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    Plating process and structure
    95.
    发明授权
    Plating process and structure 有权
    电镀工艺和结构

    公开(公告)号：US08759118B2

    公开(公告)日：2014-06-24

    申请号：US13297845

    申请日：2011-11-16

    Applicant: Chin-Fu Kao ,  Cheng-Lin Huang ,  Jing-Cheng Lin

    Inventor： Chin-Fu Kao ,  Cheng-Lin Huang ,  Jing-Cheng Lin

    IPC: H01L21/66 ,  H01L23/58

    CPC classification number: H01L22/32

    Abstract: A system and method for plating a contact is provided. An embodiment comprises forming protective layers over a contact and a test pad, and then selectively removing the protective layer over the contact without removing the protective layer over the test pad. With the protective layer still on the test pad, a conductive layer may be plated onto the contact without plating it onto the test pad. After the contact has been plated, the protective layer over the contact may be removed.

    Abstract translation: 提供了一种用于电镀触点的系统和方法。 一个实施例包括在触点和测试垫上形成保护层，然后在触头上选择性地去除保护层，而不需要在测试垫上移除保护层。 在保护层仍在测试焊盘上的情况下，可以将导电层电镀到触点上，而不将其覆盖在测试焊盘上。 接触电镀后，触点上的保护层可以被去除。

    Asymmetric PCR amplification, its special primer and application
    96.
    发明授权
    Asymmetric PCR amplification, its special primer and application 有权
    不对称PCR扩增，其特殊引物及应用

    公开(公告)号：US08735067B2

    公开(公告)日：2014-05-27

    申请号：US11661069

    申请日：2004-11-22

    Applicant: Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    Inventor： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

    Abstract: The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

    Abstract translation: 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。

    Controlling defects in thin wafer handling
    97.
    发明授权
    Controlling defects in thin wafer handling 有权
    控制薄晶片处理中的缺陷

    公开(公告)号：US08722540B2

    公开(公告)日：2014-05-13

    申请号：US12841874

    申请日：2010-07-22

    Applicant: Yu-Liang Lin ,  Weng-Jin Wu ,  Jing-Cheng Lin

    Inventor： Yu-Liang Lin ,  Weng-Jin Wu ,  Jing-Cheng Lin

    IPC: H01L21/302 ,  H01L21/461

    CPC classification number: H01L21/6835 ,  H01L2221/68327 ,  H01L2221/6834

    Abstract: A method includes bonding a wafer on a carrier through an adhesive, and performing a thinning process on the wafer. After the step of performing the thinning process, a portion of the adhesive not covered by the wafer is removed, while the portion of the adhesive covered by the wafer is not removed.

    Abstract translation: 一种方法包括通过粘合剂将晶片接合在载体上，并在晶片上进行稀化处理。 在进行稀化处理的步骤之后，去除未被晶片覆盖的粘合剂的一部分，同时由晶片覆盖的粘合剂部分未被除去。

    Methods for assaying analytes using photoelectrochemical labels
    99.
    发明授权
    Methods for assaying analytes using photoelectrochemical labels 有权
    使用光电化学标记测定分析物的方法

    公开(公告)号：US08603750B2

    公开(公告)日：2013-12-10

    申请号：US10535905

    申请日：2003-05-06

    Applicant: Lianghong Guo ,  Dong Dong ,  Dong Zheng ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

    Inventor： Lianghong Guo ,  Dong Dong ,  Dong Zheng ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

    IPC: G01N33/53 ,  G01N21/76

    CPC classification number: G01N33/582 ,  C12Q1/6816 ,  G01N2458/30 ,  C12Q2563/113 ,  C12Q2523/313

    Abstract: The present invention provides apparatus, kits and methods for assaying analytes using photoelectrochemical molecules as labels. Specifically, the present invention provides a method for assaying an analyte, comprising: a) contacting a sample suspected of containing an analyte with a reactant capable of binding and/or reacting with the analyte under suitable conditions to allow binding of analyte, if present in the sample, to the reactant; and b) assessing binding and/or reacting between the analyte and the reactant to determine presence and/or amount of analyte in the sample, wherein the reactant, analyte, or additional reactant or additional analyte or analyte analog is labeled with a photoelectrochemically active molecule. The assessing step also comprises converting the photoelectrochemically active molecule with light to an excited state in the presence of an electrode, and assessing an electric current generated by an electron transfer between the excited photoelectrochemically active molecule and the electrode.

    Abstract translation: 本发明提供了使用光电化学分子作为标记来测定分析物的装置，试剂盒和方法。 具体地，本发明提供了一种用于分析分析物的方法，其包括：a）在合适的条件下将疑似含有分析物的样品与能够与分析物结合和/或反应的反应物接触，以使分析物（如果存在于 样品，反应物; 和b）评估分析物和反应物之间的结合和/或反应以确定样品中分析物的存在和/或量，其中反应物，分析物或附加反应物或其他分析物或分析物类似物用光电化学活性分子标记 。 评估步骤还包括在电极存在下将光电化学活性分子与光转化为激发态，并评估由激发的光电化学活性分子和电极之间的电子转移产生的电流。

    Microvalve controlled precision fluid dispensing apparatus with a self-purging feature and method for use
    100.
    发明授权
    Microvalve controlled precision fluid dispensing apparatus with a self-purging feature and method for use 有权
    具有自动清洗功能和使用方法的微型阀控精密流体分配装置

    公开(公告)号：US08597592B2

    公开(公告)日：2013-12-03

    申请号：US11991723

    申请日：2006-01-13

    Applicant: Dong Wang ,  Kun Zou ,  Jing Cheng

    Inventor： Dong Wang ,  Kun Zou ,  Jing Cheng

    IPC: B01L3/00

    CPC classification number: B41J2/17596 ,  B01J2219/0036 ,  B01J2219/00364 ,  B01L3/0265 ,  B01L2400/0478 ,  B01L2400/0622 ,  B41J2/175 ,  G01N2035/00247 ,  G01N2035/1041 ,  Y10T436/2575

    Abstract: An apparatus and methods for the precise, repeatable dispensing of small, sample fluid volumes, especially as related to the printing of microarrays for biological and/or chemical testing. A pressure tuning module meters fluid volumes for aspiration and dispensation and builds pressure for fluid dispensation, in conjunction with a microvalve that precisely controls the volume of the fluid dispensed under the built pressure. A pressure source can be switched in line to efficiently purge the apparatus of residual sample fluid. A working fluid can be optionally aspirated into the system, prior to aspirating the sample fluid, in order to maximize sample fluid recovery.

    Abstract translation: 用于精确，可重复分配小的样品流体体积的装置和方法，特别是与用于生物和/或化学测试的微阵列的印刷相关。 压力调节模块计量用于抽吸和分配的流体体积，并建立用于流体分配的压力，以及精确控制在内置压力下分配的流体的体积的微型阀。 压力源可以排成一行，以有效地吹扫残留样品液的装置。 在抽吸样品流体之前，可以将工作流体任选地吸入系统，以使样品液体回收最大化。

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