公开(公告)号：US20180334670A1

公开(公告)日：2018-11-22

申请号：US16031880

申请日：2018-07-10

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Michael Schnall-Levin ,  Anthony Makarewicz ,  Steven Short

IPC: C12N15/10 ,  G01N15/14 ,  B01L3/00 ,  G01N21/64

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/086 ,  C12N15/1006 ,  C12N15/1075 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2565/629 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1404 ,  G01N15/1484 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  C12Q2535/122 ,  C12Q2531/113 ,  C12Q2563/113 ,  C12Q2563/159 ,  C12Q2563/143

Abstract: Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.

公开(公告)号：US20180263602A1

公开(公告)日：2018-09-20

申请号：US15760453

申请日：2016-09-19

Applicant: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS ,  FUNDACION PARA EL CONOCIMIENTO MADRI+D ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Luis ELVIRA SEGURO ,  Oscar MARTINEZ GRAULLERA ,  Shiva KANT SHUKLA ,  Francisco MONTERO ,  Javier JIMENEZ ,  Ian Richard BUTTERWORTH ,  Brian ANTHONY ,  John HEASON LEE ,  Carlos CASTRO GONZALEZ

IPC: A61B8/08 ,  A61B8/00 ,  G01N15/02 ,  G01N15/10 ,  G01N29/032

CPC classification number: A61B8/5223 ,  A61B8/0891 ,  A61B8/58 ,  G01N15/02 ,  G01N15/0211 ,  G01N15/1056 ,  G01N29/02 ,  G01N29/032 ,  G01N2015/0687 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1087 ,  G01N2291/02466

Abstract: The present invention relates to a method for measuring circulating cells in superficial body fluids by means of high-frequency-based device. The method can be used for detecting circulating cells in the fluids of an individual without the necessity of extracting a sample of the individual, being useful as a diagnostic tool and for monitoring the effectiveness of a treatment administered to an individual suffering from a viral, protozoal, fungal and/or bacterial disease.

公开(公告)号：US10073024B2

公开(公告)日：2018-09-11

申请号：US14439429

申请日：2013-10-29

Applicant: The Regents Of The University Of Michigan

Inventor： Sunitha Nagrath ,  Hyeun Joong Yoon ,  Eric Lin ,  Max S. Wicha ,  Lianette Rivera Baez ,  Diane M. Simeone

IPC: G01N15/10 ,  B01L3/00 ,  G01N33/50 ,  G01N33/574 ,  G01N15/02 ,  C12Q1/04 ,  G01N15/00

CPC classification number: G01N15/1056 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  G01N15/0272 ,  G01N33/5005 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: A microfluidic device for detecting rare cells in a fluid sample comprises the rare cell and other cells. The microfluidic device comprises an inlet for receiving the fluid sample, a labyrinth channel structure in fluid communication with the inlet, and an outlet in fluid communication with the labyrinth channel structure for collecting the rare cells separated from the other cells in the fluid sample. The labyrinth channel structure comprises at least one channel through which the fluid sample flows. The at least one channel has a plurality of segments and a plurality of corners with each corner defined between adjacent segments. The presence of the plurality of corners induces separation of the rare cells from the other cells in the fluid sample as the rare cells move to a first equilibrium position within the at least one channel when a ratio of inertial lift forces (FZ) and Dean flow (FD) of the fluid sample is from 2 to 10.

公开(公告)号：US20180029035A1

公开(公告)日：2018-02-01

申请号：US15660606

申请日：2017-07-26

Applicant: Ancera Corp.

Inventor： Hur KOSER ,  G. Thomas ROTH

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/502707 ,  B01D19/0031 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/10 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/082 ,  B03C1/0335 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/20 ,  B03C2201/26 ,  B07B1/4636 ,  G01N15/1031 ,  G01N15/1056 ,  G01N21/6452 ,  G01N35/1097 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: In certain embodiments, the disclosure provides an inflatable bladder lid that configures with a cartridge configured for assay testing. The inflatable bladder provides substantially uniform pressure to the cartridge. The pressure is substantially distributed across the one or more regions of the cartridge to extend pressure over a wide cartridge surface. At least a portion of the bladder lid may comprise a flexible membrane material that inflates and stretches over at least a portion of the cartridge to conformally contact its first/top surface.

