公开(公告)号：US12070746B2

公开(公告)日：2024-08-27

申请号：US15958142

申请日：2018-04-20

申请人： EMULATE,INC. ,  CEDARS-SINAI MEDICAL CENTER

发明人： Dan Gazit ,  Gadi Pelled ,  Zulma Gazit ,  Dmitriy Sheyn ,  Christopher David Hinojosa ,  Norman Wen ,  Geraldine Hamilton

IPC分类号： C12N5/077 ,  A61K49/00 ,  A61K51/02 ,  A61L27/38 ,  B01L3/00 ,  C12N5/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/0775 ,  G01N33/50 ,  G01T1/164 ,  G01T1/29 ,  C07K14/78 ,  C12M1/34

CPC分类号： B01L3/5027 ,  A61K49/0019 ,  A61K49/005 ,  A61K51/02 ,  A61L27/3895 ,  B01L3/5085 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0668 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5038 ,  G01T1/1644 ,  G01T1/2985 ,  A61M2205/3334 ,  C07K14/78 ,  C12M41/40

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US12066440B2

公开(公告)日：2024-08-20

申请号：US17082979

申请日：2020-10-28

申请人： Labrador Diagnostics LLC

发明人： Chinmay Pangarkar ,  Karan Mohan ,  James R. Wasson

IPC分类号： B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C08L5/08 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  G01N1/30 ,  G01N15/10 ,  G01N21/03 ,  G01N21/05 ,  G01N21/17 ,  G01N21/27 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/53 ,  G01N33/564 ,  G01N33/569 ,  G01N33/58 ,  G01N33/68 ,  G02B7/09 ,  G02B21/00 ,  G02B21/08 ,  G02B21/12 ,  G02B21/16 ,  G02B21/24 ,  G02B21/36

CPC分类号： G01N33/56972 ,  B01L3/502715 ,  G01N1/30 ,  G01N15/1012 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/17 ,  G01N21/27 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/487 ,  G01N33/49 ,  G01N33/5005 ,  G01N33/53 ,  G01N33/5308 ,  G01N33/56966 ,  G01N33/582 ,  G02B7/09 ,  G02B21/0076 ,  G02B21/088 ,  G02B21/125 ,  G02B21/16 ,  G02B21/244 ,  G02B21/365 ,  G01N2015/1014 ,  G01N2021/1738 ,  G01N2021/6439 ,  G01N2201/061 ,  G01N2201/12 ,  G01N2333/70589 ,  G01N2333/70596

摘要： Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

公开(公告)号：US12065675B2

公开(公告)日：2024-08-20

申请号：US17464024

申请日：2021-09-01

申请人： OLYMPUS CORPORATION

发明人： Ryutaro Akiyoshi

IPC分类号： C12N5/074 ,  C12M1/34 ,  C12M1/42 ,  C12N15/85 ,  C12Q1/66 ,  G01N21/64

CPC分类号： C12N5/0696 ,  C12M35/02 ,  C12M41/46 ,  C12N15/85 ,  C12Q1/66 ,  G01N21/6428 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2800/108 ,  G01N2021/6439

摘要： A selection method of an iPS cell includes: at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell; judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; and when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.

公开(公告)号：US12065636B2

公开(公告)日：2024-08-20

申请号：US16372106

申请日：2019-04-01

申请人： INV NYLON CHEMICALS AMERICAS, LLC

发明人： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12P7/625

CPC分类号： C12M29/24 ,  C12M41/32 ,  C12M41/34 ,  C12P7/625

摘要： Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

公开(公告)号：US20240271119A1

公开(公告)日：2024-08-15

申请号：US18292421

申请日：2022-07-27

申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College

发明人： David R. Liu ,  Tina Wang ,  Mary S. Morrison

IPC分类号： C12N15/10 ,  C12M1/00 ,  C12M1/34 ,  C12N1/20 ,  C12N7/00 ,  C12N15/70 ,  C12R1/19

CPC分类号： C12N15/1024 ,  C12M23/58 ,  C12M29/18 ,  C12M41/36 ,  C12N1/205 ,  C12N7/00 ,  C12N15/1058 ,  C12N15/70 ,  C12R2001/19

摘要： Aspects of the disclosure relate to compositions, systems, and methods for evolving nucleic acids and proteins utilizing continuous directed evolution in the periplasm of a host cell. In some embodiments, the methods comprise passing a nucleic acid from cell-to-cell in a desired, function dependent manner. The linkage of the desired function and passage of the nucleic acid from cell-to-cell allows for continuous selection and mutation of the nucleic acid.

公开(公告)号：US20240271078A1

公开(公告)日：2024-08-15

申请号：US18567750

申请日：2022-06-06

申请人： AMGEN INC.

