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公开(公告)号：US07759132B2
公开(公告)日：2010-07-20
申请号：US11585491
申请日：2006-10-23
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.
摘要翻译： 提供了执行微流体取样的方法。 该方法包括提供包括第一，第二和第三电极的布置的基板，其中第二电极介于第一和第三电极之间。 该方法还包括使流体输入连续地流动到第一电极并且将第一，第二和第三电极偏压到第一电压以使流体输入的一部分扩展穿过第二和第三电极。 该方法还包括将第二电极偏压到不同于第一电压的第二电压，以在第三电极上形成液滴，液滴与流体输入分离。
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公开(公告)号：US20100143963A1
公开(公告)日：2010-06-10
申请号：US12707081
申请日：2010-02-17
申请人： Michael G. Pollack , Philip Paik
发明人： Michael G. Pollack , Philip Paik
CPC分类号： B01L3/502792 , B01L2200/025 , B01L2200/04 , B01L2300/089 , B01L2400/0427 , G01N21/59 , G01N2021/035 , G01N2035/1046
摘要： The invention provides a droplet actuator drive. In certain embodiments, the droplet actuator drive may include a detection apparatus for sensing a property of a droplet on a droplet actuator; circuitry for controlling the detection apparatus electronically coupled to the detection apparatus; a droplet actuator cartridge connector; circuitry for controlling a droplet actuator coupled to the droplet actuator connector; and/or a means for coupling the droplet actuator circuitry to a processor. Systems, kits and methods of conducting assays are also provided.
摘要翻译： 本发明提供一种液滴致动器驱动装置。 在某些实施例中，液滴致动器驱动器可以包括用于感测液滴致动器上的液滴的特性的检测装置; 用于控制检测装置的电路，电子耦合到检测装置; 液滴致动器盒连接器; 用于控制耦合到液滴致动器连接器的液滴致动器的电路; 和/或用于将液滴致动器电路耦合到处理器的装置。 还提供了进行测定的系统，试剂盒和方法。
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公开(公告)号：US20100068764A1
公开(公告)日：2010-03-18
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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14.发明申请METHODS AND APPARATUSES FOR GENERATING A SEAL BETWEEN A CONDUIT AND A RESERVOIR WELL 审中-公开用于产生管道和储藏室之间的密封的方法和装置公开(公告)号：US20090146380A1
公开(公告)日：2009-06-11
申请号：US11719522
申请日：2006-08-10
CPC分类号： F04B19/006 , B01F5/0471 , B01F5/0646 , B01F5/0653 , B01F13/0059 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/565 , B01L2300/044 , B01L2300/0816 , B01L2300/1822 , B01L2300/1883 , B01L2300/1894 , B01L2400/0487 , B01L2400/0677
摘要： Methods and Apparatuses for Generating a Seal Between a Conduit and a Reservoir Well. According to one embodiment, an apparatus is provided for generating a seal between a conduit and a reservoir well. The apparatus can include a mount including a first and second end. The mount can also include a first aperture extending between the first and second ends. The apparatus can also include a tube including a first end engaging the first end of the mount, and operable to hold a conduit having an end such that the conduit extends through the first aperture of the mount and the end of the conduit communicates with a reservoir well. Further, the apparatus can include a nut operable to engage the mount and tube and seal the conduit to the first aperture of the mount such that air cannot communicate from the reservoir well through the first aperture of the mount.
