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公开(公告)号：US06238624B1
公开(公告)日：2001-05-29
申请号：US08726278
申请日：1996-10-04
IPC分类号： G01N1506
CPC分类号： C07K1/04 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00822 , B01J2219/00828 , B01J2219/00844 , B01J2219/00853 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/0877 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/045 , C07K1/047 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L29/7834 , H01L2224/45144 , H01L2224/95144 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/0102 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , Y10S435/808 , Y10S435/814 , Y10S436/805 , Y10T436/25125 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该设备可以电子控制特定绑定实体到特定微位置的传输和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
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2.发明授权
公开(公告)号：US6051380A
公开(公告)日：2000-04-18
申请号：US986065
申请日：1997-12-05
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
IPC分类号： C12N15/09 , B01J19/00 , B01L3/00 , C07B61/00 , C07H21/00 , C07K1/04 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/02 , G11C19/00 , H01L21/336 , H01L21/98 , H01L29/78
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B82Y10/00 , B82Y5/00 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/04 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该装置可以电子控制特定结合实体到特定微位置的转运和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在所寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
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公开(公告)号：US6048690A
公开(公告)日：2000-04-11
申请号：US855058
申请日：1997-05-14
IPC分类号： B01J19/00 , B01L3/00 , B82B3/00 , C07B61/00 , C07H21/00 , C07K1/04 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N33/543 , G02B6/122 , G06N3/00 , G06N3/06 , G06N3/12 , G11B7/0037 , G11B7/0045 , G11B7/005 , G11B7/24 , G11B7/244 , G11C13/02 , G11C13/04 , G11C19/00 , H01L21/336 , H01L21/98 , H01L29/78 , H01L51/00 , H01L51/30
CPC分类号： B01J19/0046 , B01J19/0093 , B01L3/502761 , B01L3/5085 , B82B3/00 , B82Y10/00 , B82Y20/00 , B82Y30/00 , B82Y40/00 , B82Y5/00 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6818 , C12Q1/6837 , G01N33/54366 , G02B6/1225 , G06N3/002 , G06N3/061 , G06N3/123 , G11B7/0037 , G11B7/0045 , G11B7/00455 , G11B7/005 , G11B7/0052 , G11B7/24 , G11B7/244 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L24/95 , H01L25/50 , H01L51/0595 , B01J2219/00315 , B01J2219/00317 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00545 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00707 , B01J2219/00711 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L2200/025 , B01L2200/0663 , B01L2200/12 , B01L2300/0636 , B01L2300/0645 , B01L2400/0421 , C07B2200/11 , C12Q1/683 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , H01L2924/01005 , H01L2924/01006 , H01L2924/01011 , H01L2924/01012 , H01L2924/01013 , H01L2924/01019 , H01L2924/01033 , H01L2924/01039 , H01L2924/01047 , H01L2924/01057 , H01L2924/01061 , H01L2924/01063 , H01L2924/01065 , H01L2924/01074 , H01L2924/01075 , H01L2924/01078 , H01L2924/01082 , H01L2924/12042 , H01L51/0093
摘要： Methods for electronic perturbation of fluorescence, chemilluminescence and other emissive materials provide for molecular biological analysis. In a preferred method for hybridization analysis of a sample, an electronic stringency control device is used to perform the steps of: providing the sample, a first probe with a fluorescent label and a second probe with a label under hybridization conditions on the electronic stringency control device, forming a hybridization product, subjecting the hybridization product to an electric field force, monitoring the fluorescence from the hybridization product, and analyzing the fluorescent signal. The label preferably serves as a quencher for the fluorescent label. In yet another aspect of this invention, a method for achieving electronic fluorescence perturbation on an electronic stringency control device comprising the steps of: locating a first polynucleotide and a second polynucleotide adjacent the electronic stringency control device, the first polynucleotide and second polynucleotide being complementary over at least a portion of their lengths and forming a hybridization product, the hybridization product having an associated environmental sensitive emission label, subjecting the hybridization product and label to a varying electrophoretic force, monitoring the emission from the label, and analyzing the monitored emission to determine the electronic fluorescence perturbation effect. In yet another aspect of this invention, a method is provided for electronic perturbation catalysis of substrate molecules on an electronic control device containing at least one microlocation comprising the steps of: immobilizing on the microlocation an arrangement of one or more reactive groups, exposing the reactive groups to a solution containing the substrate molecules of interest, and applying an electronic pulsing sequence which causes charge separation between the reactive groups to produce a catalytic reaction on the substrate molecules.
