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    Methods, compositions, and automated systems for separating rare cells from fluid samples
    1.
    发明授权
    Methods, compositions, and automated systems for separating rare cells from fluid samples 有权
    用于从流体样品中分离稀有细胞的方法，组合物和自动化系统

    公开(公告)号：US07166443B2

    公开(公告)日：2007-01-23

    申请号：US10701684

    申请日：2003-11-04

    申请人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    发明人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    IPC分类号： C12Q1/04

    CPC分类号： B01L3/502761 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  B01D57/02 ,  B01D61/18 ,  B01D67/0034 ,  B01D67/0062 ,  B01D71/02 ,  B01D71/027 ,  B01D71/04 ,  B01D2325/021 ,  B01D2325/04 ,  B01L3/502 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0644 ,  B01L2400/065 ,  G01N1/40 ,  G01N15/0272 ,  G01N15/05 ,  G01N2001/4016 ,  G01N2015/0073 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681

    摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients. The invention includes microfabricated filters for filtering fluid samples and methods of enriching rare cells of fluid samples using microfabricated filters of the present invention. The invention also includes solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample and methods of using selective RBC sedimentation solutions for enriching rare cells of a fluid sample. Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that includes a microfabricated filter; automated means for directing fluid flow through at least one filtration chamber of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using automated systems for separating rare cells from fluid samples. Preferred fluid samples are blood, effusion, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.

    摘要翻译： 本发明认识到许多病症的诊断和预后可以取决于来自复杂流体样品的稀有细胞的富集。 特别地，母体样品（例如母体血液样品）富集胎儿细胞可以大大有助于检测胎儿异常或各种遗传病症。 此外，本发明认识到，来自患者样品的稀有恶性细胞的富集可以帮助患者的治疗方式的诊断，预后和发展。 本发明包括用于过滤液体样品的微制造过滤器和使用本发明的微加工过滤器来富集稀有细胞液体样品的方法。 本发明还包括从血液样品中选择性沉降红细胞（RBC）的方案和使用选择性RBC沉降溶液富集流体样品稀有细胞的方法。 本发明的另一方面是一种用于处理流体样品的自动化系统，该系统包括：至少一个包括微加工过滤器的过滤室; 用于引导流体流过自动化系统的至少一个过滤室的自动装置，以及用于收集富集的稀有细胞的装置。 本发明还包括使用自动化系统从流体样品中分离稀有细胞的方法。 优选的流体样品是血液，渗出物或尿液样品，并且可以从这种样品中富集的稀有细胞包括成核红细胞和癌细胞。

    Methods, compositions, and automated systems for separating rare cells from fluid samples
    4.
    发明授权
    Methods, compositions, and automated systems for separating rare cells from fluid samples 有权

    公开(公告)号：US06949355B2

    公开(公告)日：2005-09-27

    申请号：US10268312

    申请日：2002-10-10

    申请人： Douglas Yamanishi ,  Paul Hujsak ,  Zhaohuai Yang ,  J{dot over (u)}nquan Xu ,  Xiaobo Wang ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Jing Cheng

    发明人： Douglas Yamanishi ,  Paul Hujsak ,  Zhaohuai Yang ,  J{dot over (u)}nquan Xu ,  Xiaobo Wang ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Jing Cheng

    IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/06 ,  C12N5/08 ,  G01N1/40 ,  G01N15/02 ,  G01N15/05 ,  G01N15/06 ,  C12Q1/04

    CPC分类号： G01N15/05 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  G01N1/40 ,  G01N15/0272 ,  G01N33/491 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681

    摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients.A first aspect of the present invention is a microfabricated filter for filtering a fluid sample. A microfabricated filter of the present invention comprises at least one tapered pore, and preferably comprises at least two tapered pores whose variation in size is 20% or less. The present invention also includes a method of enriching rare cells of a fluid sample using a microfabricated filter of the present invention.Another aspect of the invention is solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample comprising a red blood cell aggregating agent and at least one specific binding member that selectively binds RBCs. Solutions of the present invention include a combined solution for rare cell enrichment that comprise RBC aggregating agents, at least one specific binding member that selectively binds RBCs, and at least one additional specific binding member for the removal of undesirable sample components other than RBCs. The invention also includes methods of using selective RBC sedimentation solutions and combined solutions for enriching rare cells of a fluid sample.Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that comprises or engages one or more microfabricated filters of the present invention; automated means for directing fluid flow through the one or more filtration chambers of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using an automated system for separating rare cells from a fluid sample. Preferred fluid samples are effusion, blood, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.