公开(公告)号：US20170362591A1

公开(公告)日：2017-12-21

申请号：US15539617

申请日：2015-12-24

Applicant: National Institutes of Biomedical Innovation, Health and Nutrition ,  GeneDesign, Inc.

Inventor： Ken Ishii ,  Taiki Aoshi ,  Hideaki Sato

IPC: C12N15/117 ,  A61K9/127

CPC classification number: C12N15/117 ,  A61K9/127 ,  A61K31/7088 ,  C12N15/09 ,  C12N2310/17 ,  C12N2310/315

Abstract: Provided are non-aggregating immunostimulatory oligonucleotides. The present invention also provides a delivery agent for an immunostimulatory-oligonucleotide nucleic acid medicine, said delivery agent including a nucleic acid that contains a phosphorothioated nucleotide. According to another aspect, the present invention further provides an oligonucleotide including a bioactive core, and a nucleic acid that contains a phosphorothioated nucleotide. The present invention yet further provides an immunostimulator including the bioactive core of a type A/D, type B/K, or type C immunostimulatory oligonucleotide.

公开(公告)号：US20170362589A1

公开(公告)日：2017-12-21

申请号：US15603013

申请日：2017-05-23

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: C12N15/10 ,  C12N15/74 ,  C40B50/18 ,  C12N15/66 ,  B01J19/00 ,  C12N15/09

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein

公开(公告)号：US20170356033A1

公开(公告)日：2017-12-14

申请号：US15524656

申请日：2015-11-06

Applicant: Osaka City University

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6851 ,  C12Q1/6883 ,  G01N33/574

Abstract: A method in which a mutant gene present in a gene pool mixedly with a large number of wild-type genes can be simply, inexpensively and sensitively detected is developed and provided. A clamping probe that is connected to a target nucleic acid molecule in two regions of first and second target nucleic acid complementary regions so that a wild-type target nucleic acid molecule and a mutant-type target nucleic acid molecule can be distinguished from each other depending on a difference in complementarity to these target nucleic acid molecules is provided.

公开(公告)号：US09839894B2

公开(公告)日：2017-12-12

申请号：US15233835

申请日：2016-08-10

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: C12N15/10 ,  B01J19/00 ,  C40B40/06 ,  C12N15/66 ,  C40B50/00 ,  C40B50/18 ,  C40B50/14

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20170349917A1

公开(公告)日：2017-12-07

申请号：US15532712

申请日：2014-12-05

Applicant: HONDA MOTOR CO., LTD.

Inventor： Yoshiki Tsuchida ,  Ikumi Kurihara ,  Tomohiro Imai ,  Iku Koike

IPC: C12P7/06 ,  C12R1/72 ,  C12N9/10 ,  C12N9/04 ,  C12N15/81

CPC classification number: C12P7/06 ,  C12N9/0006 ,  C12N9/1022 ,  C12N15/09 ,  C12N15/815 ,  C12R1/72 ,  C12Y101/01001 ,  C12Y202/01002

Abstract: An object of the present invention is to obtain a fermentative yeast having a highly efficient ethanol production without introducing a foreign gene. A further object is to obtain a fermentative yeast that is resistant to proliferation inhibitors such as organic acids, which prevent the growth of the fermentative yeast. Yeast having improved ethanol production ability was generated by introducing transaldolase and alcohol dehydrogenase gene by self-cloning to Meyerozyma guilliermondii that can produce ethanol effectively from pentose and hexose obtained by breeding. This fermentative yeast is deposited to NITE Patent Microorganisms Depositary under the accession number NITE ABP-01976.

公开(公告)号：US20170327798A1

公开(公告)日：2017-11-16

申请号：US15522798

申请日：2015-10-28

Applicant: Amano Enzyme Inc.