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4.发明授权Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics 有权用于分子生物学分析和诊断的自寻址自组装微电子系统和器件公开(公告)号：US07947486B2
公开(公告)日：2011-05-24
申请号：US11702353
申请日：2007-02-05
CPC分类号： C07K1/04 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00822 , B01J2219/00828 , B01J2219/00844 , B01J2219/00853 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/0877 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/045 , C07K1/047 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L29/7834 , H01L2224/45144 , H01L2224/95144 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/0102 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , Y10S435/808 , Y10S435/814 , Y10S436/805 , Y10T436/25125 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.
摘要翻译： 描述了用于分析从平台上的细胞样品获得的核酸的方法。 提供了具有细胞选择剂，核酸选择剂和微位点阵列的平台，其中至少一个微位置具有相关联的捕获序列。 细胞选择器与细胞样品接触，其中一部分细胞保持与细胞选择器相关联。 将与细胞选择器相关联的至少一部分细胞裂解释放核酸样品。 然后使核酸选择剂与核酸样品接触，使得核酸样品的一部分保留与核酸选择器相关联。 然后将相关核酸样品从核酸选择器中释放，然后与微位点阵列接触，使得至少一部分释放的核酸样品与捕获序列杂交。
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公开(公告)号：US06753148B2
公开(公告)日：2004-06-22
申请号：US10108280
申请日：2002-03-25
申请人： Ronald G. Sosnowski , Eugene Tu
发明人： Ronald G. Sosnowski , Eugene Tu
IPC分类号： C12Q168
CPC分类号： H01L29/6656 , B01J2219/00653 , B01J2219/00659 , C12Q1/6825 , C12Q1/6827 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C12Q2565/518 , C12Q2537/125 , C12Q2525/151 , C12Q2565/607
摘要： Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0). Concordance and discordance among the hybridization complex assays at the test sites is determined at least in part by hybridization stability. Electronic stringency control may be utilized. Applications include paternity testing, forensic use, and disease diagnostics, such as for the identification of the existence of a clonal tumor.
摘要翻译： 提供了方法和装置，用于分析和确定基因靶标中重复单元的性质。 在本发明的一种方法中，遗传靶点中重复单元的性质通过提供排列在多个测试位点上的多个杂交复合物测定的步骤确定，其中杂交复合物测定法至少包括核酸靶 含有简单的重复DNA序列，具有第一独特侧翼序列和n个重复单元的捕获探针，其中n = 0,1,2。 。 。 或其部分与靶序列互补，以及具有与相同靶序列链互补的所选序列的报道探针，其中所述报告基因的选定序列包含第二独特侧翼序列和m个重复单元，其中m = 0， 1,2。 。 。 或其级分，但是其中捕获探针加上报道探针中的重复单元的总和大于0（n + m> 0）。 至少部分通过杂交稳定性测定在测试位点的杂交复合物测定中的一致性和不一致性。 可以使用电子严格控制。 应用包括亲子鉴定，法医使用和疾病诊断，例如鉴定克隆性肿瘤的存在。
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6.发明授权公开(公告)号：US06518022B1
公开(公告)日：2003-02-11
申请号：US09444539
申请日：1999-11-22
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
IPC分类号： C12Q168
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该装置可以电子控制特定结合实体到特定微位置的转运和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在所寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
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7.发明授权公开(公告)号：US08114589B2
公开(公告)日：2012-02-14
申请号：US11726520
申请日：2007-03-22
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.
