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50 条记录 (耗时 0.061 秒)

    Reagent for nucleic acid typing by primer extension
    45.
    发明授权
    Reagent for nucleic acid typing by primer extension 有权
    通过引物延伸进行核酸分型的试剂

    公开(公告)号：US06537748B1

    公开(公告)日：2003-03-25

    申请号：US09258133

    申请日：1999-02-26

    申请人： Philip Goelet ,  Michael R. Knapp ,  Stephen Anderson

    发明人： Philip Goelet ,  Michael R. Knapp ,  Stephen Anderson

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2535/125 ,  C12Q2535/101 ,  C12Q2563/107 ,  C12Q2537/143 ,  C12Q2525/121

    摘要： This invention concerns a reagent composition comprising at least two different terminators of a nucleic acid template-dependent, primer extension reaction. This invention also concerns a method for determining the identity of a nucleotide base at a specific position in a nucleic acid of interest. This invention further concerns a method for determining the presence or absence of a particular nucleotide sequence in a sample of nucleic acids. This invention further concerns a method for identifying different alleles in a sample containing nucleic acids. This invention further concerns a method for determining the genotype of an organism at one or more particular genetic loci.

    摘要翻译： 本发明涉及包含核酸模板依赖性引物延伸反应的至少两种不同终止子的试剂组合物。 本发明还涉及用于确定感兴趣的核酸中特定位置的核苷酸碱基的身份的方法。 本发明还涉及用于确定核酸样品中特定核苷酸序列的存在或不存在的方法。 本发明还涉及用于鉴定含有核酸的样品中的不同等位基因的方法。 本发明还涉及用于确定一个或多个特定遗传基因座上的生物体的基因型的方法。

    Microfluidic systems including pipettor elements
    46.
    发明授权
    Microfluidic systems including pipettor elements 有权
    微流体系统包括移液器元件

    公开(公告)号：US06482364B2

    公开(公告)日：2002-11-19

    申请号：US09848717

    申请日：2001-05-03

    申请人： J. Wallace Parce ,  Michael R. Knapp

    发明人： J. Wallace Parce ,  Michael R. Knapp

    IPC分类号： B01L302

    CPC分类号： B01F13/0071 ,  B01F13/0084 ,  B01F13/0086 ,  B01J19/0093 ,  B01J2219/00891 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  F04B17/00 ,  F04B19/006 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  H02N11/006 ,  Y10S366/02 ,  Y10T436/113332 ,  Y10T436/2575

    摘要： The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.

    摘要翻译： 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域运输，靠近高离子浓度的间隔物材料区域，其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化，以避免混合材料。 使用这些技术，产生与微流体系统相容的电吸移器，使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压，因为材料通过将通道分成具有正表面电荷和负表面电荷的部分，以及在两部分之间的第三电极，或者通过在运输之后电泳材料的扩散而传输通过微流体系统的通道 一个频道

    Electropipettor and compensation means for electrophoretic bias
    47.
    发明授权
    Electropipettor and compensation means for electrophoretic bias 有权
    电泳机和电泳偏倚的补偿手段

    公开(公告)号：US06287520B1

    公开(公告)日：2001-09-11

    申请号：US09488801

    申请日：2000-01-21

    申请人： J. Wallace Parce ,  Michael R. Knapp

    发明人： J. Wallace Parce ,  Michael R. Knapp

    IPC分类号： B01L302

    CPC分类号： B01F13/0071 ,  B01F13/0084 ,  B01F13/0086 ,  B01J19/0093 ,  B01J2219/00891 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  F04B17/00 ,  F04B19/006 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  H02N11/006 ,  Y10S366/02 ,  Y10T436/113332 ,  Y10T436/2575

    摘要： The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.

    摘要翻译： 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域运输，靠近高离子浓度的间隔物材料区域，其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化，以避免混合材料。 使用这些技术，产生与微流体系统相容的电吸移器，使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压，因为材料通过将通道分成具有正表面电荷和负表面电荷的部分，以及两部分之间的第三电极，或者通过在运输之后通过电泳材料的扩散而传输通过微流体系统的通道 一个频道

    Electropipettor and compensation means for electrophoretic bias
    48.
    发明授权
    Electropipettor and compensation means for electrophoretic bias 失效
    电泳机和电泳偏倚的补偿手段

    公开(公告)号：US5880071A

    公开(公告)日：1999-03-09

    申请号：US760446

    申请日：1996-12-06

    申请人： J. Wallace Parce ,  Michael R. Knapp

    发明人： J. Wallace Parce ,  Michael R. Knapp

    IPC分类号： B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  F04B17/00 ,  F04B19/00 ,  G01N27/447 ,  H02N11/00 ,  G01N27/26

    CPC分类号： B01F13/0071 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  F04B17/00 ,  F04B19/006 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  H02N11/006 ,  B01F13/0084 ,  B01F13/0086 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L3/021 ,  B01L3/502753

    摘要： The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.

    摘要翻译： 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域内运输，靠近高离子浓度的间隔物材料区域，其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化，以避免混合材料。 使用这些技术，产生与微流体系统相容的电吸移器，使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压，因为材料通过将通道分成具有正表面电荷和负表面电荷的部分，以及两部分之间的第三电极，或者通过在运输之后电泳材料的扩散而传输通过微流体系统的通道 一个频道

    Method for immobilizing nucleic acid molecules
    49.
    发明授权
    Method for immobilizing nucleic acid molecules 失效
    固定核酸分子的方法

    公开(公告)号：US5610287A

    公开(公告)日：1997-03-11

    申请号：US341148

    申请日：1994-11-16

    申请人： Theo Nikiforov ,  Michael R. Knapp

    发明人： Theo Nikiforov ,  Michael R. Knapp

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H21/04 ,  C12N11/08 ,  C12N15/00

    CPC分类号： C12Q1/6834 ,  C12N15/1006

    摘要： Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an --OH, --C.dbd.O or --COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.

    摘要翻译： 在含有-OH，-C = O或-COOH亲水基团的亲水性聚苯乙烯固体载体上或在玻璃固体载体上，在盐或阳离子洗涤剂存在下，合成核酸分子非共价固定。 将载体与含有合成核酸和阳离子洗涤剂或盐的pH约6至约8的溶液接触。 优选地，阳离子洗涤剂是浓度为约30mM至约100mM的1-乙基-3-（3'-二甲基氨基丙基）-1,3-碳二亚胺氯化物或浓度为约50mM至约150mM的辛基二甲基胺盐酸盐 。 该盐优选浓度为约50mM至约250mM的NaCl。 当洗涤剂为1-乙基-3-（3'-二甲基氨基丙基）-1,3-碳二亚胺氯化物时，使用玻璃载体或亲水性聚苯乙烯载体。 当使用NaCl或辛基二甲胺盐酸盐时，载体是亲水性聚苯乙烯。 固定后，含有固定化核酸的载体可以用含有非离子型洗涤剂的水溶液洗涤。 固定核酸可用于核酸杂交测定，核酸测序和基因组多态性分析。