公开(公告)号：US20070075010A1

公开(公告)日：2007-04-05

申请号：US11479125

申请日：2006-06-30

申请人： John Gilbert ,  Manish Deshpande ,  Jaishree Trikha

发明人： John Gilbert ,  Manish Deshpande ,  Jaishree Trikha

IPC分类号： B01D63/00 ,  G01N33/00 ,  C02F1/40

CPC分类号： B01D63/087 ,  B01D63/088 ,  B01D67/0034 ,  B01D67/0062 ,  B01D2313/13 ,  B01D2313/40 ,  B01D2325/028 ,  B01D2325/12 ,  B01L3/06 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/146 ,  B01L2300/041 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0421 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0666 ,  C02F2209/36 ,  C30B7/00 ,  C30B29/58 ,  G01N27/44743 ,  G01N27/44773 ,  Y10S977/786 ,  Y10T156/10

摘要： A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.

摘要翻译： 用于在微流体系统中整合微流体组分的系统和方法使得微流体系统能够执行选定的微流体功能。 封盖模块包括用于执行微流控功能的微流体元件。 封盖模块堆叠在具有微流管道的微流体衬底上，以将微流控功能结合到系统中。 微流体元件可以包含对样品中选定分子具有亲和性的基质。 该基质与所选择的分子结合，反应和/或保留，而不影响样品中的其它分子。

公开(公告)号：US20060269446A1

公开(公告)日：2006-11-30

申请号：US11295183

申请日：2005-12-05

申请人： John Gilbert ,  Hugh Lewis ,  Derek Beaupre ,  Jaishree Trikha ,  Manish Deshpande

发明人： John Gilbert ,  Hugh Lewis ,  Derek Beaupre ,  Jaishree Trikha ,  Manish Deshpande

IPC分类号： B01L9/00

CPC分类号： G01N33/577 ,  B01L3/502761 ,  B01L3/502776 ,  B01L5/00 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/082 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/1056 ,  G01N15/1484 ,  G01N2015/1486 ,  G01N2015/149 ,  Y10T436/2575

摘要： A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

摘要翻译： 用于在颗粒上进行过程的单个一次性盒，例如颗粒分选，将所有流体接触表面封装在盒中以用于微流体颗粒处理技术。 墨盒与操作系统接口，用于进行颗粒处理。 流体接触表面的封装确保，改善或促进操作者隔离和/或产品隔离。 墨盒可以采用任何合适的处理颗粒的技术。

公开(公告)号：US07153699B2

公开(公告)日：2006-12-26

申请号：US10328932

申请日：2002-12-23

申请人： John Gilbert ,  Jaishree Trikha

发明人： John Gilbert ,  Jaishree Trikha

IPC分类号： G01N1/10 ,  G01N1/00 ,  G01N1/40 ,  B01L3/02

CPC分类号： C40B50/08 ,  B01L3/0244 ,  B01L3/0248 ,  B01L3/0255 ,  B01L3/06 ,  B01L3/5088 ,  B01L2200/142 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2400/0439 ,  C40B40/10 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

摘要： A two-pin liquid handling system for crystallizing biomolecules comprises a pair of interacting pins for holding a droplet of liquid therebetween. Each pin includes a tip spaced predetermined distance from the other pin to define a sample acquisition region. The two-pin liquid handling system includes a temperature and humidity control element for controlling the temperature of the sample acquisition region to cause transpiration of water to and from a droplet of biomolecule solution to promote crystallization of the biomolecule.

