Patent search ap:"Lubert Stryer" Page 3

21.

发明授权
Very large scale immobilized polymer synthesis 失效

公开(公告)号：US06566495B1

公开(公告)日：2003-05-20

申请号：US09465126

申请日：1999-12-17

Applicant: Stephen P. A. Fodor , Lubert Stryer , J. Leighton Read , Michael C. Pirrung

Inventor： Stephen P. A. Fodor , Lubert Stryer , J. Leighton Read , Michael C. Pirrung

IPC: C07K1600

CPC classification number: G01N21/6452 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07C229/14 , C07C229/16 , C07D263/44 , C07D317/62 , C07H19/073 , C07H19/173 , C07H21/04 , C07K1/042 , C07K1/045 , C07K1/047 , C07K1/062 , C07K7/06 , C07K17/06 , C07K17/14 , C12Q1/6809 , C12Q1/6816 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , C40B80/00 , G01N15/1475 , G01N21/253 , G01N21/6428 , G01N21/6458 , G03F7/00 , G03F7/0045 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , Y02P20/55 , Y10S435/969 , Y10S435/973 , Y10S436/807 , Y10S436/809

Abstract: A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.

22.

发明授权
Nucleotides and analogs having photoremoveable protecting groups 失效
Title translation: 具有光敏保护基团的核苷酸和类似物

公开(公告)号：US06310189B1

公开(公告)日：2001-10-30

申请号：US09490580

申请日：2000-01-25

Applicant: Stephen P. A. Fodor , Lubert Stryer , J. Leighton Read , Michael C. Pirrung

Inventor： Stephen P. A. Fodor , Lubert Stryer , J. Leighton Read , Michael C. Pirrung

IPC: C07H2100

CPC classification number: G01N21/6452 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07C229/14 , C07C229/16 , C07D263/44 , C07D317/62 , C07H19/04 , C07H19/10 , C07H21/00 , C07K1/042 , C07K1/045 , C07K1/047 , C07K7/06 , C07K17/06 , C07K17/14 , C12Q1/6809 , C12Q1/6816 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , G01N21/253 , G01N21/6428 , G03F7/00 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , Y02P20/55

Abstract: A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.

Abstract translation: 创造大规模化学多样性的综合战略。使用固相化学，光不稳定保护基团和光刻法来实现光定向空间可寻址并行化学合成。在一个实施例中使用二进制掩蔽技术。还公开了反应器系统，可光除保护组和改进的数据收集和处理技术。还提供了筛选接头分子的技术。

23.

发明授权
Printing molecular library arrays using deprotection agents solely in the vapor phase 失效
Title translation: 使用去保护剂仅在气相中印刷分子库阵列

公开(公告)号：US5599695A

公开(公告)日：1997-02-04

申请号：US395604

申请日：1995-02-27

Applicant: R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler

Inventor： R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler

IPC: B01J19/00 , B41M3/00 , C07H21/00 , C40B40/06 , C40B50/14 , C40B60/14 , G03F7/00 , C12P19/34 , C07H21/02 , C07K5/00 , G01N33/543

CPC classification number: C07H21/00 , B01J19/0046 , G03F7/00 , B01J2219/00378 , B01J2219/00382 , B01J2219/00385 , B01J2219/00427 , B01J2219/00454 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00722 , B41M3/00 , C40B40/06 , C40B50/14 , C40B60/14

Abstract: A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.

Abstract translation: 一种用于选择性地将印刷材料施加到基底上以在基底的选定区域合成寡核苷酸阵列的方法和装置。印刷材料包括阻挡材料，单体序列，核苷，去保护剂，载体材料等。该方法和装置还依赖于基于标准的基于DMT的化学，以及气相脱保护剂如固体TCA等。

24.

发明授权
Spatially-addressable immobilization of anti-ligands on surfaces 失效

公开(公告)号：US5482867A

公开(公告)日：1996-01-09

申请号：US54121

申请日：1993-04-23

Applicant: Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg

Inventor： Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg

IPC: C07D233/38 , C07D491/048 , C07D495/04 , C40B40/06 , G01N33/543 , G01N33/552

CPC classification number: C07D495/04 , C07D233/38 , G01N33/54306 , G01N33/54353 , G01N33/552 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , Y02P20/55

Abstract: Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.

25.

