公开(公告)号：US12038438B2

公开(公告)日：2024-07-16

申请号：US17509933

申请日：2021-10-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  G01N33/542 ,  G01N33/58 ,  B01L7/00 ,  G01N21/64

CPC classification number: G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2500/00

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20240229130A9

公开(公告)日：2024-07-11

申请号：US18381400

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

公开(公告)号：US12030051B2

公开(公告)日：2024-07-09

申请号：US16419485

申请日：2019-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Austin Payne ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Yi Zhou ,  Saedeh Javdani Sepehri

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L3/5635 ,  B01L7/00 ,  G01N35/0098 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.

公开(公告)号：US11982870B2

公开(公告)日：2024-05-14

申请号：US17811574

申请日：2022-07-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Rodney C. Harris

IPC: G02B7/182 ,  G02B26/08 ,  G02B27/14

CPC classification number: G02B7/1821 ,  G02B7/1825 ,  G02B26/0816 ,  G02B27/141

Abstract: Disclosed is an adjustable mirror mount that is capable of adjusting a mirror in two axes with a high degree of precision and low cross-coupling. Long horizontal and vertical adjustment arms are used to allow the precision adjustment about both a horizontal axis and a vertical axis.

公开(公告)号：US11980883B2

公开(公告)日：2024-05-14

申请号：US16641676

申请日：2018-08-22

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： William Strong ,  Daniel Y. Chu

IPC: B01L9/00 ,  B01L3/00

CPC classification number: B01L3/5023 ,  B01L2200/12 ,  B01L2300/0609 ,  B01L2300/0825 ,  B01L2400/0406

Abstract: Lateral flow device pump housings and methods of making such housings are provided.

公开(公告)号：US11965891B2

公开(公告)日：2024-04-23

申请号：US15393445

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe Gmbh

Inventor： Jeremy Agresti ,  Ronald Lebofsky

IPC: G01N33/68 ,  B01J19/00 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00

CPC classification number: G01N33/6878 ,  B01J19/0046 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00 ,  C12Q1/6816 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q1/6869 ,  C12Q2565/514

Abstract: Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.

公开(公告)号：US20240124856A1

公开(公告)日：2024-04-18

申请号：US18449107

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US11946855B2

公开(公告)日：2024-04-02

申请号：US18195084

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10 ,  G01N33/487 ,  G06V20/69 ,  G01N15/02 ,  G01N35/00

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G06V20/69

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11939573B2

公开(公告)日：2024-03-26

申请号：US16935754

申请日：2020-07-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge Saxonov

IPC: C12N15/10 ,  C12Q1/6876

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q2525/191 ,  C12Q2563/179

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20240093271A1

公开(公告)日：2024-03-21

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.