公开(公告)号：US20050112774A1

公开(公告)日：2005-05-26

申请号：US10973138

申请日：2004-10-25

申请人： John Gilbert ,  Jaishree Trikha

发明人： John Gilbert ,  Jaishree Trikha

IPC分类号： B01L3/00 ,  B01L3/06 ,  C30B7/00

CPC分类号： C30B7/00 ,  B01L3/0248 ,  B01L3/06 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502753 ,  B01L2200/0678 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0487 ,  C30B29/58 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/255

摘要： A system for crystallizing a protein and other biomolecules comprises a flow channel for conveying a solution containing the biomolecule to be crystallized and one or more substations related to the crystallization process formed along the flow channel. The system operates by flowing a biomolecule solution through a the flow channel, then performing dialysis on a micro-scale to set crystallization conditions. After dialysis, the concentration of the biomolecule solution is changed by removing water or solvent from the biomolecule solution to promote formation of crystals in a segment of the flow channel. Formed crystals may be observed and harvested from the flow channel.

摘要翻译： 用于结晶蛋白质和其他生物分子的系统包括用于输送含有待结晶的生物分子的溶液的流动通道和与沿流动通道形成的结晶过程相关的一个或多个变电站。 该系统通过使生物分子溶液流过流动通道，然后在微量级上进行透析以设定结晶条件来操作。 透析后，通过从生物分子溶液中除去水或溶剂来改变生物分子溶液的浓度，以促进在流动通道的段中形成晶体。 可以从流动通道观察并收获形成的晶体。

公开(公告)号：US20070054408A1

公开(公告)日：2007-03-08

申请号：US11595543

申请日：2006-11-10

申请人： John Gilbert ,  Jaishree Trikha

发明人： John Gilbert ,  Jaishree Trikha

IPC分类号： G01N33/00

CPC分类号： C30B29/58 ,  C30B7/00

摘要： A two-pin liquid handling system for crystallizing biomolecules comprises a pair of interacting pins for holding a droplet of liquid therebetween. Each pin includes a tip spaced predetermined distance from the other pin to define a sample acquisition region. The two-pin liquid handling system includes a temperature and humidity control element for controlling the temperature of the sample acquisition region to cause transpiration of water to and from a droplet of biomolecule solution to promote crystallization of the biomolecule.

摘要翻译： 用于结晶生物分子的双针液体处理系统包括一对用于在其间保持液滴的相互作用的销。 每个引脚包括与另一个引脚间隔预定距离的尖端以限定采样区域。 双针液体处理系统包括温度和湿度控制元件，用于控制样品采集区域的温度，以使水与生物分子溶液液滴一起蒸发以促进生物分子的结晶。

公开(公告)号：US06849459B2

公开(公告)日：2005-02-01

申请号：US10328931

申请日：2002-12-23

申请人： John Gilbert ,  Jaishree Trikha

发明人： John Gilbert ,  Jaishree Trikha

IPC分类号： B01L3/00 ,  B01L3/06 ,  C30B7/00 ,  G01N33/00

CPC分类号： C30B7/00 ,  B01L3/0248 ,  B01L3/06 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502753 ,  B01L2200/0678 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0487 ,  C30B29/58 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/255

摘要： A system for crystallizing a protein and other biomolecules comprises a flow channel for conveying a solution containing the biomolecule to be crystallized and one or more substations related to the crystallization process formed along the flow channel. The system operates by flowing a biomolecule solution through a the flow channel, then performing dialysis on a micro-scale to set crystallization conditions. After dialysis, the concentration of the biomolecule solution is changed by removing water or solvent from the biomolecule solution to promote formation of crystals in a segment of the flow channel. Formed crystals may be observed and harvested from the flow channel.

摘要翻译： 用于结晶蛋白质和其他生物分子的系统包括用于输送含有待结晶的生物分子的溶液的流动通道和与沿流动通道形成的结晶过程相关的一个或多个变电站。 该系统通过使生物分子溶液流过流动通道，然后在微量级上进行透析以设定结晶条件来操作。 透析后，通过从生物分子溶液中除去水或溶剂来改变生物分子溶液的浓度，以促进在流动通道的段中形成晶体。 可以从流动通道观察并收获形成的晶体。