发明人： Jeremy S. Conner ,  Sarah Whetstone ,  Ketan Kumar

IPC分类号： C12M1/36 ,  C12M1/34

CPC分类号： C12M41/48 ,  C12M41/36 ,  C12M41/46

摘要： A method of cell culture assessment (e.g., prior to a drug substance harvesting process) includes obtaining a plurality of parameters associated with a cell culture, and inferring or predicting a value or classification indicative of packed cell volume. Inferring or predicting the packed cell volume includes applying the plurality of parameters as inputs to a non-linear machine learning model. The method also includes generating an output indicative of the inferred or predicted value or classification.

公开(公告)号：US20240271066A1

公开(公告)日：2024-08-15

申请号：US18570211

申请日：2022-06-29

申请人： RAMOT AT TEL AVIV UNIVERSITY LTD.

发明人： Ben Meir MAOZ ,  Anna HERLAND ,  Shay DIVALD ,  Yuval OLIEL

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/06

CPC分类号： C12M21/08 ,  C12M23/16 ,  C12M23/34 ,  C12M23/42 ,  C12M25/04 ,  C12M29/06 ,  C12M41/46

摘要： Provided herein is a modular organ-on-a-chip platform, configured for cultivating cells, tissues, organoids and organelles and capable of performing high throughput analyses, including pharmacokinetic evaluations.

公开(公告)号：US12061150B2

公开(公告)日：2024-08-13

申请号：US17059690

申请日：2019-05-31

申请人： ORB XYZ, INC.

发明人： Lorenzo Falzarano ,  Andreia Michelle Smith-Moritz

IPC分类号： B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N21/6486 ,  C12Q1/04 ,  G01N33/1826

摘要： The invention generally relates to detecting biological substances. In certain aspects, the invention is directed to a method directing one or more wavelengths of light within a deep ultraviolet (UV) spectrum into a medium to excite a biological substance in the medium, detect emission from the excited biological substance via a plurality of semiconductor photodetectors, and analyze the deep UV emission data for presence of a deep UV spectral signature indicative of the biological substance, wherein presence of the deep UV spectral signature indicates that the medium comprises a biological substance. The invention is also directed to identifying a pathogen in a medium comprising a pathogen and a non-pathogen biological substance.

公开(公告)号：US12060549B2

公开(公告)日：2024-08-13

申请号：US17156275

申请日：2021-01-22

申请人： UTS BIOGASTECHNIK GMBH

发明人： Andreas Czwaluk

IPC分类号： C12M1/06 ,  B01F27/25 ,  B01F27/704 ,  B01F27/705 ,  B01F27/706 ,  B01F33/81 ,  B01F35/212 ,  B01F35/22 ,  B01F35/221 ,  C12M1/107 ,  C12M1/34 ,  C12M1/36 ,  C12P1/00 ,  B01F101/44

CPC分类号： C12M27/02 ,  B01F27/25 ,  B01F27/704 ,  B01F27/705 ,  B01F27/706 ,  B01F33/81 ,  B01F35/212 ,  B01F35/2202 ,  B01F35/221422 ,  C12M21/04 ,  C12M41/42 ,  C12M41/48 ,  C12P1/00 ,  B01F2101/44 ,  Y02E50/30

摘要： A method for operating an agitating device and a digester, wherein the digester is filled with a substrate and an agitating device is in the digester. These steps are performed: a) A target load curve is lodged in the control device; b) the control device prescribes a target rotation speed; c) the control device operates the agitating device at an actual speed corresponding to a target speed; d) the control device captures an actual agitating device torque measurement value at the actual speed of rotation; e) the control device derives from the actual measurement value an actual characteristic value of the agitating device applied torque; f) the control device compares the derived actual characteristic value against the target characteristic value of the substrate resulting from the target load curve at the prescribed target speed; and g) the control device controls the agitating device in dependence on the result of comparison.

公开(公告)号：US20240254428A1

公开(公告)日：2024-08-01

申请号：US18564036

申请日：2022-05-27

申请人： Cody Gilleland, Ph.D.

发明人： Cody Gilleland, Ph.D.

IPC分类号： C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  C12N15/89 ,  G16B40/10

CPC分类号： C12M41/48 ,  C12M41/06 ,  C12M41/14 ,  C12M41/36 ,  C12N15/89 ,  G16B40/10

摘要： In the present invention the robotic microinjection platform construction is provided in which three cameras enable simultaneous video streaming of high magnification, low magnification camera views with a third horizon view for Z-depth information to enable microneedle alignment. The triple camera method in combination with image processing algorithms enables automated microneedle alignment and organism targeting in low magnification followed by microneedle manipulations of the organism in high magnification. The object detection algorithms are trained using neural networks on large image datasets. The object detection algorithms improve over time as the training dataset grows as data is acquired with more experimentation.