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公开(公告)号：US07576037B2
公开(公告)日：2009-08-18
申请号：US11282800
申请日：2005-11-18
CPC分类号： C40B60/14 , B01J19/0046 , B01J2219/00286 , B01J2219/00466 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00592 , B01J2219/00596 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725
摘要： Apparatus and methods are described for split synthesis combinatorial chemistry that provides candidate libraries where an even distribution of theoretical products is obtainable through even mixing during the pooling step, followed by controlled redistribution of the mixed pooled products from the prior addition step into separate synthesis columns, one for each different specie of subunit to be added.
摘要翻译： 描述了用于分裂合成组合化学的装置和方法，其提供候选文库，其中理论产物的均匀分布可以通过在汇集步骤期间的均匀混合获得，随后将混合的合并产物从先前加入步骤重新分配到单独的合成柱中， 一个用于每个不同种类的待添加的亚单位。
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公开(公告)号：US20080020945A1
公开(公告)日：2008-01-24
申请号：US11478548
申请日：2006-06-29
申请人： Roderick Hyde , Edward Jung , Lowell Wood
发明人： Roderick Hyde , Edward Jung , Lowell Wood
IPC分类号： C40B60/08
摘要： Methods, apparatus, systems, computer programs and computing devices related to biologically assembling and/or synthesizing peptides and/or proteins are disclosed.
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公开(公告)号：US20240318353A1
公开(公告)日：2024-09-26
申请号：US18353653
申请日：2023-07-17
发明人： Chenli Liu , Wangsheng Lai , Qian Chen , Ting Wei , Chenjian Sun
摘要： A visual continuous spatial directed evolution method is disclosed. The host grows and moves in a solid culture space, the host carrying a foreign target gene to be evolved and containing a gene element that assists the evolution of the target gene, the target gene being correlated with the growth and movement of the host. Depending on different spatial distribution patterns formed in the solid culture space during the growth and movement of the host, screening is performed to obtain an evolved product. This method is carried out directly in the solid culture space. Depending on images of different spatial distribution morphologies visible to the naked eye that are locally formed, selection of evolved products is performed without the need for liquid fed-batch culture equipment. In addition, the evolution effect is visually observed through the infection spots formed during evolution, so that no real-time monitoring equipment is required.
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公开(公告)号：US11702764B2
公开(公告)日：2023-07-18
申请号：US16904549
申请日：2020-06-17
发明人： Chenli Liu , Wangsheng Lai , Qian Chen , Ting Wei , Chenjian Sun
摘要： A visual continuous spatial directed evolution method is disclosed. The host grows and moves in a solid culture space, the host carrying a foreign target gene to be evolved and containing a gene element that assists the evolution of the target gene, the target gene being correlated with the growth and movement of the host. Depending on different spatial distribution patterns formed in the solid culture space during the growth and movement of the host, screening is performed to obtain an evolved product. This method is carried out directly in the solid culture space. Depending on images of different spatial distribution morphologies visible to the naked eye that are locally formed, selection of evolved products is performed without the need for liquid fed-batch culture equipment. In addition, the evolution effect is visually observed through the infection spots formed during evolution, so that no real-time monitoring equipment is required.
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公开(公告)号：US20180355350A1
公开(公告)日：2018-12-13
申请号：US15996222
申请日：2018-06-01
发明人： Darren Roy Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： C12N15/10 , G01N33/68 , B01F13/00 , G01N33/543 , G01N33/536 , C40B70/00 , C40B60/12 , C40B60/08 , C40B50/08 , C40B40/04 , C40B30/04 , C12Q1/6876 , C12Q1/6874 , C12Q1/686 , C07K1/04 , B82Y30/00 , B82Y5/00 , B01L3/00 , B01J19/00 , G01N33/542 , G01N21/64 , C12Q1/6869 , C12Q1/6834 , C12Q1/6825 , C12Q1/6816 , C12Q1/6804 , B01L7/00 , B01F3/08
CPC分类号： C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
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公开(公告)号：US09962677B2
公开(公告)日：2018-05-08
申请号：US15669080
申请日：2017-08-04
发明人： Leroy Cronin
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00353 , B01J2219/00479 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00689 , B01J2219/00695 , B01J2219/00698 , B01J2219/007 , B01J2219/00702 , B01J2219/00704 , B01J2219/00738 , B01J2219/00747 , B01J2219/00756 , C40B50/04 , C40B60/08
摘要： The invention provides a method for preparing a compound or a product having one or more characteristics that meet or exceed a user specification, the process comprising the step of selecting a first combination of chemical inputs, optionally together with physical inputs, and supplying those inputs to a reaction space, thereby to generate a first product; analyzing one or more characteristics of the product generated; comparing the one or more characteristics against a user specification; using a genetic algorithm selecting a second combination of chemical inputs, optionally together with physical inputs, wherein the second combination differs from the first combination, and supplying those inputs to the reaction space, thereby to generate a second product; analyzing one or more characteristics of the second product generated; comparing the one or more characteristics generated against the user specification; repeating the selecting and analyzing steps for further individual combinations of chemical and/or physical inputs, to provide an array of products wherein the flow chemistry system operates continuously to provide the first, second and further products, thereby to identify one or more products meeting or exceeding the user specification.
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67.发明授权Microfluidic devices and methods of use in the formation and control of nanoreactors 有权微流体装置和用于形成和控制纳米反应器的方法公开(公告)号：US09328344B2
公开(公告)日：2016-05-03
申请号：US13759660
申请日：2013-02-05
发明人： Darren Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： C12Q1/68 , G01N33/543 , B01L3/00 , C12N15/10 , B01F13/00 , B01J19/00 , B82Y5/00 , B82Y30/00 , C07K1/04 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N33/536 , G01N33/68 , B01F3/08 , B01L7/00
CPC分类号： C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.
