公开(公告)号：US20100178655A1

公开(公告)日：2010-07-15

申请号：US12687018

申请日：2010-01-13

Applicant: Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。

公开(公告)号：US07749737B2

公开(公告)日：2010-07-06

申请号：US11929436

申请日：2007-10-30

Applicant: Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

Inventor： Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/68 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/686 ,  G01N27/453

Abstract: An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的M次.N基质微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US07666361B2

公开(公告)日：2010-02-23

申请号：US10818642

申请日：2004-04-05

Applicant: Lincoln McBride ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef ,  Geoff Facer ,  Yong Yi

Inventor： Lincoln McBride ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef ,  Geoff Facer ,  Yong Yi

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US07583853B2

公开(公告)日：2009-09-01

申请号：US10902494

申请日：2004-07-28

Applicant: Colin Jon Taylor ,  Gang Sun ,  Simant Dube

Inventor： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

IPC: G06K9/36

CPC classification number: G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

Abstract: A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

Abstract translation: 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

公开(公告)号：US20090142236A1

公开(公告)日：2009-06-04

申请号：US11929660

申请日：2007-10-30

Applicant: Marc Unger ,  Jiang Huang ,  Emerson Quan

Inventor： Marc Unger ,  Jiang Huang ,  Emerson Quan

IPC: B01J19/00

CPC classification number: B01L3/00 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: An M.times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的M.times.N矩阵微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US07476363B2

公开(公告)日：2009-01-13

申请号：US10837885

申请日：2004-05-02

Applicant: Marc Unger ,  Jiang Huang ,  Emerson Quan

Inventor： Marc Unger ,  Jiang Huang ,  Emerson Quan

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的MxN矩阵微流体装置，所述装置具有多个反应池，其通过形成在所述装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US07452726B2

公开(公告)日：2008-11-18

申请号：US10734963

申请日：2003-12-11

Applicant: Hou-Pu Chou ,  Antoine Daridon ,  Kevin Farrell ,  Brian Fowler ,  Yish-Hann Liau ,  Ian D. Manger ,  Hany Ramez Nassef ,  William Throndset

Inventor： Hou-Pu Chou ,  Antoine Daridon ,  Kevin Farrell ,  Brian Fowler ,  Yish-Hann Liau ,  Ian D. Manger ,  Hany Ramez Nassef ,  William Throndset

IPC: G01N35/00 ,  G01N33/48 ,  G01N1/10 ,  G01N15/06 ,  G01N33/00

CPC classification number: G01N35/00 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1456 ,  G01N15/1468 ,  G01N15/1484 ,  G01N2015/0288 ,  G01N2015/1075 ,  G01N2015/149 ,  G01N2015/1493 ,  Y10T436/11 ,  Y10T436/2575

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

Abstract translation: 本发明提供了用于微流体操纵和/或检测诸如细胞和/或珠粒的装置，方法和试剂盒的系统。 本发明提供了用于微粒操作和/或分析颗粒例如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒的系统。 本发明还提供用于进行这些操作和分析的微流体机理。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机制可以以任何合适的顺序组合和/或在系统内任何适当次数使用。 因此，这些组合可以允许将粒子分类，培养，混合，处理和/或测定为单粒子，粒子的混合组，粒子阵列，非均匀粒子组和/或均匀粒子组，其中 其他，串联和/或并行。 此外，这些组合可以使微流体系统能够重复使用。 此外，这些组合可以允许在比以前可能的更短的时间尺度上测量颗粒对治疗的反应。 因此，本发明的系统可以允许广泛范围的细胞和颗粒测定，例如药物筛选，细胞特征化，研究研究和/或临床分析等，以缩小到微流体大小。 这种按比例缩小的测定可能使用较少的样品和试剂，可能较少的劳动密集型和/或可能比可比较的大流控测定更具信息性。

公开(公告)号：US07442556B2

公开(公告)日：2008-10-28

申请号：US11302605

申请日：2005-12-13

Applicant: Ian David Manger ,  Cunsheng Casey Hao ,  Marc Alexander Unger

Inventor： Ian David Manger ,  Cunsheng Casey Hao ,  Marc Alexander Unger

IPC: G01N1/10 ,  B01D59/44

CPC classification number: G01N1/10 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N30/7266 ,  H01J49/0018 ,  H01J49/165 ,  Y10T436/117497 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: This invention provides microfluidic devices and methods for using the same. Microfluidic devices of the present invention comprises a first elastic layer, a fluid flow channel within the elastic layer; and a means for providing a fluid sample from the fluid flow channel to an analytical device. The present invention also provides an analytical apparatus comprising such a microfluidic device and an analytical device.

Abstract translation: 本发明提供了微流体装置及其使用方法。 本发明的微流体装置包括第一弹性层，弹性层内的流体流动通道; 以及用于将流体样品从流体流动通道提供到分析装置的装置。 本发明还提供一种包括这种微流体装置和分析装置的分析装置。

公开(公告)号：US20080257437A1

公开(公告)日：2008-10-23

申请号：US12144603

申请日：2008-06-23

Applicant: David N. Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

Inventor： David N. Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

IPC: F15C3/00

CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

Abstract: Microfabricated fluidic devices of the present invention include switches that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include high or low pressure sources. A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.

Abstract translation: 本发明的微型流体装置包括可以打开和关闭的开关，以响应于开关的门中的压力水平来允许或阻止流体通过通道的流动。 微制式流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。 逻辑门可以耦合在一起以形成锁存信号的触发器。 本发明还包括相对于第一腔室增加第二腔室中的压力的​​微制造流体压力倍增器。 本发明的微型流体装置还包括高压或低压源。 本发明的压力源包括通过单向阀联接到储存器的泵。 本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。

公开(公告)号：US20080223721A1

公开(公告)日：2008-09-18

申请号：US12018138

申请日：2008-01-22

Applicant: David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

Inventor： David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

IPC: G01N27/26

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y80/00 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract translation: 新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。 将第一溶液引入与反应室流体连通的流动通道的段中。 第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入该室。 然后可以分离室，并且可以启动和/或检测室内的反应。 遗传分析的高通量方法可以比以前更高的精度进行。