公开(公告)号：US20050241941A1

公开(公告)日：2005-11-03

申请号：US11176805

申请日：2005-07-06

申请人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

发明人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

IPC分类号： G01N31/20 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/25 ,  G01N27/26 ,  G01N27/27 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/574 ,  G01N35/08 ,  G01N37/00

CPC分类号： G01N33/502 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5064 ,  G01N33/5091 ,  G01N33/5097 ,  G01N33/5304 ,  G01N33/57415 ,  G01N35/08 ,  Y10S366/02 ,  Y10S435/81 ,  Y10S435/817 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/25

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.

摘要翻译： 本发明提供了可用于进行高通量筛选测定的新型微流体装置和方法。 特别地，本发明的装置和方法可用于筛选大量不同的化合物，因为它们对多种化学品，优选生物化学体系的影响。

公开(公告)号：US20060000722A1

公开(公告)日：2006-01-05

申请号：US11050900

申请日：2005-02-03

申请人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

发明人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

IPC分类号： G01N27/26

CPC分类号： C12Q1/00 ,  B01J19/0093 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.

公开(公告)号：US20060275817A1

公开(公告)日：2006-12-07

申请号：US11460550

申请日：2006-08-11

申请人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

发明人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US20060258019A1

公开(公告)日：2006-11-16

申请号：US11461163

申请日：2006-07-31

申请人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

发明人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

IPC分类号： B01L3/00

CPC分类号： B01D61/18 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  Y10T137/2496 ,  Y10T137/2499 ,  Y10T137/2501 ,  Y10T137/2514 ,  Y10T137/2516 ,  Y10T436/11 ,  Y10T436/2575

摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds that are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.

摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供制造歧管的方法。

公开(公告)号：US20070157973A1

公开(公告)日：2007-07-12

申请号：US11702368

申请日：2007-02-03

申请人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

IPC分类号： F15C1/04 ,  F04B41/06

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

公开(公告)号：US20050005684A1

公开(公告)日：2005-01-13

申请号：US10916270

申请日：2004-08-11

申请人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

IPC分类号： G01N11/04 ,  B01L3/00 ,  B01L9/00 ,  G01N11/08 ,  G01N27/06 ,  G01N27/447 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00 ,  G05D7/06 ,  G01N27/26

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

摘要翻译： 改进的微流体装置，系统和方法允许通过在多个储存器处施加，控制和改变压力来选择性地输送微流体网络的微流体通道内的流体。 将微流体网络建模为通过通道段连接在一起的一系列节点并且确定通道段的流动阻力特性可以允许计算通过在储存器处的给定压力构造导致的通道段的流体流动。 为了在特定通道或一系列通道内实现期望的流量，可以使用网络模型来识别储层压力。 粘度计或其他流量传感器可以测量通道内的流量特性，并且可以使用测量的流量特性来计算产生期望流量的压力。 多储层压力调节器和压力控制器系统可以可选地与电动或其它流体输送机构结合使用。

公开(公告)号：US20080085521A1

公开(公告)日：2008-04-10

申请号：US11867626

申请日：2007-10-04

申请人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

发明人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  B01L3/5027 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2545/113 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143

摘要： Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.

摘要翻译： 提供了用于进行单分子扩增用于核酸检测，定量和统计分析的方法和系统。 提供了用于确定和定量感兴趣的核酸的长度的方法和系统。

公开(公告)号：US20070154895A1

公开(公告)日：2007-07-05

申请号：US11323992

申请日：2005-12-30

申请人： Michael Spaid ,  Anne Kopf-Sill ,  Cheryl Cathey

发明人： Michael Spaid ,  Anne Kopf-Sill ,  Cheryl Cathey

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12P19/34 ,  C12M1/34

CPC分类号： G01N33/54366 ,  B01L3/0293 ,  B01L3/502715 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0487 ,  G01N2035/00158

摘要： This invention provides systems and methods of running multiple different analyses on a microfluidic device. Results from such analyses can be useful in high throughput screening of samples or correlation of multiple as

摘要翻译： 本发明提供了在微流体装置上运行多个不同分析的系统和方法。 这种分析的结果可用于样品的高通量筛选或多重相关性

公开(公告)号：US07171983B2

公开(公告)日：2007-02-06

申请号：US10916270

申请日：2004-08-11

申请人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

IPC分类号： F15C1/04 ,  F04B41/06

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

摘要翻译： 改进的微流体装置，系统和方法允许通过在多个储存器处施加，控制和改变压力来选择性地输送微流体网络的微流体通道内的流体。 将微流体网络建模为通过通道段连接在一起的一系列节点并且确定通道段的流动阻力特性可以允许计算通过在储存器处的给定压力构造导致的通道段的流体流动。 为了在特定通道或一系列通道内实现期望的流量，可以使用网络模型来识别储层压力。 粘度计或其他流量传感器可以测量通道内的流量特性，并且可以使用测量的流量特性来计算产生期望流量的压力。 多储层压力调节器和压力控制器系统可以可选地与电动或其它流体输送机构结合使用。

公开(公告)号：US06915679B2

公开(公告)日：2005-07-12

申请号：US09792435

申请日：2001-02-23

申请人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

IPC分类号： G01N11/04 ,  B01L3/00 ,  B01L9/00 ,  G01N11/08 ,  G01N27/06 ,  G01N27/447 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00 ,  G05D7/06 ,  G01N11/00

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.