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    Nucleic acid amplification with integrated multiplex dectection
    83.
    发明申请
    Nucleic acid amplification with integrated multiplex dectection 有权
    核酸扩增与综合多重检测

    公开(公告)号：US20060188896A1

    公开(公告)日：2006-08-24

    申请号：US11218838

    申请日：2005-09-02

    Applicant: Michael Seul ,  Nataliya Korzheva ,  Jiacheng Yang ,  Yi Zhang

    Inventor： Michael Seul ,  Nataliya Korzheva ,  Jiacheng Yang ,  Yi Zhang

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6865 ,  C12Q2563/149 ,  C12Q2563/107 ,  C12Q2537/143

    Abstract: A method mediated with in-vitro transcription (“IVT”) which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double stranded DNA: wherein, the selected strand forms the template for RNA synthesis. In one embodiment, double stranded DNA incorporating the T7 (or other) promoter sequence at the 3′ end or the 5′ end is produced, for example, by amplification of genomic DNA using the Polymerase Chain Reaction (PCR). Also disclosed are nested PCR designs permitting allele analysis in combination with strand selection by IVT. Further, in one embodiment of a homogeneous format for transcription-mediated amplification and multiplexed detection (which may be particularly suited for viral or pathogen detection), encoded microparticles display “looped” capture probe configurations permitting the generation of a signal upon capture of RNA product and real-time assay monitoring.

    Abstract translation: 描述了允许多元化DNA和RNA分析的小型化以及其中使用延长介导的多态性多重分析（eMAP））介导的体外转录（“IVT”）介导的方法作为分析步骤。 还描述了用IVT介导的用于从T7标记的双链DNA中选择指定链的方法：其中所选择的链形成用于RNA合成的模板。 在一个实施方案中，例如通过使用聚合酶链式反应（PCR）扩增基因组DNA来产生在3'末端或5'端掺入T7（或其它）启动子序列的双链DNA。 还公开了允许等位基因分析与IVT的链选择组合的巢式PCR设计。 此外，在用于转录介导的扩增和多重检测（其可能特别适用于病毒或病原体检测）的均匀形式的一个实施方案中，编码的微粒显示允许在捕获RNA产物时产生信号的“环状”捕获探针构型 和实时检测监测。

    Method of characterizing heterogeneity in receptor-ligand interactions and related method of assay signal histogram transformation
    84.
    发明申请
    Method of characterizing heterogeneity in receptor-ligand interactions and related method of assay signal histogram transformation 审中-公开
    表征受体 - 配体相互作用异质性的方法和相关方法的测定信号直方图转换

    公开(公告)号：US20060105390A1

    公开(公告)日：2006-05-18

    申请号：US10976686

    申请日：2004-10-29

    Applicant: Yi Zhang ,  Michael Seul

    Inventor： Yi Zhang ,  Michael Seul

    IPC: G01N33/53 ,  G06F19/00

    CPC classification number: G01N33/543

    Abstract: Disclosed is a method for increasing the degree of confidence in receptor-ligand and probe-target interaction assays involving ligand or target capture by a heterogeneous population of receptors or probes displayed on solid phase carriers. The method comprises a process of establishing a weight function reflecting the heterogeneity in the receptor population. The weight function is applied to “filter” the assay signal distribution (“histogram”) produced in an assay involving the interaction of immobilized receptors with ligands in an actual clinical sample in order to “sharpen” the assay signal intensity distribution.

    Abstract translation: 公开了一种用于增加受体配体的置信度的方法和涉及通过显示在固相载体上的异质群体的受体或探针进行的配体或靶捕获的探针 - 靶相互作用测定。 该方法包括建立反映受体群体异质性的权重函数的过程。 权重函数用于“过滤”在涉及固定化受体与实际临床样品中的配体的相互作用的测定中产生的测定信号分布（“直方图”），以“锐化”测定信号强度分布。

    On-chip analysis of particles and fractionation of particle mixtures using light-controlled electrokinetic assembly of particles near surfaces
    87.
    发明授权
    On-chip analysis of particles and fractionation of particle mixtures using light-controlled electrokinetic assembly of particles near surfaces 有权
    颗粒的片上分析和使用表面附近的颗粒的光控电动组装的颗粒混合物的分级

    公开(公告)号：US06706163B2

    公开(公告)日：2004-03-16

    申请号：US09813571

    申请日：2001-03-21

    Applicant: Michael Seul ,  Sukanta Banerjee ,  Kairali Podual

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Kairali Podual

    IPC: B01D5702

    CPC classification number: G01N15/0266 ,  B03C5/026 ,  C02F1/469 ,  G01N2015/0294

    Abstract: A method and apparatus for fractionation of a mixture of particles and for particle analysis are provided, in which LEAPS (“Light-controlled Electrokinetic Assembly of Particles near Surfaces”) is used to fractionate and analyze a plurality of particles suspended in an interface between an electrode and an electrolyte solution. A mixture of particles are fractionated according to their relaxation frequencies, which in turn reflect differences in size or surface composition of the particles. Particles may also be analyzed to determine their physical and chemical properties based on particle relaxation frequency and maximal velocity.

    Abstract translation: 提供了一种用于分级颗粒混合物和用于颗粒分析的方法和装置，其中使用LEAPS（“靠近表面的颗粒的光控电动组装”）分级分析悬浮在 电极和电解质溶液。 颗粒的混合物根据它们的松弛频率进行分级，这又反映了颗粒的尺寸或表面组成的差异。 还可以分析颗粒以基于颗粒松弛频率和最大速度确定其物理和化学性质。

    Un-supported polymeric film with embedded microbeads
    89.
    发明授权
    Un-supported polymeric film with embedded microbeads 有权
    具有嵌入微珠的未负载的聚合物膜

    公开(公告)号：US09436088B2

    公开(公告)日：2016-09-06

    申请号：US11761789

    申请日：2007-06-12

    Applicant: Michael Seul ,  Sukanta Banerjee ,  Kairali Podual ,  Ye Hong

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Kairali Podual ,  Ye Hong

    IPC: G01N33/553 ,  G03F7/16 ,  G03F7/004

    CPC classification number: C08J3/075 ,  C08J3/28 ,  C08J2333/26 ,  C08J2425/06 ,  C12Q1/6837 ,  G01N33/5005 ,  G01N33/545 ,  G03F7/0047 ,  G03F7/164 ,  Y10T428/249952 ,  Y10T428/25

    Abstract: The present invention relates to polymer-bead composites having a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix. The composite may be unattached to a solid support. The encoded beads have different biomolecules attached to their surfaces, and the encoding permits distinguishing beads having different biomolecules attached thereto. The present invention also relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites, including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films.

    Abstract translation: 本发明涉及具有嵌入亲水性聚合物基质中的编码珠粒的单层平面结晶组合物的聚合物 - 珠复合物。 复合材料可能不附着在固体支撑物上。 编码的珠粒具有连接到其表面的不同生物分子，并且编码允许区分附着有不同生物分子的珠粒。 本发明还涉及一种用于创建聚合物 - 微粒复合材料的功能组织的空间图案化组件的系统方法，包括图案化的自支撑有机（聚合物）膜和有机 - 聚合物 - 微粒的AC电场介导装配 定制组合和形态的复合材料。 本发明还涉及这种功能组件在材料科学和生物学中的应用。 其他应用领域包括传感器，催化剂，膜和微反应器以及用于产生多功能薄膜的小型化形式。

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