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公开(公告)号：US08613889B2
公开(公告)日：2013-12-24
申请号：US11639531
申请日：2006-12-15
CPC分类号： B01L3/502784 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to droplet-based washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴洗涤。 根据一个实施方案，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供与液滴接触的表面，所述液滴包含起始浓度和起始浓度 该物质并具有起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，所述液滴包括：（i）与所述物质接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US08562807B2
公开(公告)日：2013-10-22
申请号：US12747231
申请日：2008-12-10
申请人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译： 提供用于进行液滴操作的滴液致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括基板，其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号：US07998436B2
公开(公告)日：2011-08-16
申请号：US11839774
申请日：2007-08-16
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.
摘要翻译： 提供多孔液滴致动器，系统和方法。 根据一个实施例，提供了一种衬底，其包括：（a）用于引入一种或多种试剂和/或样品的一个或多个输入端口; （b）一系列加工井; 和（c）液滴传输路径的网络，其包括提供从每个输入端口到所述一个或多个处理井中的每一个的直接或间接液滴传输的路径。 还提供了与其相关的不同液滴致动器和系统。
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公开(公告)号：US20110104816A1
公开(公告)日：2011-05-05
申请号：US12990815
申请日：2009-05-04
申请人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen Eckhardt , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen Eckhardt , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/00
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要： The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
摘要翻译： 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号：US20110104747A1
公开(公告)日：2011-05-05
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/40
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译： 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法，其中包括将珠粒集中在液滴中的方法，所述方法包括：（a）提供液滴致动器，其包括：（i） 内部液滴操作量; 和（ii）内部容积外部的储存器; （iii）建立在从储存器延伸到内部容积中的液体路径中的液滴; （b）在储存器中的液滴部分中提供磁响应珠; （c）通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和（d）在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号：US07901947B2
公开(公告)日：2011-03-08
申请号：US11639566
申请日：2006-12-15
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译： 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。
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公开(公告)号：US07815871B2
公开(公告)日：2010-10-19
申请号：US11639664
申请日：2006-12-15
CPC分类号： B41J2/125 , B01L3/502792 , B01L2200/143 , B01L2200/147 , B01L2300/0645 , B01L2300/0654 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B41J2/17509 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to a droplet microactuator system. According to one embodiment, the droplet microactuator system includes: (a) a droplet microactuator configured to conduct droplet operations; (b) a magnetic field source arranged to immobilize magnetically responsive beads in a droplet during droplet operations; (c) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator; and (d) one or more processors electronically coupled to the droplet microactuator and programmed to control electrowetting-mediated droplet operations on the droplet actuator and process electronic signals from the sensor.
摘要翻译： 液滴微致动器系统技术领域本发明涉及液滴微致动器系统。 根据一个实施例，液滴微致动器系统包括：（a）构造成进行液滴操作的液滴微致动器; （b）磁场源，布置成在液滴操作期间将磁响应珠固定在液滴中; （c）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性; 和（d）电子耦合到液滴微致动器的一个或多个处理器，并被编程为控制液滴致动器上的电润湿介导的液滴操作并处理来自传感器的电子信号。
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公开(公告)号：US20100236929A1
公开(公告)日：2010-09-23
申请号：US12681862
申请日：2008-10-16
CPC分类号： B01L3/50273 , B01L2200/0673 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2300/161 , B01L2400/0427
摘要： A droplet actuator with arrays of electrodes electrically coupled to a number of controllable voltage sources that is less than the number of electrodes. A method of defining partitions for pin layouts in a droplet actuator for a specific assay, the method including: defining droplet traces for the assay; and defining a guard ring along the traces. Other methods, systems, droplet actuators, and algorithms are also provided.
摘要翻译： 液滴致动器，其具有电耦合到少于电极数量的多个可控电压源的电极阵列。 一种用于针对特定测定的液滴致动器中的针布局定义分隔的方法，所述方法包括：定义用于测定的液滴迹线; 并沿轨迹定义保护环。 还提供了其他方法，系统，液滴致动器和算法。
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公开(公告)号：US20100221713A1
公开(公告)日：2010-09-02
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20100126860A1
公开(公告)日：2010-05-27
申请号：US12531794
申请日：2008-08-11
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译： 提供制造用于液滴致动器操作的印刷电路板的替代方法。 在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。 该方法还包括在第一和第二金属层之间形成连接。 还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。
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