发明授权
Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof 失效
Title translation: 聚合物和受体结合筛选的大规模光刻胶固相合成

公开(公告)号：US5143854A

公开(公告)日：1992-09-01

申请号：US492462

申请日：1990-03-07

Applicant: Michael C. Pirrung , J. Leighton Read , Stephen P. A. Fodor , Lubert Stryer

Inventor： Michael C. Pirrung , J. Leighton Read , Stephen P. A. Fodor , Lubert Stryer

IPC: G01N33/48 , B01J19/00 , B01J19/08 , B01L7/00 , B05D3/00 , C07B51/00 , C07B61/00 , C07C229/14 , C07C229/16 , C07C231/02 , C07C237/22 , C07D263/44 , C07D317/62 , C07H19/04 , C07H19/10 , C07H21/00 , C07K1/04 , C07K1/06 , C07K7/06 , C07K14/00 , C07K16/00 , C07K17/02 , C07K17/06 , C07K17/08 , C07K17/14 , C08F2/46 , C12M1/34 , C12Q1/68 , C40B30/04 , C40B40/06 , C40B40/10 , C40B60/14 , G01N1/30 , G01N15/14 , G01N21/25 , G01N21/64 , G01N33/53 , G01N33/533 , G01N33/536 , G01N33/541 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , G03F7/00 , G03F7/26 , G03F7/38 , G11C13/02 , H01L21/98

CPC classification number: C08G69/00 , B01J19/0046 , B01L7/52 , B82Y10/00 , B82Y30/00 , C07C229/14 , C07C229/16 , C07D263/44 , C07D317/62 , C07H19/04 , C07H19/10 , C07H21/00 , C07K1/04 , C07K1/042 , C07K1/045 , C07K1/047 , C07K1/062 , C07K17/06 , C07K17/14 , C07K7/06 , C12Q1/6809 , C12Q1/6816 , C12Q1/6837 , C12Q1/6874 , C40B80/00 , G01N21/253 , G01N21/6428 , G01N21/6452 , G03F7/00 , G03F7/26 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , H01L25/50 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00639 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , H01L2924/0002 , Y02P20/55 , Y10S435/961 , Y10S435/968 , Y10S435/969 , Y10S435/973 , Y10S436/807 , Y10S436/809 , Y10T428/31971 , Y10T436/2575

Abstract: Polypeptide arrays can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoremovable group to the activated regions, and repeating the steps of activation and attachment until polypeptides of the desired length and sequences are synthesized. The resulting array can be used to determine which peptides on the array can bind to a receptor.

Abstract translation: 可以在基底上合成多肽阵列，通过将可光去除的基团附着到基底的表面，将基底的选定区域暴露于光以活化那些区域，将具有可光去除基团的氨基酸单体连接到活化区域，并重复步骤的活化和附着，直到合成所需长度和序列的多肽。得到的阵列可用于确定阵列上的哪些肽可以结合受体。

26.

发明授权
High sensitivity fluorescent single particle and single molecule detection apparatus and method 失效
Title translation: 高灵敏度荧光单粒子和单分子检测仪器及方法

公开(公告)号：US4979824A

公开(公告)日：1990-12-25

申请号：US358782

申请日：1989-05-26

Applicant: Richard A. Mathies , Konan Peck , Lubert Stryer

Inventor： Richard A. Mathies , Konan Peck , Lubert Stryer

IPC: G01N21/63 , G01N15/02 , G01N15/14 , G01N21/64 , G01N33/543

CPC classification number: G01N21/645 , G01N15/147 , G01N15/1429 , G01N15/1475 , G01N2015/0222 , G01N21/6456

Abstract: Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.

Abstract translation: 描述了用于在流体或基板上的单个荧光分子极限处的超灵敏检测的装置，其包括用于照射预定体积的基板的流体或区域的装置，从而发射包括来自流体的背景光的光，以及从位于该地区的颗粒中发射光子。实时检测光子脉冲以产生输出代表信号。信号被接收，并且来自荧光颗粒的能量爆发与背景能区别开来，以提供颗粒或分子的数量，位置或浓度的指示。

27.

发明申请
Chip-based speciation and phenotypic characterization of microorganisms 有权
Title translation: 基于芯片的形态和微生物的表型表征

公开(公告)号：US20060204970A1

公开(公告)日：2006-09-14

申请号：US11050647

申请日：2005-02-04

Applicant: Thomas Gingeras , David Mack , Mark Chee , Anthony Berno , Lubert Stryer , Ghassan Ghandour , Ching Wang

Inventor： Thomas Gingeras , David Mack , Mark Chee , Anthony Berno , Lubert Stryer , Ghassan Ghandour , Ching Wang

IPC: C12Q1/68

CPC classification number: C40B80/00 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , C12Q1/6809 , C12Q1/6837 , C12Q1/6888 , C12Q1/689 , C12Q2600/156 , G01N33/54386 , G06F19/20 , C12Q2565/501

Abstract: This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.

Abstract translation: 本发明提供了基于寡核苷酸的阵列和用于指定和表型生物的方法，例如使用基于结核分枝杆菌rpoB基因的寡核苷酸序列。可以通过将来自生物体的靶核酸的杂交模式与数据库中的杂交模式进行比较来确定生物体所属的组或物种。