公开(公告)号：US20180243741A1

公开(公告)日：2018-08-30

申请号：US15829665

申请日：2017-12-01

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Lincoln McBride ,  Geoffrey Facer

IPC: B01L3/00 ,  C12Q1/686 ,  G01N27/453 ,  B01L7/00

CPC classification number: B01L3/502738 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  G01N27/44791 ,  G01N27/453 ,  C12Q2561/113 ,  C12Q2563/159 ,  C12Q2531/113

Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.

公开(公告)号：US09952126B2

公开(公告)日：2018-04-24

申请号：US13781313

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C Norris ,  Dominique Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC: C12P19/34 ,  G01N1/28 ,  C12Q1/68 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20170175170A1

公开(公告)日：2017-06-22

申请号：US15382360

申请日：2016-12-16

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/68 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20170096713A1

公开(公告)日：2017-04-06

申请号：US15266787

申请日：2016-09-15

Applicant: California Institute of Technology ,  Fluidigm Corporation

Inventor： Stanley N. Lapidus ,  Stephen R. Quake

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6869 ,  C12Q2600/156

Abstract: The invention provides methods for determining the presence of a disease by comparing a sequence from a single target molecule with a predetermined sequence that is associated with a specific disease.

公开(公告)号：US09593698B2

公开(公告)日：2017-03-14

申请号：US14072243

申请日：2013-11-05

Applicant: Fluidigm Corporation

Inventor： David Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

IPC: F16C3/00 ,  F16K99/00 ,  B01L3/00 ,  F15C3/00 ,  F15C3/04 ,  G06F17/50 ,  H01H29/00

CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

Abstract: A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.

Abstract translation: 微制造的流体单向阀包括具有流通通道的微加工弹性体材料。 微制造的流体单向阀还包括在流动通道中附接到弹性体材料的弹性体翼片。 弹性翼片在流通通道中形成密封，以防止流体沿第一方向流过通过通道并允许流体沿第二方向流过通过通道的流体。

公开(公告)号：US20160362741A1

公开(公告)日：2016-12-15

申请号：US15013915

申请日：2016-02-02

Applicant: Fluidigm Corporation

Inventor： Stanley N. Lapidus

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2563/107 ,  C12Q2533/101

Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。

公开(公告)号：US09498776B2

公开(公告)日：2016-11-22

申请号：US14720305

申请日：2015-05-22

Applicant: Fluidigm Corporation

Inventor： David Cohen ,  Andrew May ,  Martin Pieprzyk ,  Brian Fowler ,  Kim Huat Lee ,  Jun Yan ,  Ming Fang Zhou ,  Seng Beng Ng

IPC: B01L99/00 ,  B01L3/00 ,  B01L9/00 ,  B01L7/00 ,  G01N1/40

CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

公开(公告)号：US20160251714A1

公开(公告)日：2016-09-01

申请号：US15055252

申请日：2016-02-26

Applicant: Fluidigm Corporation

Inventor： Carolyn G. Conant ,  Tze Howe Charn ,  Jason A. A. West ,  Xiaohui Wang

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0609 ,  B01L2300/0893 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

Abstract translation: 本文描述的是基于细胞的分析方法，包括将核酸序列并入来自细胞群体的反应产物中的方法，其中所述核酸序列单独地或以小组细胞单独引入每个细胞的反应产物中。 本文还描述了允许至少两种试剂分别递送至每个细胞或细胞组的基质型微流体装置，以及在该方法和装置中有用的引物组合。

公开(公告)号：US20160238623A1

公开(公告)日：2016-08-18

申请号：US15136610

申请日：2016-04-22

Applicant: Fluidigm Corporation

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: G01N35/00 ,  G01N21/64 ,  C12Q1/68 ,  B01L3/00 ,  G01N35/10

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: A system includes a platform including one or more workstations and a microfluidic input device coupled to the one or more workstations. The microfluidic input device is adapted to receive a microfluidic device from a user. The system also includes a robotic device comprising a robotic arm and disposed on the platform. The robotic arm is capable of accessing the one or more workstations and is configured to transfer a plurality of sample solutions from a first spatial location to the microfluidic device when coupled to the microfluidic input device. The system further includes a multi-pixel image capturing device optically coupled to the microfluidic device and an image processing device operably coupled to the multi-pixel image capturing device. The multi-pixel image capturing device is adapted to capture a plurality of multi-pixel images. The image processing device is configured to receive the plurality of multi-pixel images.

Abstract translation: 系统包括包括一个或多个工作站的平台和耦合到该一个或多个工作站的微流体输入设备。 微流体输入装置适于从用户接收微流体装置。 该系统还包括机器人装置，其包括机器臂并且设置在平台上。 机器臂能够访问一个或多个工作站，并且被配置为当耦合到微流体输入装置时将多个样品溶液从第一空间位置传送到微流体装置。 该系统还包括光学耦合到微流体装置的多像素图像捕获装置和可操作地耦合到多像素图像捕获装置的图像处理装置。 多像素图像捕获装置适于捕获多个多像素图像。 图像处理装置被配置为接收多个多像素图像。

公开(公告)号：US20160208322A1

公开(公告)日：2016-07-21

申请号：US14723872

申请日：2015-05-28

Applicant: Fluidigm Corporation

Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.