公开(公告)号：US20130045876A1

公开(公告)日：2013-02-21

申请号：US13579666

申请日：2011-02-21

Applicant: Sanket Goel ,  Min Gong ,  Abdur Rub Abdur Rahman ,  Shihui Foo

Inventor： Sanket Goel ,  Min Gong ,  Abdur Rub Abdur Rahman ,  Shihui Foo

IPC: C12Q1/68 ,  C40B60/12 ,  C40B30/00 ,  C12M1/34

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q2565/301

Abstract: The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.

公开(公告)号：US08343443B2

公开(公告)日：2013-01-01

申请号：US12935576

申请日：2008-06-23

Applicant: Jackie Y. Ying ,  Guolin Xu ,  Hock Ming Jeremy Loh

Inventor： Jackie Y. Ying ,  Guolin Xu ,  Hock Ming Jeremy Loh

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: G01N35/1095 ,  B01L3/5027 ,  B01L3/50851 ,  B01L2200/026 ,  B01L2200/04 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0672 ,  B01L2300/0809 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/0487 ,  Y10T137/0391 ,  Y10T436/11 ,  Y10T436/2575

Abstract: A micro-fluidic device comprises a body. The body defines pneumatic ports, chambers for receiving liquids, and a connecting conduit. Each port is sealed with a seal and is shaped to couple to a pneumatic conduit through the seal. At least some of the chambers each have a top opening and a bottom opening. The top openings are in fluid communication with corresponding ports. The bottom openings are in fluid communication with one another through the connecting conduit, which is above the bottom openings. Selective application of pneumatic pressures to the chambers through the pneumatic conduits can transfer a liquid from one chamber to another through the connecting conduit, for example, for processing bio-samples within the device.

Abstract translation: 微流体装置包括主体。 主体定义气动端口，用于接收液体的腔室和连接导管。 每个端口用密封件密封，并且成形为通过密封件连接到气动导管。 至少一些室各自具有顶部开口和底部开口。 顶部开口与相应的端口流体连通。 底部开口通过位于底部开口上方的连接导管彼此流体连通。 通过气动导管将气压压力选择性地施加到腔室可以将液体从一个腔室传送到另一个腔室，例如通过连接导管，用于处理装置内的生物样品。

公开(公告)号：US20120183453A1

公开(公告)日：2012-07-19

申请号：US13405477

申请日：2012-02-27

Applicant: Dieter BOEHM ,  Wolfgang ETTIG ,  Peter MAHLER ,  Juergen SCHRAUT ,  Roland WOHNER

Inventor： Dieter BOEHM ,  Wolfgang ETTIG ,  Peter MAHLER ,  Juergen SCHRAUT ,  Roland WOHNER

IPC: B01L3/00

CPC classification number: B01L3/0206 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/042 ,  B01L2300/06 ,  B01L2400/0478

Abstract: A bottle top dispenser for handling liquids having a piston-cylinder arrangement in an outside housing. At least one actuating button (49) that can be pressed downwards is arranged on the upper side of the outside housing. The outside housing (2) can be provided with ventilation openings (48) that can be hidden beneath the actuating button (49). The bottle arrangement is also characterized by a specific arrangement of a sensor system (65) in relation to a measuring strip (64) on a piston rod (28). The path measuring signal can be evaluated by means of a magnetoresistive sensor system, especially such a system based on the AMR effect. The arrangement of the measuring strip (64) in relation to the longitudinal central axis of the piston rod (28) is of considerable importance.

Abstract translation: 一种用于处理在外部壳体中具有活塞 - 气缸装置的液体的瓶顶分配器。 至少一个能够被向下按压的致动按钮（49）布置在外壳的上侧。 外壳体（2）可设置有可隐藏在致动按钮（49）下方的通气开口（48）。 瓶装置的特征还在于传感器系统（65）相对于活塞杆（28）上的测量条（64）的具体布置。 路径测量信号可以通过磁阻传感器系统来评估，特别是基于AMR效应的系统。 测量条（64）相对于活塞杆（28）的纵向中心轴线的布置是非常重要的。

公开(公告)号：US20120058571A1

公开(公告)日：2012-03-08

申请号：US13222450

申请日：2011-08-31

Applicant: Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

Inventor： Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

IPC: G01N1/00 ,  B01L3/02

CPC classification number: B01L3/022 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2300/14 ,  B01L2400/0442 ,  B01L2400/0487 ,  Y10T436/2575

Abstract: In one aspect, the present invention provides methods, devices, and systems for ensuring that multiple components of a mixture are fully mixed in a continuous flow microfluidic system while ensuring that mixing between segments flowing through the chip is minimized. In some embodiments, the present invention includes mixing fluids in a droplet maintained at the tip of a pipette before the mixture is introduced to the microfluidic device. In another aspect, the present invention provides a pipette tip having a ratio of an outside diameter to an inside diameter that provides sufficient surface area for a droplet comprising up to the entire volume of the liquid to suspend from the pipette tip intact. In yet another aspect, the present invention provides methods, devices, and systems for delivering a reaction mixture to a microfluidic chip comprising a docking receptacle, an access tube and a reservoir.

