公开(公告)号：US09702822B2

公开(公告)日：2017-07-11

申请号：US13973940

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Austin P. So ,  Duc Do

IPC: C12Q1/68 ,  C12P19/34 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2525/204 ,  C12Q2563/159

Abstract: Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

公开(公告)号：US20170191997A1

公开(公告)日：2017-07-06

申请号：US15388778

申请日：2016-12-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton T. McKee ,  William Strong ,  Mingde Zhu ,  Emmanuel Quiroz

IPC: G01N33/558 ,  G01N33/543

CPC classification number: G01N33/558 ,  G01N33/54386

Abstract: A lateral flow apparatus is provided.

公开(公告)号：US20170189908A1

公开(公告)日：2017-07-06

申请号：US15395199

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Dzenitis ,  Stefano Schiaffino

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/0241 ,  B01L3/545 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/021 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2400/049

Abstract: The present disclosure provides systems and methods for performing droplet assays with automatic calibration. An exemplary assay system may comprise a cartridge including a plurality of droplet generators to form emulsions of droplets having a same nominal volume. A tag may be associated with the cartridge and may encode calibration data or an identifier thereof. The calibration data may include a respective value specific to each droplet generator. The system further may include a detection system to detect a signal representing an analyte from droplets of each emulsion, and a reader to read the calibration data or the identifier from the tag. The system still further may include a processor configured to receive the signal and the calibration data and to calculate, for each emulsion, a concentration of an analyte using at least the signal and the respective value specific to the droplet generator that formed the emulsion.

公开(公告)号：US09688969B2

公开(公告)日：2017-06-27

申请号：US14845234

申请日：2015-09-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter B. Vander Horn ,  Yan Wang

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/00 ,  C12N9/1241 ,  C12Y207/07007

Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.

公开(公告)号：US09687848B2

公开(公告)日：2017-06-27

申请号：US15230757

申请日：2016-08-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Anthony J. Makarewicz, Jr. ,  Luc Bousse ,  Stefano Schiaffino

IPC: B01L3/00 ,  C12N15/10 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/04 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L2400/0677 ,  B01L2400/084 ,  C12N15/1075 ,  C12Q1/6806 ,  Y10T436/2575

Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

公开(公告)号：US09678923B2

公开(公告)日：2017-06-13

申请号：US13861143

申请日：2013-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Paul J Patt

IPC: G01F17/00 ,  G01N15/00 ,  G01N15/02 ,  G06F17/00 ,  B01L3/02 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

公开(公告)号：US20170160186A1

公开(公告)日：2017-06-08

申请号：US15376504

申请日：2016-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Daniel Nelson Fox ,  Nathan Michael Gaskill-Fox

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N1/28 ,  G01N1/286 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1409 ,  Y10T137/0324 ,  Y10T137/7287 ,  Y10T137/7303 ,  Y10T137/85978

Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.

公开(公告)号：US09671402B2

公开(公告)日：2017-06-06

申请号：US14634209

申请日：2015-02-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton McKee

IPC: G01N33/558

CPC classification number: G01N33/558 ,  G01N33/54366

Abstract: Methods, compositions, and kits for performing analyte detection in a lateral flow assay.

公开(公告)号：US09663766B2

公开(公告)日：2017-05-30

申请号：US14808699

申请日：2015-07-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Mark Irwin Fitchmun

IPC: C12N7/00 ,  B01D15/36

CPC classification number: C12N7/00 ,  B01D15/362 ,  B01D15/363 ,  C12N15/86 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10351

Abstract: Methods of purifying an adenovirus from an impure preparation are provided. In some embodiments, a combination of mixed mode chromatography and anion exchange chromatography is used to purify the adenovirus.

公开(公告)号：US20170144116A1

公开(公告)日：2017-05-25

申请号：US15365888

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Donald A. Masquelier

IPC: B01F3/08 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  B01F13/00 ,  C12Q1/68

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.