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    Beads alignment structure, production method thereof, and bead alignment method for capillary beads array
    56.
    发明授权
    Beads alignment structure, production method thereof, and bead alignment method for capillary beads array 有权
    珠粒对准结构，其制备方法和毛细管珠阵列的珠粒对准方法

    公开(公告)号：US07052570B2

    公开(公告)日：2006-05-30

    申请号：US10765075

    申请日：2004-01-28

    申请人： Osamu Kogi ,  Hiroshi Kishida

    发明人： Osamu Kogi ,  Hiroshi Kishida

    IPC分类号： B29C37/00

    CPC分类号： B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0668 ,  B82Y30/00 ,  C40B40/06 ,  Y10T428/2982

    摘要： A method for producing a capillary bead array comprises the steps of: dispensing beads into a liquid pool, outside a capillary, having a depth of almost the same length as the particle diameter of a bead; leveling the excessive beads by moving a leveling member which is in contact with and relatively capable of be moved to the liquid pool to remove excessive beads that the liquid pool cannot contain; aligning the beads in the liquid pool one- or two-dimensionally; bonding adjacent individual beads to each other; producing a structure having the plurality of beads bonded and aligned one- or two-dimensionally; removing the structure from the liquid pool; and disposing the structure in the capillary formed of soft resin, so that the beads comprising the plurality of beads retaining the one- or two-dimensional alignment can be introduced simultaneously into the capillary. This method reduces the time and cost necessary for bead array production and retains the bead alignment in the capillary, resulting in improved accuracy and reliability in experiments using the capillary array.

    摘要翻译： 用于制备毛细管珠阵列的方法包括以下步骤：将珠子分散到具有与珠的粒径几乎相同长度的深度的毛细管外的液池中; 通过移动与液池接触并相对能够移动到液池的调平构件来调平多余的珠子，以去除液池不能容纳的多余的珠子; 一次或二维地将液体池中的珠子对准; 将相邻的单个珠彼此粘合; 产生具有多个珠粒的结构，其一次或二维地结合并排列; 从液池中取出结构; 并将结构设置在由软树脂形成的毛细管中，使得包含保持一维或二维取向的多个珠的珠可以同时引入毛细管中。 该方法减少了珠阵列生产所需的时间和成本，并且保持毛细管中的珠粒对准，从而在使用毛细管阵列的实验中提高了精度和可靠性。

    Substrate for biomolecule microarray, process for producing the same, biomolecule microarray and method of collecting data on biomolecule microarray
    59.
    发明申请
    Substrate for biomolecule microarray, process for producing the same, biomolecule microarray and method of collecting data on biomolecule microarray 审中-公开
    用于生物分子微阵列的底物，其制备方法，生物分子微阵列和收集生物分子微阵列数据的方法

    公开(公告)号：US20060035220A1

    公开(公告)日：2006-02-16

    申请号：US10494517

    申请日：2002-11-21

    申请人： Hideo Tashiro ,  Yasumitsu Kondoh ,  Tokuji Kitsunai ,  Kaori Honda

    发明人： Hideo Tashiro ,  Yasumitsu Kondoh ,  Tokuji Kitsunai ,  Kaori Honda

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

    摘要： A biomolecular microarray substrate having a spot for fixing a biomolecular probe on a surface thereof. The spot of light-reflecting layer is present between the biomolecular probe-fixing spot and the substrate surface. A method of manufacturing the biomolecular microarray substrate. A biomolecular microarray having a biomolecular probe-fixing spot in which a biomolecular probe is fixed by a biomolecular probe-fixing spot having the biomolecular microarray substrate. A data collection method for biomolecular arrays. Fluorescently labeled target biomolecules is interacted with a biomolecular microarray having, on a substrate surface, biomolecular probe-fixing spots that fix biomolecular probes on light-reflecting layer spots; excitation light is directed onto the biomolecular microarray obtained and reflected light and fluorescence are simultaneously measured from the biomolecular microarray; a biomolecular probe-fixing spot present on a light-reflecting layer spot is specified from differences in the intensity of the light reflecting off of the biomolecular microarray; and fluorescence data are obtained from within the scope of the specified spot. Alternatively, light is directed onto the biomolecular microarray obtained and reflected light is measured from the biomolecular microarray; a biomolecular probe-fixing spot having a light-reflecting layer spot is specified from differences in the intensity of the light reflecting off of the biomolecular microarray; excitation light is directed only onto the scope of the specified biomolecular probe-fixing spot and measuring the fluorescence; and fluorescence data are obtained from the scope of the specified spot.

    摘要翻译： 生物分子微阵列基板，其表面上具有用于固定生物分子探针的斑点。 光反射层的光点存在于生物分子探针固定点和基片表面之间。 制造生物分子微阵列基板的方法。 具有生物分子探针固定点的生物分子微阵列，其中生物分子探针通过具有生物分子微阵列基底的生物分子探针固定点固定。 生物分子阵列的数据采集方法。 荧光标记的目标生物分子与生物分子微阵列相互作用，其在衬底表面上具有将生物分子探针固定在光反射层点上的生物分子探针固定点; 激发光被引导到获得的生物分子微阵列上，并从生物分子微阵列同时测量反射光和荧光; 存在于光反射层点上的生物分子探针固定点由生物分子微阵列反射光的强度的差异来确定; 并且在指定斑点的范围内获得荧光数据。 或者，将光引导到获得的生物分子微阵列上，并从生物分子微阵列测量反射光; 具有光反射层点的生物分子探针固定点由生物分子微阵列的反射光强度的差异来确定; 激发光仅指向指定的生物分子探针固定点的范围并测量荧光; 并且从指定斑点的范围获得荧光数据。