公开(公告)号：US09175255B2

公开(公告)日：2015-11-03

申请号：US13583746

申请日：2011-03-09

Applicant: Chikashi Nakamura

Inventor： Chikashi Nakamura

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M47/04

Abstract: A method and apparatus for discriminating and separating only specific cells in cell samples is provided. The cell separation operation includes inserting a needle-shaped body, on which a substance that selectively binds to a marker substance within each target cell has been immobilized, into each of the cell samples on a substrate, thereby causing the marker substance within each target cell in the cell samples to bind to the needle-shaped body through the substance that selectively binds to the marker substance, and then, pulling up the needle-shaped body. The binding force between the needle-shaped body and each target cell when the marker substance within each target cell has bound to the needle-shaped body is made greater than an adhesion force of each of the cell samples to the substrate, so that only target cells each containing the marker substance are pulled up and separated from the substrate.

Abstract translation: 提供了用于区分和分离细胞样品中特定细胞的方法和装置。 细胞分离操作包括将在其上固定有选择性结合每个靶细胞内的标记物质的物质的针状体插入到基底上的每个细胞样品中，从而在每个靶细胞内引起标记物质 在细胞样品中通过选择性结合标记物质的物质结合针状体，然后拉起针状体。 当每个靶细胞内的标记物质与针状体结合时，针状体与每个靶细胞之间的结合力大于每个细胞样品对基底的粘附力，使得仅靶 将每个含有标记物质的细胞从基质中拉起并分离。

公开(公告)号：US09174222B2

公开(公告)日：2015-11-03

申请号：US13232781

申请日：2011-09-14

Applicant: Lotien Richard Huang ,  Thomas Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

Inventor： Lotien Richard Huang ,  Thomas Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

IPC: B01L3/00 ,  B03C1/32 ,  B03C1/30 ,  C12M1/00 ,  G01N33/50 ,  G01N1/40 ,  G01N35/00

CPC classification number: B03C1/30 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0472 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/32 ,  B03C2201/18 ,  B33Y80/00 ,  C12M47/04 ,  C12M47/06 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/5044 ,  G01N2035/00237 ,  Y10T137/0318 ,  Y10T137/8593 ,  Y10T436/25 ,  Y10T436/25375

Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Abstract translation: 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。

公开(公告)号：US09174212B2

公开(公告)日：2015-11-03

申请号：US14209046

申请日：2014-03-13

Applicant: CYTOVERA INC.

Inventor： Lotien R. Huang

IPC: C12M1/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/00 ,  C12Q1/04 ,  G01N1/30 ,  G01N33/48 ,  C12M1/34 ,  G01N15/06 ,  G01N21/00 ,  B01D24/00 ,  B01L3/00 ,  G01N15/10

CPC classification number: B01L3/502753 ,  B01L3/5021 ,  B01L3/50255 ,  B01L2300/042 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0409 ,  B01L2400/049 ,  B33Y80/00 ,  C12M47/04 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1087

Abstract: Embodiments of the present disclosure feature a filtration system comprising a filtration module for particle filtration and methods of using the device for the isolation of particles (e.g., viable cells). Advantageously, embodiments of the device provide for the high throughput filtration of large volumes of sample while preserving cell viability and providing high yields.

Abstract translation: 本公开的实施方案的特征在于包括用于颗粒过滤的过滤模块和使用该装置分离颗粒（例如活细胞）的方法的过滤系统。 有利地，该装置的实施例提供大量样品的高通量过滤，同时保持细胞存活力并提供高产率。

公开(公告)号：US20150292995A1

公开(公告)日：2015-10-15

申请号：US14747837

申请日：2015-06-23

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Manjiri T. Kshirsagar ,  Kurt J. Halverson ,  Raj Rajagopal

IPC: G01N1/40

CPC classification number: G01N1/4077 ,  C12M47/04 ,  C12Q1/24 ,  G01N1/405 ,  G01N33/1866 ,  G01N2001/4088

Abstract: Kits and systems for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the kit including concentration agent; and a system for isolating microorganisms from a sample.

Abstract translation: 用于从样品中分离微生物的试剂盒和系统，样品包括样品基质和微生物，试剂盒包括浓缩剂; 以及用于从样品中分离微生物的系统。

公开(公告)号：US09149569B2

公开(公告)日：2015-10-06

申请号：US13108871

申请日：2011-05-16

Applicant: Robert J. Hariri

Inventor： Robert J. Hariri

IPC: C12N5/071 ,  A61M1/02 ,  C12N5/073 ,  C12N5/0775 ,  A61K35/12 ,  A61K48/00

CPC classification number: C12N5/0605 ,  A01N1/021 ,  A61K35/12 ,  A61K35/50 ,  A61K48/00 ,  A61K2035/124 ,  A61M1/02 ,  A61M2202/0462 ,  A61M2210/1458 ,  A61M2210/1466 ,  C12M29/10 ,  C12M47/04 ,  C12N5/0603 ,  C12N5/0647 ,  C12N5/0668 ,  C12N2509/00 ,  C12N2510/02 ,  C12N2533/90

Abstract: The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.

Abstract translation: 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。

公开(公告)号：US09144583B2

公开(公告)日：2015-09-29

申请号：US11789188

申请日：2007-04-23

Applicant: Gregory D. Ariff ,  Thomas Cannon ,  Jennifer L. Case ,  Christian L. Haller ,  Paul Kosnik ,  Charles P. Luddy ,  Craig A. Mauch ,  Erik Vossman ,  Stuart K. Williams

Inventor： Gregory D. Ariff ,  Thomas Cannon ,  Jennifer L. Case ,  Christian L. Haller ,  Paul Kosnik ,  Charles P. Luddy ,  Craig A. Mauch ,  Erik Vossman ,  Stuart K. Williams

IPC: A01N1/00 ,  A61K35/00 ,  A61P43/00 ,  C12M1/00 ,  A61K35/12

CPC classification number: C12M47/04 ,  A61K35/12 ,  C12M45/05 ,  C12M45/06 ,  C12M45/09

Abstract: The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.