摘要翻译： 描述了用于电子稳定在微电子器件的测试位点处结合的核酸杂交的方法。 提供第一和第二带负电荷的核酸，第二核酸与测试部位结合。 将具有小于100mS / cm 2的电导的两性离子缓冲液施加到微电子器件。 将电流施加到测试部位以使测试部位正偏置，使得将第一带负电荷的核酸转运到具有结合第二带负电荷核酸的正偏压测试位点。 在测试位点，第一和第二带负电荷的核酸杂交。 两性离子缓冲液在电流影响下获得净正电荷，使得带正电的两性离子缓冲液通过降低第一和第二带负电的核酸之间的排斥来稳定杂交。
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8.发明申请Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics 有权用于分子生物学分析和诊断的自寻址自组装微电子系统和器件公开(公告)号：US20100173792A1
公开(公告)日：2010-07-08
申请号：US11702353
申请日：2007-02-05
IPC分类号： C40B30/04
CPC分类号： C07K1/04 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00822 , B01J2219/00828 , B01J2219/00844 , B01J2219/00853 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/0877 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/045 , C07K1/047 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L29/7834 , H01L2224/45144 , H01L2224/95144 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/0102 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , Y10S435/808 , Y10S435/814 , Y10S436/805 , Y10T436/25125 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.
摘要翻译： 描述了用于分析从平台上的细胞样品获得的核酸的方法。 提供了具有细胞选择剂，核酸选择剂和微位点阵列的平台，其中至少一个微位置具有相关联的捕获序列。 细胞选择器与细胞样品接触，其中一部分细胞保持与细胞选择器相关联。 将与细胞选择器相关联的至少一部分细胞裂解释放核酸样品。 然后使核酸选择剂与核酸样品接触，使得核酸样品的一部分保留与核酸选择器相关联。 然后将相关核酸样品从核酸选择器中释放，然后与微位点阵列接触，使得至少一部分释放的核酸样品与捕获序列杂交。
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公开(公告)号：US07172864B1
公开(公告)日：2007-02-06
申请号：US09490965
申请日：2000-01-24
CPC分类号： B01L3/5085 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502707 , B01L2200/0647 , B01L2300/0636 , B01L2300/0645 , B01L2300/0877 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L2224/45144 , H01L2224/95145 , H01L2924/10253 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该设备可以电子控制特定绑定实体到特定微位置的传输和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
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公开(公告)号：US06395493B1
公开(公告)日：2002-05-28
申请号：US09645757
申请日：2000-08-24
申请人： Ronald G. Sosnowski , Eugene Tu
发明人： Ronald G. Sosnowski , Eugene Tu
IPC分类号： C12Q168
CPC分类号： H01L29/6656 , B01J2219/00653 , B01J2219/00659 , C12Q1/6825 , C12Q1/6827 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C12Q2565/518 , C12Q2537/125 , C12Q2525/151 , C12Q2565/607
摘要： Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0). Concordance and discordance among the hybridization complex assays at the test sites is determined at least in part by hybridization stability. Electronic stringency control may be utilized. Applications include paternity testing, forensic use, and disease diagnostics, such as for the identification of the existence of a clonal tumor.
摘要翻译： 提供了方法和装置，用于分析和确定基因靶标中重复单元的性质。 在本发明的一种方法中，遗传靶点中重复单元的性质通过提供排列在多个测试位点上的多个杂交复合物测定的步骤确定，其中杂交复合物测定法至少包括核酸靶 含有简单的重复DNA序列，具有第一独特侧翼序列和n个重复单元的捕获探针，其中n = 0,1,2。 。 。 或其部分与靶序列互补，以及具有与相同靶序列链互补的所选序列的报道探针，其中所述报告基因的选定序列包含第二独特侧翼序列和m个重复单元，其中m = 0， 1,2。 。 。 或其级分，但是其中捕获探针加上报道探针中的重复单元的总和大于0（n + m> 0）。 至少部分通过杂交稳定性测定在测试位点的杂交复合物测定中的一致性和不一致性。 可以使用电子严格控制。 应用包括亲子鉴定，法医使用和疾病诊断，例如鉴定克隆性肿瘤的存在。
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