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    Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray
    72.
    发明申请
    Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray 审中-公开
    在微阵列上使用酶反应的核酸杂交和检测

    公开(公告)号：US20110003703A1

    公开(公告)日：2011-01-06

    申请号：US12496613

    申请日：2009-07-01

    申请人： Charles Ma ,  Yulin Lee

    发明人： Charles Ma ,  Yulin Lee

    IPC分类号： C40B30/04 ,  C12Q1/68 ,  C40B60/12

    CPC分类号： C40B80/00 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00637 ,  C12Q1/6837 ,  C12Q2600/178 ,  C40B50/18 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2533/101

    摘要： Embodiments are directed to a methods and systems for nucleic acid detection using enzymatic reactions on a microarray. In one embodiment, a probe comprising a probe nucleotide sequence and a substantially homogenous sequence extender portion is provided on the surface of a microarray. The probe nucleotide sequence is hybridized to the complementary target nucleotide sequence. A solution containing enzymes and detection elements is applied to the hybridized probe structure. The enzyme determines the composition of the nucleotide structure of the extender and creates a complementary homogenous sequence extender structure between the target nucleotide sequence and the microarray surface structure. The detection elements in the solution are bound to the extender structure, thus allowing detection using an appropriate detector system.

    摘要翻译： 实施方案涉及使用微阵列上的酶反应进行核酸检测的方法和系统。 在一个实施方案中，在微阵列的表面上提供包含探针核苷酸序列和基本均匀的序列延伸部分的探针。 探针核苷酸序列与互补靶核苷酸序列杂交。 将含有酶和检测元素的溶液应用于杂交探针结构。 该酶决定了扩增体的核苷酸结构的组成，并在目标核苷酸序列和微阵列表面结构之间产生互补的同源序列扩增结构。 解决方案中的检测元件结合到扩展器结构，从而允许使用适当的检测器系统进行检测。

    SYSTEMS AND METHODS FOR POINT-OF-CARE AMPLIFICATION AND DETECTION OF POLYNUCLEOTIDES
    75.
    发明申请
    SYSTEMS AND METHODS FOR POINT-OF-CARE AMPLIFICATION AND DETECTION OF POLYNUCLEOTIDES 有权
    关键点放大和多核苷酸检测的系统和方法

    公开(公告)号：US20100285479A1

    公开(公告)日：2010-11-11

    申请号：US12779397

    申请日：2010-05-13

    申请人： Robert Delmar Jenison

    发明人： Robert Delmar Jenison

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C12Q1/6844 ,  C12Q2527/107 ,  C12Q2523/313 ,  C12Q2521/513 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2537/101 ,  C12Q2565/519

    摘要： Composition and methods for amplifying and detecting solution-state polynucleotide targets in a single device are described. In one aspect, a method for a coupled isothermal amplification and detection process utilizes a coated solid support, including a solid substrate, a cationic layer, and a plurality of target-specific probes attached to the coated solid support. Polynucleotide targets in the sample are amplified by an isothermal amplification process involving in situ hybridization onto the coated solid support. The entire process can be carried out with a high degree of specificity under low salt conditions in less than one hour. Further aspects of the present invention include methods for coupled hybridization/detection of polynucleotide targets, coated silicon biosensors optimized for use with the coupled detection systems to provide visual detection of polynucleotide targets under visible light conditions, and kits for practicing in the above described methods.

    摘要翻译： 描述了用于在单个装置中扩增和检测溶液状态多核苷酸靶标的组合物和方法。 在一个方面，用于偶联等温放大和检测方法的方法利用包被的固体支持物，包括固体底物，阳离子层和连接到涂覆的固体支持物上的多个靶特异性探针。 样品中的多核苷酸靶标通过等温扩增过程进行扩增，包括原位杂交到涂覆的固体支持物上。 整个过程可以在低盐条件下在低于1小时内以高度的特异性进行。 本发明的其它方面包括用于多核苷酸靶的偶联杂交/检测的方法，优化用于与偶联检测系统一起使用的涂覆的硅生物传感器以提供可见光条件下的多核苷酸靶的视觉检测的方法，以及用于在上述方法中实践的试剂盒。

    Microarray and uses therefor
    76.
    发明授权
    Microarray and uses therefor 有权
    微阵列和用途

    公开(公告)号：US07794946B1

    公开(公告)日：2010-09-14

    申请号：US09245615

    申请日：1999-02-04

    申请人： James P. Hoeffler ,  Joseph M. Fernandez ,  Marc S. Nasoff

    发明人： James P. Hoeffler ,  Joseph M. Fernandez ,  Marc S. Nasoff

    IPC分类号： G01N33/53 ,  G01N33/566 ,  B01L3/00

    CPC分类号： G01N33/548 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  C12Q1/6837 ,  G01N33/543 ,  G01N33/54393 ,  G01N33/6845 ,  G01N33/6854

    摘要： The invention disclosed herein comprises methods of using microarrays to simplify analysis and characterization of genes and their function. In one aspect of the invention the methods are used to identify and characterize antibodies having binding affinity for a specific target antigen. The invention further comprises a method of determining gene expression at the protein level comprising contacting an array of characterized or uncharacterized antibodies on a solid surface with one or more proteins and identifying the antibodies to which said protein(s) binds. This method can be additionally used to compare the protein expression in two different populations of cells, such as normal cells and cancer cells or resting cells and stimulated cells. A further aspect of the invention comprises a method of determining gene expression at the protein level comprising contacting a microarray of nucleic acid samples derived from a variety of different sources with one or more nucleic acid probes then identifying the sample or samples to which the probe binds.

    摘要翻译： 本文公开的发明包括使用微阵列简化基因的分析和表征及其功能的方法。 在本发明的一个方面，所述方法用于鉴定和表征对特异性靶抗原具有结合亲和力的抗体。 本发明还包括确定蛋白质水平的基因表达的方法，包括将固体表面上的特征或未表征的抗体阵列与一种或多种蛋白质接触，并鉴定所述蛋白质与之结合的抗体。 这种方法可以另外用于比较两种不同细胞群体中的蛋白质表达，如正常细胞和癌细胞，休眠细胞和刺激细胞。 本发明的另一方面包括确定蛋白质水平的基因表达的方法，其包括将衍生自多种不同来源的核酸样品的微阵列与一种或多种核酸探针接触，然后鉴定探针结合的样品或样品 。