公开(公告)号：US20170343467A1

公开(公告)日：2017-11-30

申请号：US15483309

申请日：2017-04-10

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Emanuel Tito Mendes Machado ,  Blair D. Morad ,  Rudolf Hulspas ,  Donald Francis Perrault, JR.

IPC: G01N15/14 ,  G01N21/53 ,  G01N15/10 ,  G01N21/64

CPC classification number: G01N15/1484 ,  G01N15/1012 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/64 ,  G01N2015/1018 ,  G01N2015/1075 ,  G01N2015/1402 ,  G01N2015/149

Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.

公开(公告)号：US20170248508A1

公开(公告)日：2017-08-31

申请号：US15595548

申请日：2017-05-15

Applicant: GPB SCIENTIFIC, LLC ,  UNIVERSITY OF MARYLAND, BALTIMORE ,  THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Anthony WARD ,  Khushroo GANDHI ,  Alison SKELLEY ,  Curt CIVIN ,  James C. STURM ,  Lee AURICH ,  Michael GRISHAM ,  Joseph D'SILVA ,  Roberto CAMPOS-GONZALEZ ,  Robert H. Austin

IPC: G01N15/10 ,  G01N33/574 ,  G01N35/00 ,  B01L3/00

CPC classification number: G01N15/1056 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/082 ,  B01L2400/086 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/5091 ,  G01N33/574 ,  G01N33/6893 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/142 ,  G01N2015/149

Abstract: Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.

公开(公告)号：US09733238B2

公开(公告)日：2017-08-15

申请号：US13985383

申请日：2012-02-09

Applicant: Steven Charles Deane

Inventor： Steven Charles Deane

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B01L3/00 ,  G01N33/50 ,  G01N15/10 ,  G01N1/38

CPC classification number: G01N33/5094 ,  B01L3/5027 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  G01N15/1056 ,  G01N2001/383 ,  Y10T29/49117

Abstract: A measurement chip (100) is disclosed for use with a microfluidic resistance network (20) comprising a microfluidic sample preparation stage (34, 38), a sample outlet (42) and a waste outlet (44) both in fluidic communication with said preparation stage. The measurement chip comprises a sample channel (104) for receiving a sample from said sample outlet (42), the sample channel comprising measurement means (120, 130) and having a first fluidic resistance; and a waste channel (114) for receiving a waste stream from said waste outlet (44) and having a second fluidic resistance.

公开(公告)号：US20170136463A1

公开(公告)日：2017-05-18

申请号：US15355696

申请日：2016-11-18

Applicant: California Institute of Technology

Inventor： Yu-Chong Tai ,  Siyang Zheng ,  Jeffrey Chun-Hui Lin ,  Harvey L. Kasdan

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2400/086 ,  G01N15/1056 ,  G01N15/1484 ,  G01N33/5005 ,  G01N33/5094 ,  G01N33/582 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/13 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.

公开(公告)号：US09643181B1

公开(公告)日：2017-05-09

申请号：US15017998

申请日：2016-02-08

Applicant: International Business Machines Corporation

Inventor： Hung-Yang Chang ,  Ning Li ,  Fei Liu ,  Qiqing C. Ouyang ,  Seshadri Subbanna ,  Yajuan Wang

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0887 ,  B01L2300/12 ,  G01N15/1056 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/645 ,  G01N21/6486 ,  G01N2015/1006 ,  G01N2021/6482

Abstract: A microfluidic system-on-a-chip includes signal processing, light generation and detection, and fluid handling functions formed on a single substrate. The disclosed integrated system has a smaller footprint than device structures where individual components are manufactured separately and then assembled. Moreover, the integrated system obviates alignment challenges associated with conventionally packaged architecture.

公开(公告)号：US20170089881A1

公开(公告)日：2017-03-30

申请号：US15278581

申请日：2016-09-28

Applicant: The Board of Trustees of the University of Illinois

Inventor： Gaurav Bahl ,  Kewen Han

IPC: G01N33/487 ,  G01N29/02 ,  G01N29/24 ,  G01N15/14 ,  G01N21/17

CPC classification number: G01N33/487 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1436 ,  G01N15/1459 ,  G01N29/036 ,  G01N29/12 ,  G01N29/222 ,  G01N29/2418 ,  G01N2015/0065 ,  G01N2015/1043 ,  G01N2021/638 ,  G01N2291/02466 ,  G01N2291/02827

Abstract: A system and method includes resonator device to detect cells or other particles through light and/or vibration sensing.