摘要翻译： 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 这样的方法可以包括通过将化合物的水溶液和独特的液体标签的水溶液乳化在微流体装置上来标记化合物文库，所述微流体装置包括整体地布置在微细加工的基底上的多个可电寻址的通道支承的流体模块， 提供了用于不混溶流体流动的通道，由此每种化合物用独特的液体标记标记，汇集标记的乳液，将标记的乳液与含有特定细胞或酶的乳液聚结，从而形成纳米反应器，筛选纳米反应器以获得所需的反应 在纳米反应器的内容物之间，并解码液体标签，从而从化合物文库鉴定单一化合物。
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公开(公告)号：US08906831B2
公开(公告)日：2014-12-09
申请号：US12384097
申请日：2009-03-30
申请人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig , Jeremy Gray , Robert Sebra , Lei Sun , Stephen Dudek
发明人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig , Jeremy Gray , Robert Sebra , Lei Sun , Stephen Dudek
CPC分类号： B01J19/0046 , B01J2219/00596 , C12N2310/50 , C12N2310/51 , C12Q1/6869 , C12Q2535/122 , C40B40/06 , G01N33/54313 , G01N33/54366
摘要： Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.
摘要翻译： 提供了将单一分析物分子非随机加载到阵列结构中的方法。 所述方法允许将目标分子群体分配到多个尺寸的限制区域例如孔中。 上限部分与单独的靶分子连接。 尺寸部分相对于尺寸限制的反应或观察区域具有足够的尺寸，使得只有选定数量的施胶部分将适合尺寸限制区域。 限制区域和施胶部分或靶分子包括允许控制施胶剂的载荷的选定的电荷。
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69.发明授权Polymorph, polymorph screening system, and polymorph preparing and screening method 有权多态性，多态性筛选系统，多态性准备筛选方法公开(公告)号：US08865093B2
公开(公告)日：2014-10-21
申请号：US13277446
申请日：2011-10-20
申请人： Tu Lee , Shih-Chia Chang , Jen-Fan Peng , Pu-Yun Wang , Yu-Kun Lin
发明人： Tu Lee , Shih-Chia Chang , Jen-Fan Peng , Pu-Yun Wang , Yu-Kun Lin
CPC分类号： B01J19/0046 , B01J2219/00495 , B01J2219/00533 , B01J2219/00585 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00637 , B01J2219/00702 , B01J2219/0072 , B01J2219/00756 , C40B60/08 , G01N33/15
摘要： A polymorph, a polymorph screening system, and a polymorph preparing and screening method are disclosed. The polymorph preparing and screening method includes the following steps: providing a plurality of substrates of different materials; causing an organic material to grow crystals on the plurality of substrates through solution-cooling crystallization process, so that a plurality of polymorphs with different characteristics are prepared; and screening the plurality of polymorphs prepared in the previous step to obtain polymorphs with desired characteristics.
摘要翻译： 披露了一种多晶型物，一种多晶型筛选系统和一种多晶型制备和筛选方法。 多晶型制备和筛选方法包括以下步骤：提供多种不同材料的基材; 使有机材料通过溶液 - 冷却结晶过程在多个基板上生长晶体，从而制备具有不同特性的多个多晶型物; 并筛选前一步骤制备的多个多晶型物，以获得具有所需特征的多晶型物。
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70.发明申请公开(公告)号：US20130090260A1
公开(公告)日：2013-04-11
申请号：US13708778
申请日：2012-12-07
申请人： Michael P. Nova , Andrew E. Senyei
发明人： Michael P. Nova , Andrew E. Senyei
CPC分类号： C40B30/04 , B01J19/0046 , B01J2219/00295 , B01J2219/00299 , B01J2219/00308 , B01J2219/00333 , B01J2219/00344 , B01J2219/00461 , B01J2219/00463 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00542 , B01J2219/00547 , B01J2219/00549 , B01J2219/00551 , B01J2219/0056 , B01J2219/00563 , B01J2219/00567 , B01J2219/00569 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L3/5027 , B01L3/502707 , B01L2300/0609 , B82Y10/00 , C07K1/00 , C07K1/04 , C07K1/047 , C40B40/06 , C40B40/10 , C40B60/08 , C40B60/14 , C40B70/00 , G01N35/00732 , G01N35/00871 , G01N2015/149 , G01N2035/00782 , G11C13/0014 , G11C13/0019 , G11C13/025
摘要： In a multiplexed assay, each molecule of a plurality of molecules is attached to a support matrix particle with a substrate adapted for attachment and/or synthesis of molecules. A spectrally-encoded identifier embodied in a photochemical medium is embedded or encased within the substrate to uniquely identify the molecule attached to the substrate. The molecules are exposed to one or more processing conditions, and then placed within the path of an optical detector adapted to read the spectrally-encoded identifier and measure biochemical activity on each support matrix particle. The measured biochemical activity is associated with the unique identity of the support matrix particle and, hence, with the molecule attached to the particle.
摘要翻译： 在多重测定中，多个分子的每个分子连接到具有适于附着和/或合成分子的底物的载体基质颗粒上。 在光化学介质中实施的经光谱编码的标识符被嵌入或封装在衬底内以唯一地识别附着于衬底的分子。 分子暴露于一个或多个处理条件，然后放置在适于读取频谱编码的标识符的光学检测器的路径内，并测量每个支持矩阵粒子上的生化活性。 测量的生物化学活性与支持基质颗粒的独特特性相关联，因此与附着于颗粒的分子相关。
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