公开(公告)号：US20240302738A1

公开(公告)日：2024-09-12

申请号：US18667993

申请日：2024-05-17

申请人： ILLUMINA, INC.

发明人： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC分类号： G03F7/00 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/004 ,  G03F7/075 ,  G03F7/16

CPC分类号： G03F7/0002 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0046

摘要： An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20240301495A1

公开(公告)日：2024-09-12

申请号：US18632703

申请日：2024-04-11

申请人： Natera, Inc.

发明人： MATTHEW RABINOWITZ ,  Milena BANJEVIC ,  Zachary DEMKO ,  David JOHNSON ,  Dusan KIJACIC ,  Dimitri PETROV ,  Joshua SWEETKIND-SINGER ,  Jing XU

IPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00

CPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/149 ,  C12Q2545/114 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158

摘要： Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents.

公开(公告)号：US20240301490A1

公开(公告)日：2024-09-12

申请号：US18195049

申请日：2023-05-09

申请人： Zafrens, Inc.

发明人： Kandaswamy Vijayan

IPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

摘要： A method of cellular analysis at high throughput is provided. More specifically, the invention relates to capturing single cells, culturing single cells to generate clonal copies of single cells, perturbing the single cells or the clonal copies derived from the single cells, performing experiments on single cells or clonal copies of single cells, imaging cells, capturing genomic content and other material from cells and being able to relate the captured genomic information to the functional and imaging analysis performed on the same cell.

公开(公告)号：US20240301466A1

公开(公告)日：2024-09-12

申请号：US18578523

申请日：2022-07-13

申请人： University of Washington

发明人： Stephen J. SALIPANTE

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869

CPC分类号： C12Q1/6806 ,  C12N15/1065 ,  C12Q1/6869

摘要： Embodiments of the present disclosure provide a method for detecting one or more genetic variants in a biological sample. Embodiments of the method include preparing an error-corrected nucleic library for sequencing, wherein the nucleic acid library comprises a double stranded nucleic molecule comprising a hairpin adapter, wherein the hairpin adapter covalently joins each strand of the double stranded nucleic molecule into a single covalently linked duplex strand for self-correction of sequencing errors.

公开(公告)号：US20240299944A1

公开(公告)日：2024-09-12

申请号：US18664102

申请日：2024-05-14

申请人： Illumina, Inc.

发明人： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC分类号： B01L3/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N1/28 ,  G01N15/10 ,  G01N33/543

CPC分类号： B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/54333 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N2015/1006

摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US20240299884A1

公开(公告)日：2024-09-12

申请号：US18604714

申请日：2024-03-14

申请人： Oxford Nanopore Technologies PLC

发明人： Ping Xie ,  Ken Healy ,  Justin Millis

IPC分类号： B01D67/00 ,  B01D69/02 ,  B01D69/06 ,  B01D69/12 ,  B82Y40/00 ,  C12Q1/6869 ,  C25F3/02 ,  C25F3/14 ,  C25F7/00 ,  G01N33/487

CPC分类号： B01D67/0062 ,  B01D69/02 ,  B01D69/06 ,  B01D69/12 ,  B01D69/1214 ,  C12Q1/6869 ,  C25F3/02 ,  C25F3/14 ,  C25F7/00 ,  G01N33/48721 ,  B01D2323/42 ,  B01D2325/021 ,  B01D2325/06 ,  B82Y40/00

摘要： Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.

公开(公告)号：US12087402B2

公开(公告)日：2024-09-10

申请号：US15772389

申请日：2016-10-28

申请人： KONINKLIJKE PHILIPS N.V.

发明人： Brian David Gross ,  Thomas Chou ,  Saeed Babaeizadeh ,  Pradyumna Dutta ,  Andrew Arthur ,  Henry Lin

IPC分类号： G16B30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/689 ,  G06F17/18 ,  G16B10/00 ,  G16B20/00 ,  G16B30/10 ,  G16B40/20 ,  G16B50/00

CPC分类号： G16B30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/689 ,  G06F17/18 ,  G16B10/00 ,  G16B20/00 ,  G16B30/10 ,  G16B40/20 ,  G16B50/00

摘要： Provided herein are computer implemented methods, systems and processes for determining a transmission metric or transmission path for related pathogens. Also provided herein is a non-transitory computer-readable storage medium with an executable program stored thereon, which program is configured to instruct a microprocessor to generate a transmission path for related pathogens.

公开(公告)号：US12085558B2

公开(公告)日：2024-09-10

申请号：US17176371

申请日：2021-02-16

申请人： The California State University—Northridge

发明人： Hendrik Postma

IPC分类号： G01N33/487 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/327

CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  G01N27/3278 ,  B82Y15/00

摘要： Processes for conductive material nanogap formation have been developed that include: providing a base material, wherein the base material is either solid or comprises a micropore that extends through the first layered material, and wherein the micropore comprises a top opening, a bottom opening, and a volume boundary, applying a conductive material sheet to the first layered material, wherein the conductive material sheet covers the top opening of the micropore, applying two conducting electrodes to the conductive material sheet, so that each one of the conducting electrodes is positioned on either side of the micropore, applying an etch mask that covers at least a part of the conductive material sheet, the top opening of the micropore (if present), or a combination thereof, applying a passivation layer over at least the etch mask, fabricating a hole in the passivation layer directly above the top opening of the micropore, and applying at least one voltage pulse through the at least one conducting electrode to create a nanogap in the conductive material sheet, wherein the nanogap is over and open to the top opening of the micropore. In some embodiments, the micropore and the nanogap are fabricated simultaneously.

公开(公告)号：US12084652B2

公开(公告)日：2024-09-10

申请号：US17705115

申请日：2022-03-25

申请人： Viome Life Sciences, Inc.

发明人： Momchilo Vuyisich ,  Andrew Hatch ,  Ryan Toma ,  Brittany Twibell ,  James Horne ,  Kinga Canfield

IPC分类号： C12N15/10 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/689 ,  C40B40/06 ,  C40B40/08

CPC分类号： C12N15/1068 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6844 ,  C12Q1/689 ,  C40B40/06 ,  C40B40/08 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q2527/143

摘要： Provided herein are methods and composition for processing samples that contain nucleic acids, or cells containing nucleic acids, of a microbiome, using amounts of primers within a range of mole values and rounds of polymerase chain reaction (PCR) within a range of numbers of rounds.

公开(公告)号：US20240294984A1

公开(公告)日：2024-09-05

申请号：US18618588

申请日：2024-03-27

申请人： GUARDANT HEALTH, INC.

发明人： Sante GNERRE

IPC分类号： C12Q1/6876 ,  C12Q1/6869

CPC分类号： C12Q1/6876 ,  C12Q1/6869 ,  C12Q2600/156

摘要： Provided herein are methods and systems for allele typing and variant calling.