Inventor： Kazunori Yoshida ,  Kyoichi Nishio

IPC: C12N9/04 ,  C12Q1/60 ,  C12Q1/32 ,  C12Q1/26

CPC classification number: C12N9/0006 ,  C12N15/09 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/60 ,  C12Y101/01 ,  C12Y101/03006 ,  G01N2333/904

Abstract: The object is to provide a novel enzyme exhibiting cholesterol dehydrogenase activity. Provided is a mutant enzyme having an amino acid sequence of a microorganism-derived cholesterol oxidase, which is composed of: (1) an amino acid corresponding to the amino acid at the position 113 of the amino acid sequence of SEQ ID NO: 1; (2) an amino acid corresponding to the amino acid at the position 362 of the amino acid sequence of SEQ ID NO: 1; (3) an amino acid corresponding to the amino acid at the position 402 of the amino acid sequence of SEQ ID NO: 1; (4) an amino acid corresponding to the amino acid at the position 412 of the amino acid sequence of SEQ ID NO: 1; (5) an amino acid corresponding to the amino acid at the position 468 of the amino acid sequence of SEQ ID NO: 1; and others.

公开(公告)号：US20170327600A1

公开(公告)日：2017-11-16

申请号：US15324446

申请日：2015-07-08

Applicant: KYOTO UNIVERSITY

Inventor： Hirohide Saito ,  Eriko Osada

IPC: C07K19/00 ,  C07K16/32 ,  C12N15/113

CPC classification number: C07K19/00 ,  C07K16/32 ,  C07K2318/20 ,  C07K2319/00 ,  C12N15/09 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/3513

Abstract: An RNA and protein delivery system utilizing an RNA-protein complex having a higher-order structure, and an RNA-protein complex having a novel higher-order structure.

公开(公告)号：US20170321235A1

公开(公告)日：2017-11-09

申请号：US15533215

申请日：2015-12-25

Applicant: KIKKOMAN CORPORATION

Inventor： Seiichi HARA ,  Keiko KUROSAWA ,  Keiichi ICHIKAWA

IPC: C12P13/04 ,  C12N9/10

CPC classification number: C12P13/04 ,  C12N9/10 ,  C12N9/1007 ,  C12N9/13 ,  C12N15/09 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69 ,  C12Y201/01 ,  C12Y208/01007

Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

公开(公告)号：US20170306296A1

公开(公告)日：2017-10-26

申请号：US15516107

申请日：2015-10-02

Applicant: JICHI MEDICAL UNIVERSITY

Inventor： Hitoshi ENDO ,  Yasumitsu NAGAO ,  Yutaka HANAZONO ,  Kaoru TOMINAGA ,  Tsukasa OHMORI

IPC: C12N5/074 ,  C12Q1/02 ,  C12N5/0735 ,  C12N15/09 ,  C12Q1/68 ,  C12N5/00

CPC classification number: C12N5/0696 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2267/02 ,  A01K2267/03 ,  C12N5/00 ,  C12N5/0604 ,  C12N5/0606 ,  C12N15/09 ,  C12N2501/00 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/5073

Abstract: The present application relates to a method for reestablishing stem cells capable of forming chimeras, and cells obtained by the method. The method of the present invention is a technique for monocloning stem cells, for example, capable of forming chimeras from a heterogeneous cell population to obtain high-quality stem cells.

公开(公告)号：US20170305974A1

公开(公告)日：2017-10-26

申请号：US15502524

申请日：2015-05-22

Applicant: IDEMITSU KOSAN CO., LTD.

Inventor： Kazutoshi SAWADA ,  Takeshi MATSUI ,  Kazuyoshi KOIKE

IPC: C07K14/005 ,  A61K39/385 ,  A61K39/12 ,  C12N15/82 ,  C07K14/705 ,  C12N7/00 ,  A61K39/00

CPC classification number: C07K14/005 ,  A01H5/00 ,  A61K39/12 ,  A61K39/385 ,  A61K2039/542 ,  A61K2039/543 ,  A61K2039/552 ,  A61K2039/6031 ,  C07K14/08 ,  C07K14/245 ,  C07K14/70575 ,  C07K19/00 ,  C07K2319/40 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/8258 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10071 ,  C12P21/02

Abstract: An object of the present invention is to optimize, and to increase the accumulation amount of, GP5 antigen, in order to enhance the performance of a PRRS vaccine. The present invention provides a fusion protein comprising an ectodomain (ectGP5) of Glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome (PRRS) virus, and an adjuvant protein.