号：US20190241947A1
公开(公告)日：2019-08-08
申请号：US16235996
申请日：2018-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, JR.
IPC: C12Q1/6858 , C12Q1/6806 , C12Q1/686 , C12Q1/6827
CPC classification number: C12Q1/6806 , C12Q1/6827 , C12Q1/683 , C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2535/125 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US10150786B2
公开(公告)日：2018-12-11
申请号：US15697313
申请日：2017-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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公开(公告)号：US20170362258A1
公开(公告)日：2017-12-21
申请号：US15697313
申请日：2017-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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Patent Agency Ranking
公开(公告)号：US20170175179A1
公开(公告)日：2017-06-22
申请号：US15452582
申请日：2017-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Benjamin J. Hindson
IPC: C12Q1/68
CPC classification number: C12Q1/6848 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , C12Q2527/125 , C12Q2563/159
Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
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公开(公告)号：US20170151558A1
公开(公告)日：2017-06-01
申请号：US15351354
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
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公开(公告)号：US09649635B2
公开(公告)日：2017-05-16
申请号：US15351331
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Kevin D. Ness , Benjamin J. Hindson , Donald A. Masquelier , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
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公开(公告)号：US09636682B2
公开(公告)日：2017-05-02
申请号：US15351335
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Kevin D. Ness , Benjamin J. Hindson , Donald A. Masquelier
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well. The device also may include a channel network having a first channel, a second channel, and a third channel that meet one another in a droplet-generation region. The system also may comprise a holder for the device. The system further may comprise an instrument configured to operatively receive an assembly including the device and the holder and to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel.
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公开(公告)号：US20150307919A1
公开(公告)日：2015-10-29
申请号：US14791111
申请日：2015-07-02
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Benjamin J. Hindson , Billy W. Colston, JR.
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , Y02A90/26
Abstract: Method of performing a multiplexed digital assay. The method may include (1) partitioning a sample containing at least two distinct targets into droplets; (2) amplifying the at least two distinct targets within the droplets; (3) detecting light from the droplets indicative of the presence or absence of each distinct target in each droplet; and (4) calculating a level of each distinct target based on the detected light.
Abstract translation: 执行复用数字测定的方法。 该方法可以包括（1）将含有至少两个不同目标的样品分成液滴; （2）在液滴内放大至少两个不同的目标; （3）检测来自液滴的光，指示每个液滴中每个不同目标的存在或不存在; 和（4）基于检测到的光来计算每个不同目标的水平。
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