摘要翻译： 在管道和储层之间产生密封的方法和装置。 根据一个实施例，提供了用于在导管和储存器井之间产生密封的装置。 该装置可以包括包括第一端和第二端的安装件。 安装件还可以包括在第一和第二端之间延伸的第一孔。 该装置还可以包括管，其包括接合安装件的第一端的第一端，并且可操作地保持具有端部的导管，使得导管延伸穿过安装件的第一孔，并且导管的端部与储存器 好。 此外，该装置可以包括可操作以接合安装件和管并且将导管密封到安装件的第一孔的螺母，使得空气不能从储存器穿过安装件的第一孔而连通。
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15.发明授权公开(公告)号：US06911132B2
公开(公告)日：2005-06-28
申请号：US10253368
申请日：2002-09-24
IPC分类号： B01F11/00 , B01F13/00 , B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N21/00 , G01N35/00 , G01N27/26 , B01D57/00
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
摘要翻译： 提供了一种用于操纵液滴的装置。 该装置是单面电极设计，其中所有导电元件都包含在其上操作液滴的一个表面上。 为了容纳待操作的液滴，可以与第一表面平行地设置另外的表面。 通过执行基于电润湿的技术来操纵液滴，其中包含在或嵌入在第一表面中的电极被顺序地通电并以受控的方式断电。 该装置能够进行多个液滴操纵过程，包括将两个液滴合并和混合在一起，将液滴分成两个或更多个液滴，通过从流动单独控制的液滴形成连续的液体流，以及液滴的迭代二进制或数字混合到 获得所需的混合比。
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公开(公告)号：US09631244B2
公开(公告)日：2017-04-25
申请号：US14131061
申请日：2012-06-26
IPC分类号： A61K39/00 , A61K9/14 , A61K38/20 , A61K38/21 , A61K39/39 , C12Q1/70 , B01L3/00 , B01L7/00 , C12Q1/68
CPC分类号： C12Q1/703 , B01L3/502784 , B01L3/502792 , B01L7/525 , B01L2200/0678 , B01L2200/16 , B01L2300/0816 , B01L2400/0427 , C12Q1/6806 , C12Q2523/308 , C12Q2527/125 , C12Q2531/113 , C12Q2563/159 , C12Q2565/607
摘要： A method of providing a droplet comprising one or more reagents, the method comprising, depositing a first aqueous droplet comprising the one or more reagents on a surface; drying the droplet to yield a dried composition on the surface comprising the one or more reagents; covering the dried composition with oil; and causing a second aqueous droplet in the oil to contact the dried composition and thereby resuspend one or more reagents.
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公开(公告)号：US08951732B2
公开(公告)日：2015-02-10
申请号：US12665788
申请日：2008-06-23
CPC分类号： C12Q1/6846 , C12Q2527/15 , C12Q2527/101
摘要： A method of amplifying a nucleic acid, the method comprising cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction.
摘要翻译： 一种扩增核酸的方法，所述方法包括使扩增准备的液滴通过温度梯度循环到适于在扩增反应中进行步骤的温度梯度内的位置。
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18.发明授权Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator 有权使用物理或化学特征的气泡形成技术来保持液滴致动器内的气泡公开(公告)号：US08877512B2
公开(公告)日：2014-11-04
申请号：US12692954
申请日：2010-01-25
CPC分类号： C07K1/26 , B01F13/0076 , B01F13/0084 , B01F13/0086 , B01L3/502723 , B01L3/502738 , B01L3/502792 , B01L2200/0673 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0427 , B01L2400/0688 , G01N2001/2244 , G01N2035/1034 , Y10T436/25
摘要： The present invention is directed to a droplet actuator and methods of making and using the droplet actuator including one or more substrates configured to form a droplet operations gap and including a physical or chemical feature that may be provided at a predetermined locus within or exposed to the droplet operations gap and configured to retain a bubble in position within the droplet operations gap.
摘要翻译： 本发明涉及液滴致动器以及制造和使用液滴致动器的方法，所述液滴致动器包括一个或多个被配置成形成液滴操作间隙的基板，并且包括物理或化学特征，该物理或化学特征可以设置在或 液滴操作间隙并且被配置为将气泡保持在液滴操作间隙内的适当位置。
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公开(公告)号：US08872527B2
公开(公告)日：2014-10-28
申请号：US12527208
申请日：2008-02-15
CPC分类号： B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
摘要： A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要翻译： 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。
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公开(公告)号：US08845872B2
公开(公告)日：2014-09-30
申请号：US13249425
申请日：2011-09-30
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
摘要翻译： 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。
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