Abstract translation: 本发明提供了用于支持各种细胞疗法和组织工程方法的自动化装置。 本发明提供了一种能够从用于细胞疗法和/或组织工程的组织样品中分离细胞的自动细胞分离装置。 细胞分离装置可以与诸如细胞收集装置和/或Sodding装置的补充装置组合使用以支持各种疗法。 自动化装置包括介质和组织解离化学储存器，过滤器，细胞分离器和通过支撑移植物基质或其它血管内装置的移植室的灌注流动回路。 本发明还提供了使用本文所述装置制备的组织移植物和细胞样品在包括血管重建，组织和器官的再血管重建和重建以及治疗和预防疾病的多种疗法中的方法。

公开(公告)号：US20150268029A1

公开(公告)日：2015-09-24

申请号：US14438201

申请日：2013-11-05

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Amy Rowat ,  David J. Hoelzle ,  Clara Chan

IPC: G01B7/16 ,  G01L1/14 ,  G01N27/22

CPC classification number: G01B7/22 ,  B01L3/5027 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12M47/04 ,  G01L1/14 ,  G01N27/22 ,  G01N29/022 ,  G01N29/036 ,  G01N29/222 ,  G01N2291/02466 ,  G01N2291/02827

Abstract: This invention provides methods and devices for the high-throughput characterization of the mechanical properties of cells or particles. In certain embodiments the devices comprise a micro fluidic channel comprising: an oscillating element on a first side of said channel; and a detecting element on a second side of said channel opposite said oscillating element, wherein said detecting element is configured to detect a force transmitted through a cell or microparticle by said oscillating element. In certain embodiments the devices comprise a microfluidic channel comprising an integrated oscillator and sensor element on one first side of said channel, wherein said sensor is configured to detect a force transmitted through a cell or microparticle by said oscillator.

Abstract translation: 本发明提供用于高通量表征细胞或颗粒的机械性能的方法和装置。 在某些实施例中，装置包括微流体通道，其包括：在所述通道的第一侧上的振荡元件; 以及在所述通道的与所述振荡元件相对的第二侧上的检测元件，其中所述检测元件被配置为检测由所述振荡元件传递通过电池或微粒的力。 在某些实施例中，装置包括微流体通道，其包括在所述通道的一个第一侧上的集成振荡器和传感器元件，其中所述传感器被配置为检测由所述振荡器传递通过电池或微粒的力。

公开(公告)号：US20150231641A1

公开(公告)日：2015-08-20

申请号：US14700186

申请日：2015-04-30

Applicant: Carlo Tremolada

Inventor： Carlo Tremolada

IPC: B02C19/00 ,  A61K35/35 ,  A61M5/32

CPC classification number: A61M19/00 ,  A61B17/34 ,  A61K35/35 ,  A61M1/007 ,  A61M5/002 ,  A61M5/315 ,  A61M5/3286 ,  A61M2202/08 ,  A61M2202/09 ,  B02C19/0056 ,  B08B3/102 ,  C12M45/02 ,  C12M47/04 ,  C12N5/0653

Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.

公开(公告)号：US20150218506A1

公开(公告)日：2015-08-06

申请号：US14610613

申请日：2015-01-30

Applicant: DSM IP ASSETS B.V.

Inventor： JOHN E. NASH ,  WILLIAM T. FISHER ,  GINO BRADICA

IPC: C12M1/00 ,  C12N5/0775

CPC classification number: C12M47/04 ,  B04B3/00 ,  B04B7/12 ,  C12M47/02 ,  C12N5/0667

Abstract: A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

Abstract translation: 提供了用于使生物混合物（例如脂肪组织）的组分的上浆和分离的离心机装置。 该装置通过离心使得组织通过网状元件或定径螺旋或挤压元件来提供组织中的纤维结构的机械分解，从而将材料还原成浆料。 然后可以通过离心将其分离到其组分中，以便收获含有多能细胞的级分。 这些多能细胞可用于各种医疗过程以刺激愈合和组织再生。

公开(公告)号：US09090865B2

公开(公告)日：2015-07-28

申请号：US13284781

申请日：2011-10-28

Applicant: Dino Di Carlo ,  Soojung Hur

Inventor： Dino Di Carlo ,  Soojung Hur

IPC: C12M1/00 ,  G01N15/10 ,  B01L3/00

CPC classification number: G01N15/14 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/10 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/105 ,  G01N2015/1081 ,  G01N2015/1087 ,  G01N2015/1495

Abstract: A particle analysis system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet; and a particle analyzer configured to measure a size and a position of particles in the microchannel. A particle sorting system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; and a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet.

Abstract translation: 粒子分析系统包括入口; 惯性聚焦微通道，其设置在基底中并且在远端具有下游扩张区域，其中所述入口连接到所述微通道的近端; 在下游扩展区域连接到微通道的多个出口; 多个流体电阻器，其中每个流体电阻器连接到相应的出口; 以及粒子分析器，被配置为测量微通道中颗粒的尺寸和位置。 颗粒分选系统包括入口; 惯性聚焦微通道，其设置在基底中并且在远端具有下游扩张区域，其中所述入口连接到所述微通道的近端; 在下游扩展区域连接到微通道的多个出口; 和多个流体电阻器，其中每个流体电阻器连接到相应的出口。