Abstract translation: 一方面，本发明提供用于确保混合物的多个组分在连续流微流体系统中完全混合的方法，装置和系统，同时确保流过芯片的区段之间的混合最小化。 在一些实施方案中，本发明包括在将混合物引入微流体装置之前将保持在移液管尖端的液滴混合流体。 在另一方面，本发明提供了一种移液管尖端，其具有外径与内径之比，其为包含液体的整个体积的液滴提供足够的表面面积，从而完全悬浮于移液管尖端。 在另一方面，本发明提供用于将反应混合物输送到微流控芯片的方法，装置和系统，所述微流控芯片包括对接插座，进入管和储存器。

公开(公告)号：US20120028844A1

公开(公告)日：2012-02-02

申请号：US13195918

申请日：2011-08-02

Applicant: Pierre F. Indermuhle

Inventor： Pierre F. Indermuhle

IPC: C40B60/08

CPC classification number: G01N33/5304 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0874 ,  B01L2400/0406

Abstract: Multiplex binding assay assemblies are disclosed. The assemblies generally include at least one assay bar that includes a top side, a bottom side, and at least one well accessible from the top side of the assay bar. Each well includes a side surface, a bottom surface, an open top end, and at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom surface of the assay bar. The assemblies further include a dispenser bar that is adapted to be positioned adjacent to the top side of the assay bar, which includes one or more reservoirs that are configured to provide one or more reagents to the at least one secondary container located in each well of the assay bar.

Abstract translation: 公开了多重结合测定组件。 组件通常包括至少一个测定条，其包括顶侧，底侧和至少一个可从测定条的顶侧接近的测定条。 每个井包括侧表面，底表面，敞开的顶端和至少一个次级容器，每个次级容器包括毛细管，（i）从井的内部体积内的位置开始，以及（ii） 在测定条的底表面下方的位置结束。 组件还包括分配杆，其适于邻近测定条的顶侧定位，该分配杆包括一个或多个储存器，其被配置为向位于每个孔的每个孔中的至少一个次级容器提供一种或多种试剂 试验条。

公开(公告)号：US08105554B2

公开(公告)日：2012-01-31

申请号：US11078196

申请日：2005-03-11

Applicant: Tanya S. Kanigan ,  Steve Smith ,  John Linton ,  Robert Hess ,  Karl Yoder ,  Colin Brennan

Inventor： Tanya S. Kanigan ,  Steve Smith ,  John Linton ,  Robert Hess ,  Karl Yoder ,  Colin Brennan

IPC: B01L3/00

CPC classification number: B01L3/5085 ,  B01L3/0241 ,  B01L3/0244 ,  B01L3/5025 ,  B01L3/50857 ,  B01L3/563 ,  B01L2200/021 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0636 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2300/161 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/0318 ,  Y10T137/1624 ,  Y10T137/206 ,  Y10T436/2525

Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

Abstract translation: 提供了用于将微流体样品存储在纳升级样品芯片中的界面。 流体通道结构从样品阱阵列向选定的样品阱子集提供流体通路区域。 流体引入机构将样品流体引入流体存取区域，使得所选择的子集中的样品孔填充有样品流体，而未选择的样品池填充有样品流体。

公开(公告)号：US08089623B2

公开(公告)日：2012-01-03

申请号：US12543459

申请日：2009-08-18

Applicant: H. Pin Kao ,  Ian A. Harding ,  Achim Karger ,  Mark F. Oldham ,  Omead Ostadan ,  Greg Young

Inventor： H. Pin Kao ,  Ian A. Harding ,  Achim Karger ,  Mark F. Oldham ,  Omead Ostadan ,  Greg Young

IPC: G01N31/00 ,  G01J1/10

CPC classification number: G01N35/028 ,  B01L3/0293 ,  B01L3/5025 ,  B01L3/50851 ,  B01L3/50853 ,  B01L3/563 ,  B01L7/52 ,  B01L9/50 ,  B01L9/523 ,  B01L2200/021 ,  B01L2200/025 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2300/022 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2035/00366 ,  H04N5/361 ,  H04N5/367 ,  Y10T436/10

Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.

