摘要:
A method for producing an apparatus for transferring small amounts of liquids includes bonding a plurality of parallel fibers having plural coaxial layers into a bundle, slicing the bundle of parallel fibers in planes perpendicular to the direction of the fibers to form two opposite, planar surfaces, and selectively etching the fiber layers to create etched wells in the fibers at one of the planar surfaces. The etched wells are in fluid communication with corresponding capillary nozzles of the fibers that extend to an opposite one of the planar surfaces. Various apparatus configurations of the present invention include liquid transfer devices manufactured utilizing one or more of the various method configurations of the present invention. By way of example only, a bundle of three-layer optical fibers or a bundle of hollow two-layer optical fibers may be utilized to produce a liquid transfer device.
摘要:
The present inventions provides an apparatus and method for the parallel loading of multiple samples in a microarray containing a plurality of sub-arrays. The method makes use of a microarray containing multiple sub-arrays, a loading channel array, and a fluid handling robot or an assembly robot or machine.
摘要:
Embodiments of the invention are directed to microfluidic devices. In one embodiment, a microanalysis chip comprises a body having at least one transfer-separation channel with a channel bottom that has a bottom opening. The transfer-separation channel terminates in a discharge aperture.
摘要:
A method and apparatus for analyzing molecular structures within a sample substance using an array having a plurality of test sites upon which the sample substance is applied. The invention is also directed to a method and apparatus for constructing molecular arrays having a plurality of test sites. The invention allows for definitive high throughput analysis of multiple analytes in complex mixtures of sample substances. A combinatorial analysis process is described that results in the creation of an array of integrated chemical devices. These devices operate in parallel, each unit providing specific sets of data that, when taken as a whole, give a complete answer for a defined experiment. This approach is uniquely capable of rapidly providing a high density of information from limited amounts of sample in a cost-effective manner.
摘要:
Microarray is produced by the method comprising the steps of forming a droplet of a liquid sample containing a biological substance at a pouring port of a micropipette by ejecting a predetermined amount of the liquid sample from the micropipette, supporting the micropipette at a position where the droplet formed at the pouring port can contact with a substrate, and transferring the droplet formed at the pouring port to the substrate, thereby spotting the sample on the substrate. The microarray has a plurality of visually identifiable spots of samples on the substrate.
摘要:
There is provided according to the invention an apparatus for liquid sample handling which comprises: (a) a plurality of hollow capillaries, each being open at both ends and of defined internal volume, wherein on contact of an open end of a capillary with a sample, said defined volume of sample is drawn up into the capillary by capillary action; (b) a housing which retains the capillaries in their desired orientation; and (c) means to effect sample removal from the capillaries; and processes employing said apparatus.
摘要:
The present invention provides instruments and methods for screening combinatorial libraries that addresses many of the problems encountered when using conventional instruments. For example, the disclosed instruments can measure mechanical properties of library members in rapid serial or parallel test format, and can perform tests on small amounts of material, which are easily prepared or dispensed using art-disclosed liquid or solid handling techniques. Compared to conventional instruments, the disclosed instruments afford faster sample loading and unloading, for example, through the use of disposable libraries of material samples.
摘要:
The present invention provides a method and apparatus for dispensing a small volume of a selected liquid, such as a biological sample or reagent, onto a substrate. The device includes a tube adapted to contain the liquid. An elongate fiber is disposed within the tube for axial movement therein between raised and lowered positions. Upon shifting or oscillating the fiber between its raised and lowered positions, a liquid spot can be formed at a selected position on the substrate. The device is readily adaptable for the production of micro-arrays having a great number of individual spots.
摘要:
The invention features methods of making devices, or nullplatensnull, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.
摘要:
Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.