公开(公告)号：US20060278288A1

公开(公告)日：2006-12-14

申请号：US11433781

申请日：2006-05-12

申请人： John Gilbert ,  Sebastian Bohm ,  Manish Deshpande

发明人： John Gilbert ,  Sebastian Bohm ,  Manish Deshpande

IPC分类号： F15C1/04

CPC分类号： B01L3/502738 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/06 ,  B01L2400/0688 ,  B01L2400/082 ,  B07C5/34 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0034 ,  F16K99/0036 ,  F16K99/004 ,  F16K99/0046 ,  F16K99/0048 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2015/149 ,  Y10T29/49405 ,  Y10T137/0318 ,  Y10T137/0396 ,  Y10T137/0497 ,  Y10T137/2076 ,  Y10T137/2082 ,  Y10T137/2191 ,  Y10T137/2196 ,  Y10T137/2202 ,  Y10T137/2207 ,  Y10T137/2213 ,  Y10T137/2224 ,  Y10T436/143333 ,  Y10T436/25375

摘要： A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.

公开(公告)号：US20050123450A1

公开(公告)日：2005-06-09

申请号：US10979848

申请日：2004-11-01

申请人： John Gilbert ,  Manish Deshpande ,  Bernard Bunner

发明人： John Gilbert ,  Manish Deshpande ,  Bernard Bunner

IPC分类号： B01F20060101 ,  B01F13/00 ,  B01L3/00 ,  B32B5/02 ,  B65G51/00 ,  G01N15/14

CPC分类号： B65G51/08 ,  B01F13/0062 ,  B01F13/0064 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00891 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B65G51/00 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1409

摘要： A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

摘要翻译： 用于产生鞘流的微细鞘层流动结构包括用于输送护套流体的初级鞘流动通道，用于将样品注入主鞘流道中的鞘液中的样品入口，用于将样品聚焦在 护套流体和辅助聚焦区域，用于提供样品在护套液体内的附加聚焦。 次要聚焦区域可以由与主鞘流道相交的流动通道形成，以从选定的方向将另外的鞘流体注入主鞘流动通道。 护套流动系统可以包括在微流体芯片上并行操作的多个护套流动结构。

公开(公告)号：US20050109410A1

公开(公告)日：2005-05-26

申请号：US11021251

申请日：2004-12-21

申请人： John Gilbert ,  Sebastian Bohm ,  Manish Deshpande

发明人： John Gilbert ,  Sebastian Bohm ,  Manish Deshpande

IPC分类号： B01L3/00 ,  B07C5/34 ,  F15C5/00 ,  F16K99/00 ,  G01N15/14 ,  F15C1/04

CPC分类号： B01L3/502738 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/06 ,  B01L2400/0688 ,  B01L2400/082 ,  B07C5/34 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0034 ,  F16K99/0036 ,  F16K99/004 ,  F16K99/0046 ,  F16K99/0048 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2015/149 ,  Y10T29/49405 ,  Y10T137/0318 ,  Y10T137/0396 ,  Y10T137/0497 ,  Y10T137/2076 ,  Y10T137/2082 ,  Y10T137/2191 ,  Y10T137/2196 ,  Y10T137/2202 ,  Y10T137/2207 ,  Y10T137/2213 ,  Y10T137/2224 ,  Y10T436/143333 ,  Y10T436/25375

摘要： A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.

公开(公告)号：US08567608B2

公开(公告)日：2013-10-29

申请号：US12499254

申请日：2009-07-08

申请人： Manish Deshpande ,  John Gilbert

发明人： Manish Deshpande ,  John Gilbert

IPC分类号： B07C5/00

CPC分类号： B07C5/36 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0487 ,  B07C5/02 ,  B07C5/34 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0028 ,  F16K99/0046 ,  F16K99/0048 ,  G01N15/10 ,  G01N15/14 ,  G01N2015/1075 ,  G01N2015/1081 ,  G01N2015/1477 ,  G01N2015/149 ,  G01N2015/1497 ,  Y10S209/906 ,  Y10S209/932

摘要： A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.