Abstract translation: 用于使用设置在多个反应室中的至少一个中的参考标准或非荧光物质的仪器的输出正常化的方法。 该方法包括收集和分析与参考标准或非荧光物质相关联的信号以确定归一化偏差。 然后将归一化偏压施加到从多个反应室的其余部分收集的数据信号。

公开(公告)号：US20110319300A1

公开(公告)日：2011-12-29

申请号：US13229208

申请日：2011-09-09

Applicant: Ian Hunter ,  Colin J.H. Brenan ,  Tanya S. Kanigan

Inventor： Ian Hunter ,  Colin J.H. Brenan ,  Tanya S. Kanigan

IPC: C40B60/10 ,  B23K26/38 ,  C40B60/14

CPC classification number: B01L3/0293 ,  B01F13/0071 ,  B01F13/0084 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00373 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00549 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L9/523 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0845 ,  B01L2400/022 ,  C40B60/14 ,  C40B70/00 ,  G01N30/466 ,  G01N30/6095 ,  G01N30/82 ,  G01N35/00069 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T83/0481 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface.

Abstract translation: 一种用于装载多个不同样本容器的方法和阵列填充系统，所述样本容器包括整体结构。 每个容器的特征在于亲水表面，并且容器由疏水表面分开。 该系统具有能够保持液体并适于运动的液体转移装置，以通过将液体拖过疏水表面而使保持在液体转移装置中的液体与阵列的连续容器顺序连通。

公开(公告)号：US20110312535A1

公开(公告)日：2011-12-22

申请号：US13127240

申请日：2009-10-24

Applicant: Jens Göhring ,  Stefan Heydenhauss ,  Friedrich Menges ,  Reiner Spolaczyk ,  Jana Lepschi

Inventor： Jens Göhring ,  Stefan Heydenhauss ,  Friedrich Menges ,  Reiner Spolaczyk ,  Jana Lepschi

IPC: C40B30/10 ,  B65B3/04 ,  C40B40/04

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2300/04 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2300/1827 ,  B01L2400/0481 ,  G01N21/0332 ,  G01N21/11 ,  G01N2021/0325 ,  G01N2021/6439 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333

Abstract: The cartridge according to the invention for analysing biological samples comprises: a reaction chamber and a biochip mounted in the reaction chamber, a filling nozzle connected so as to communicate with the reaction chamber, and a compensation chamber connected so as to communicate with the reaction chamber, wherein the reaction chamber, the compensation chamber and all lines connected thereto form a chamber sealed as far as the filling nozzle, wherein the filling nozzle forms a free passage to the reaction chamber from outside the cartridge, and a stopper is provided which fits positively and tightly in the filling nozzle in such a way that, when pressed in over a certain distance, fluid is displaced from the filling nozzle towards the reaction chamber.

Abstract translation: 根据本发明的用于分析生物样品的盒包括：反应室和安装在反应室中的生物芯片，连接成与反应室连通的填充喷嘴和连接成与反应室连通的补偿室 其中，所述反应室，所述补偿室和与其连接的所有管线形成密封到所述填充喷嘴的腔室，其中所述填充喷嘴从所述筒外部形成到所述反应室的自由通道， 并且以这样的方式紧密地在填充喷嘴中，当被压入一定距离时，流体从填充喷嘴朝向反应室移位。

公开(公告)号：US20110220239A1

公开(公告)日：2011-09-15

申请号：US13049686

申请日：2011-03-16

Applicant: Mark T. REED ,  Albert L. Carrillo ,  Ian A. Harding

Inventor： Mark T. REED ,  Albert L. Carrillo ,  Ian A. Harding

IPC: B65B3/04 ,  B01L3/00

CPC classification number: G01N35/028 ,  B01L3/0293 ,  B01L3/5025 ,  B01L3/50851 ,  B01L3/50853 ,  B01L3/563 ,  B01L7/52 ,  B01L9/50 ,  B01L9/523 ,  B01L2200/021 ,  B01L2200/025 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2300/022 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N21/274 ,  G01N21/6452

Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.

Abstract translation: 一种用于填充微板的填充装置。 微量培养板可以包括多个孔，每个孔的尺寸适于接受测定。 基底可以包括第一表面和相对的第二表面，用于接收设置在第一表面上的测定的第一测定输入端口，延伸穿过基底的多个分期毛细血管，以及第一多个微流体通道，其将第一测定 输入端口与多个分段毛细管中的至少一个。 多个分级毛细管中的每一个可以包括入口和出口，并且其大小适于接收测定。