公开(公告)号：US20060292611A1

公开(公告)日：2006-12-28

申请号：US11448462

申请日：2006-06-06

申请人： Jan Berka ,  Zhoutao Chen ,  Michael Egholm ,  Brian Godwin ,  Stephen Hutchison ,  John Leamon ,  Gary Sarkis ,  Jan Simons

发明人： Jan Berka ,  Zhoutao Chen ,  Michael Egholm ,  Brian Godwin ,  Stephen Hutchison ,  John Leamon ,  Gary Sarkis ,  Jan Simons

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q2537/155 ,  C12Q2525/191 ,  C12Q2521/301 ,  C12Q2533/107 ,  C12Q2531/125 ,  C12Q2521/319 ,  C12Q2521/125 ,  C12Q2565/518 ,  C12Q2563/131

摘要： The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

公开(公告)号：US20050079510A1

公开(公告)日：2005-04-14

申请号：US10767899

申请日：2004-01-28

申请人： Jan Berka ,  Yi-Ju Chen ,  John Leamon ,  Steve Lefkowitz ,  Kenton Lohman ,  Vinod Makhijani ,  Jonathan Rothberg ,  Gary Sarkis ,  Maithreyan Srinivasan ,  Michael Weiner

发明人： Jan Berka ,  Yi-Ju Chen ,  John Leamon ,  Steve Lefkowitz ,  Kenton Lohman ,  Vinod Makhijani ,  Jonathan Rothberg ,  Gary Sarkis ,  Maithreyan Srinivasan ,  Michael Weiner

IPC分类号： B01L3/00 ,  C07H21/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/25 ,  G01N21/64

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。

公开(公告)号：US20060228721A1

公开(公告)日：2006-10-12

申请号：US11104781

申请日：2005-04-12

申请人： John Leamon ,  William Lee ,  Jan Simons ,  Brian Desany ,  Michael Ronan ,  James Drake ,  Kenton Lohman ,  Michael Egholm ,  Jonathan Rothberg

发明人： John Leamon ,  William Lee ,  Jan Simons ,  Brian Desany ,  Michael Ronan ,  James Drake ,  Kenton Lohman ,  Michael Egholm ,  Jonathan Rothberg

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6858 ,  C12Q2565/301 ,  C12Q2531/107 ,  C12Q2531/101 ,  C12Q2565/537 ,  C12Q2565/515 ,  C12Q2545/114

摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.

摘要翻译： 所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。 本公开中提供的技术允许在一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。 结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。

公开(公告)号：US08728728B2

公开(公告)日：2014-05-20

申请号：US13619815

申请日：2012-09-14

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US20100304982A1

公开(公告)日：2010-12-02

申请号：US12785685

申请日：2010-05-24

申请人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan M. Rothberg

发明人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan M. Rothberg

IPC分类号： C40B30/00 ,  C40B40/08 ,  C40B50/18 ,  C12P19/34

CPC分类号： B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US08978939B2

公开(公告)日：2015-03-17

申请号：US11573715

申请日：2005-08-09

申请人： Martin C. Bull ,  John Leamon

发明人： Martin C. Bull ,  John Leamon

IPC分类号： B65D5/72 ,  B65D47/20

CPC分类号： B65D47/2031 ,  B65D2547/066

摘要： A retaining device (1) has a non-laminar self-closing valve (2) retained therein. The valve comprises a flange (8), and the rigidity of the flange (8) is increased by the device (1). The retaining device is a single-piece article.

摘要翻译： 保持装置（1）具有保持在其中的非层流自闭阀（2）。 该阀包括凸缘（8），凸缘（8）的刚度由装置（1）增加。 保持装置是单件制品。

公开(公告)号：US08728736B2

公开(公告)日：2014-05-20

申请号：US13619178

申请日：2012-09-14

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

摘要翻译： 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许一个或多个靶序列的选择性扩增的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变体的存在。

公开(公告)号：US08673560B2

公开(公告)日：2014-03-18

申请号：US13618805

申请日：2012-09-14

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US08470164B2

公开(公告)日：2013-06-25

申请号：US13001182

申请日：2009-06-25

申请人： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

发明人： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC分类号： G01N27/414 ,  C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US20110201523A1

公开(公告)日：2011-08-18

申请号：US13027336

申请日：2011-02-15

申请人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： C40B40/06

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中颗粒对颗粒的变化减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。